The optimal preparation conditions of Salmon decalcified bone matrix (S-DBM) were explored, and the properties of S-DBM bone particles and bone powder were studied respectively. The therapeutic effect of S-DBM on tibial defect in female Sprague Dawley (SD) rats was preliminarily verified.
Methods:
This study assessed the structural and functional similarities of Salmon bone DBM (S-DBM). The biocompatibility assessment was conducted using both in vivo and in vitro experiments, establishing an animal model featuring tibial defects in rats and on the L929 cell line, respectively. The control group, bovine DBM (bDBM), was compared to the S-DBM-treated tibial defect rats. Imaging and histology were used to study implant material changes, defect healing, osteoinductive repair, and degradation.
Results:
The findings of our study indicate that S-DBM exhibits favorable repairing effects on bone defects, along with desirable physicochemical characteristics, safety, and osteogenic activity.
Conclusions:
The S-DBM holds significant potential as a medical biomaterial for treating bone defects, effectively fulfilling the clinical demands for materials used in bone tissue repair engineering.
BhattacharjeePKunduBNaskarD, et alSilk scaffolds in bone tissue engineering: an overview. Acta Biomater2017; 63: 1–17.
2.
PhukanRHerzogTBolandPJ, et alHow does the level of sacral resection for primary malignant bone tumors affect physical and mental health, pain, mobility, incontinence, and sexual function?Clin Orthop Relat Res2016; 474(3): 687–696.
3.
MajidiniaMSadeghpourAYousefiB.The roles of signaling pathways in bone repair and regeneration. J Cell Physiol2018; 233(4): 2937–2948.
4.
HenkelJWoodruffMAEpariDR, et alBone regeneration based on tissue engineering conceptions—A 21st century perspective. Bone Res2013; 1(1): 216–248.
5.
ZhouGWangFLinG, et alNovel coatings for the continuous repair of human bone defects. Colloids Surf B Biointerfaces2023; 222: 113127.
6.
LiuXTangBLiQ, et alHydrophilic competent and enhanced wet-bond strength castor oil-based bioadhesive for bone repair. Colloids Surf B Biointerfaces2022; 219: 112835.
7.
BarcakEABeebeMJWeinleinJC.The role of implant removal in orthopedic trauma. Orthop Clin North Am2018; 49(1): 45–53.
8.
TianLTangNNgaiT, et alHybrid fracture fixation systems developed for orthopaedic applications: a general review. J Orthop Translat2019; 16: 1–13.
9.
HanXZhouXQiuK, et alStrontium-incorporated mineralized PLLA nanofibrous membranes for promoting bone defect repair. Colloids Surf B Biointerfaces2019; 179: 363–373.
10.
De PonteFSFalzeaRRunciM, et alHistomorhological and clinical evaluation of maxillary alveolar ridge reconstruction after craniofacial trauma by applying combination of allogeneic and autogenous bone graft. Chin J Traumatol2017; 20(1): 14–17.
11.
PanY-XYangG-GLiZ-W, et alClinical observation of biomimetic mineralized collagen artificial bone putty for bone reconstruction of calcaneus fracture. Regen Biomater2018; 5(2): 61–67.
12.
DuJZhouYBaoX, et alSurface polydopamine modification of bone defect repair materials: Characteristics and applications. Front Bioeng Biotechnol2022; 10: 974533.
13.
ChoHBucciarelliAKimW, et alNatural sources and applications of demineralized bone matrix in the field of bone and cartilage tissue engineering. Adv Exp Med Biol2020; 1249: 3–14.
14.
HaoJBaiBCiZ, et alLarge-sized bone defect repair by combining a decalcified bone matrix framework and bone regeneration units based on photo-crosslinkable osteogenic microgels. Bioact Mater2022; 14: 97–109.
15.
HuQLiuMChenG, et alDemineralized bone scaffolds with tunable matrix stiffness for efficient bone integration. ACS Appl Mater Interfaces2018; 10(33): 27669–2780.
16.
ChenBLinHWangJ, et alHomogeneous osteogenesis and bone regeneration by demineralized bone matrix loading with collagen-targeting bone morphogenetic protein-2. Biomaterials2007; 28(6): 1027–1035.
17.
SaloniusEKontturiLLaitinenA, et alChondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment. J Cell Physiol2020; 235(4): 3497–3507.
18.
Caballé-SerranoJZhangSSculeanA, et alTissue integration and degradation of a porous collagen-based scaffold used for soft tissue augmentation. Materials2020; 13(10): 2420.
19.
WardBBBrownSEKrebsbachPH.Bioengineering strategies for regeneration of craniofacial bone: a review of emerging technologies. Oral Dis2010; 16(8): 709–716.
20.
Madhurakkat PerikamanaSKLeeJLeeYB, et alMaterials from mussel-inspired chemistry for cell and tissue engineering applications. Biomacromolecules2015; 16(9): 2541–2555.
21.
TaskinMBXuRGregersenH, et alThree-dimensional polydopamine functionalized coiled microfibrous scaffolds enhance human mesenchymal stem cells colonization and mild myofibroblastic differentiation. ACS Appl Mater Interfaces2016; 8(25): 15864–15873.
22.
CesurSOktarFNEkrenN, et alPreparation and characterization of electrospun polylactic acid/sodium alginate/orange oyster shell composite nanofiber for biomedical application. J Aust Ceram Soc2020; 56: 533–543.
23.
BoseSRoyMBandyopadhyayA.Recent advances in bone tissue engineering scaffolds. Trends Biotechnol2012; 30(10): 546–554.
24.
ZhangX-YChenY-PHanJ, et alBiocompatible silk fibroin/carboxymethyl chitosan/strontium substituted hydroxyapatite/cellulose nanocrystal composite scaffolds for bone tissue engineering. Int J Biol Macromol2019; 136: 1247–1257.
25.
ZhangDHouJGuY, et alCryopreserved skin epithelial cell sheet combined with acellular amniotic membrane as an off-the-shelf scaffold for urethral regeneration. Mater Sci Eng C2021; 122: 111926.
26.
ShuaiCYuanXYangW, et alSynthesis of a mace-like cellulose nanocrystal@Ag nanosystem via in-situ growth for antibacterial activities of poly-L-lactide scaffold. Carbohydr Polym2021; 262: 117937.
27.
ShuaiCYangWFengP, et alAccelerated degradation of HAP/PLLA bone scaffold by PGA blending facilitates bioactivity and osteoconductivity. Bioact Mater2021; 6(2): 490–502.
28.
YangWTongQHeC, et alMechanically propelled ion exchange regulates metal/bioceramic interface characteristics to improve the corrosion resistance of Mg composite for orthopedic applications. Ceram Int2024; 50(13, Part A): 23124–23134.
29.
GengZLiZCuiZ, et alNovel bionic topography with MiR-21 coating for improving bone-implant integration through regulating cell adhesion and angiogenesis. Nano Lett2020; 20(10): 7716–7721.
30.
GordonTBrushartTMChanKM.Augmenting nerve regeneration with electrical stimulation. Neurol Res2008; 30(10): 1012–1022.
31.
SongJSunBLiuS, et alPolymerizing pyrrole coated poly (l-lactic acid-co-ε-caprolactone)(PLCL) conductive nanofibrous conduit combined with electric stimulation for long-range peripheral nerve regeneration. Front Mol Neurosci2016; 9: 117.
32.
YannasIV.Emerging rules for inducing organ regeneration. Biomaterials2013; 34(2): 321–330.
33.
TainioJPaakinahoKAholaN, et alIn vitro degradation of borosilicate bioactive glass and poly(l-lactide-co-ε-caprolactone) composite scaffolds. Materials2017; 10(11): 1274.
