Polyaromatic Hydrocarbons Administered in Humans by Dermal Route Increase Total IgE

Abstract

Inhalation of polyaromatic hydrocarbons (PAHs) extracted from diesel exhaust particles (DEP) enhances local (nasal) production of IgE in humans. The aim of the present research is to investigate whether in humans dermal exposure to PAHs which are not extracted from DEPs increases serum IgE and whether host factors modify the immunologic effect. In thirty-two patients with acute psoriatic lesions, a cream containing 3% of coal tar (which holds a variety of PAHs) was applied to the skin for 24 hours. Serum IgE were measured before (IgE₀) and four (IgE₄) and eight (IgE₈) days after application. Replicated means were compared by analysis of variance for repeated measures and by the Newman-Keuls' test. IgE₀, IgE₄ and IgE₈ were 151.19, 159.69 (a 6% excess) and 170.90 kU/L (a 13% excess) respectively; pairwise comparison showed IgE₈ was significantly higher than IgE₀ (p<0.05). At multiple linear regression analysis, the percentage increase in serum IgE across observation days was the dependent variable against age, sex, cigarettes/day, urinary 1-pyrenol, atopy, skin area treated, and grams of cream. Of the independent variables, only age had a significant (p<0.028) influence: the younger the age, the higher the IgE response to PAHs. We conclude that whatever the source and the route of entry (skin or respiratory tract), PAHs increase total serum IgE, mainly in younger age groups.

Keywords

polyaromatic hydrocarbons total IgE allergic diseases psoriasis coal tar cream humans

References

Takafuji

Suzuki

Koizumi

Tadokoro

Ohashi

Muranaka

. 1989. Enhancing effect of suspended particulate matter on the IgE antibody production in mice. Int. Arch. Allergy Appl. Immunol. 90:1.

Diaz-Sanchez

Dotson

A.R.

Takenaka

and Saxon

. 1994. Diesel exhaust particles induce local IgE production in vivo and alter the pattern of IgE messenger RNA isoforms. J. Clin. Invest. 94:1417.

Takenaka

Zhang

Diaz-Sanchez

Tsien

and Saxon

. 1995. Enhanced human IgE production results from exposure to the aromatic hydrocarbons from diesel exhaust: Direct effects on B-cell IgE production. J. Allergy Clin. Immunol. 95:103.

Talaska

Underwood

Maier

Lewtas

Rothman

and Jaeger

. 1996. Polycyclic aromatic hydrocarbons (PAHs), nitro-PAHs and related environmental compounds: Biological markers of exposure and effects. Environ. Health. Perspect. 104; (S) 5:901.

Kang

D.H.

Correa-Villasensor

Breyssea

P.N.

and Strickland

P.T.

. 1995. Correlation of urinary 1-hydroxypyrene glucuronide concentration between mothers and their children. Proc. Annu. Meet. Am. Assoc. Cancer Res. 36(S):107.

Weigl

B.A.

1998. Immunoregulatory mechanisms and stress hormones in psoriasis (part 1). Int. J. Dermatol. 37:350.

Jongeneelen

F. J.

Azion

R.B.M.

and Henderson

P.T.

. 1987. Determination of hydroxylated metabolites of polycyclic aromatic hydrocarbons in urine. J. Chrom. 431:227.

Dekker

F.W.

Mulder-Dzn

J.D.

Kramps

J.A.

Kaptein

A.A.

Vandenbroucke

J.P.

and Dijkman

J.H.

. 1990. The Phadiatop in vitro test for allergy in general practice: is it useful ? Fam Pract. 7:144.

Matricardi

P.M.

Nisini

Pizzolo

J.G.

and D'Amelio

. 1990. The use of Phadiatop in mass-screening programmes of inhalant allergies: advantages and limitations. Clin. Exp. Allergy. 20:151.

10.

Winer

B.J.

1971. Statistical principles in experimental design, 2nd ed.

McGraw-Hill Book Inc.

New York, p. 1.

11.

Pavanello

Genova

Foa

and Clonfero

. 2000. Assessment of occupational exposure to aromatic polycyclic hydrocarbons determining urinary levels of 1-pyrenol. Med. Lav. 91:192.

12.

Jongeneelen

F.J.

1992. Biological exposure limit for occupational exposure to coal tar pitch volatiles at cokeovens. Int. Arch. Occup. Environ. Health. 63:511.

13.

Jensen

E.J.

Pedersen

Schmidt

and Dahl

. 1992. Serum IgE in nonatopic smokers, nonsmokers, and recent exsmokers: relation to lung function, airway symptoms, and atopic predisposition. J. Allergy Clin. Immunol. 90:224.

14.

Basketter

D.A.

Miettinen

and Lahti

. 1998. Acute irritant reactivity to sodium lauryl sulfate in atopics and non-atopies. Contact Dermatitis. 38:253.

15.

Manetz

T.S.

Pettit

D.A.

and Meade

B.J.

. 2001. The determination of draining lymph node cell cytokine mRNA levels in BALB/c mice following dermal sodium lauryl sulfate, dinitrofluorobenzene, and toluene diisocyanate exposure. Toxicol. Appl. Pharmacol. 171:174.

16.

Jones

C.D.

Guckian

el Ghorr

A.A.

Gibbs

N.K.

and Norval

. 1996. Effects of phototherapy on the production of cytokines by peripheral blood mononuclear cells and on systemic antibody responses in patients with psoriasis. Photodermatol. Photoimmunol. Photomed. 12:204.

17.

Peterson

and Saxon

. 1996. Global increases in allergic respiratory disease: the possible role of diesel exhaust particles. Ann. Allergy Asthma Immunol. 77:263.

18.

Diaz-Sanchez

1997. The role of diesel exhaust particles and their associated polyaromatic hydrocarbons in the induction of allergic airway disease. Allergy 52(S38):52.

19.

Tsien

Diaz-Sanchez

and Saxon

. 1997. The organic component of diesel exhaust particles and phenanthrene, a major polyaromatic hydrocarbon constituent, enhances IgE production by IgE-secreting EBV-transformed human 13 cells in vitro. Toxicol. Appl. Pharmacol. 142:256.

20.

Diaz-Sanchez

Tsien

Casillas

Dotson

A.R.

and Saxon

. 1996. Enhanced nasal cytokine production in human beings after in vivo challenge with diesel exhaust particles. J. Allergy. Clin. Immunol. 98:114.

21.

Diaz-Sanchez

Tsien

Fleming

and Saxon

. 1997. Combined diesel exhaust particulate and ragweed allergen challenge markedly enhances human in vivo nasal ragweed-specific IgE and skews cytokine production to a T helper cell 2-type pattern. J. Immunol. 158:2406.

22.

Barfknecht

T.R.

Hites

R.A.

Cavaliers

E.L.

and Thilly

W.G.

. 1982. Human cell mutagenicity of polycyclic aromatic hydrocarbon components of diesel emissions. Dev. Toxicol. Environ. Sci. 102:77.

23.

Clonfero

Zordan

Cottica

Venier

Pozzoli

Cardin

E.L.

. 1986. Mutagenic activity and polycyclic aromatic hydrocarbon levels in urine of humans exposed to therapeutical coal tar. Carcinogenesis 7:819.

24.

Bauer

S.L.

and Howard

P.C.

. 1991. Kinetics and cofactor requirements for the nitroreductive metabolism of 1-nitropyrene and 3-nitrofluoranthene by rabbit liver aldehyde oxidase. Carcinogenesis 12:1545.