This study aimed to investigate the effects of a novel patented Mg-3Nd-0.2Zn-0.4Zr (weight %, JDBM) alloy on osteoblastic cell function, as these cells play an important role in bone repair and remodeling. The associated effects of the JDBM alloy on osteoblastic cell function involving cell adhesion, cell proliferation, and mineralization were investigated using scanning electron microscopy (SEM), MTT assay and ambramycin staining, respectively. At the same time, the in vitro degradation behavior of the JDBM alloy in cell culture medium was evaluated by the weight-loss method and SEM. Pure magnesium was used as control. The results showed that osteoblastic cells cultured on JDBM alloy samples manifested better cell adhesion, improved cell proliferation and increased mineralization ability, compared with cells seeded on pure magnesium samples. Our data indicate that the JDBM alloy has excellent bioactivity, improving the cell function of osteoblastic cells seeded on it.
WitteF. The history of biodegradable magnesium implants: A review. Acta Biomaterialia2010; 6:1680–92.
2.
StaigerMPPietakAMHuadmaiJDiasG. Magnesium and its alloys as orthopedic biomaterials: A review. Biomaterials2006; 27:1728–34.
3.
WitteFFischerJNellesenJCrostackHAKaeseVPischABeckmannFWindhagenH. In vitro and in vivo corrosion measurements of magnesium alloys. Biomaterials2006; 27:1013–18.
4.
WangYZhuZHeYJiangYZhangJNiuJMaoLYuanG. In vivo degradation behavior and biocompatibility of Mg-Nd-Zn-Zr alloy at early stage. Int J Mol Med2012; 29:178–84.
5.
XuLPanFYuGYangLZhangEYangK. In vitro and in vivo evaluation of the surface bioactivity of a calcium phosphate coated magnesium alloy. Biomaterials2009; 30:1512–23.
6.
ZbergBUggowitzerPJLöfflerJF. MgZnCa glasses without clinically observable hydrogen evolution for biodegradable implants. Nature Materials2009; 8:887–91.
7.
LiZGuXLouSZhengY. The development of binary Mg-Ca alloys for use as biodegradable materials within bone. Biomaterials2008; 29: 1329–44.
8.
WeiJJiaJWuFWeiSZhouHZhangHShinJLiuC. Hierarchically microporous/macroporous scaffold of magnesium-calcium phosphate for bone tissue regeneration. Biomaterials2010; 31:1260–9.
9.
WangYHeYZhuZJiangYZhangJNiuJMaoLYuanG. In vitro degradation and biocompatibility of Mg-Nd-Zn-Zr alloy. Chin Sci Bull2012; 57:2163–70.
10.
XuLZhangEYinDZengSYangK. In vitro corrosion behaviour of Mg alloys in a phosphate buffered solution for bone implant application. J Mater Sci Mater Med2008; 19:1017–25.
11.
ZhangSLiJSongY. In vitro degradation, hemolysis and MC3T3-E1 cell adhesion of biodegradable Mg-Zn alloy. Materials Science and Engineering C2009; 29:1907–12.
12.
ZhangSZhangXZhaoCLiJSongYXieCTaoHZhangYHeYJiangYBianY, Research on an Mg-Zn alloy as a degradable biomaterial. Acta Biomaterialia2010; 6:626–40.
13.
WongHMYeungKWLamKOTamVChuPKLukKDCheungKM. A biodegradable polymer-based coating to control the performance of magnesium alloy orthopaedic implants. Biomaterials2010; 31:2084–96.
14.
YangJCuiFLeeIS. Surface modifications of magnesium alloys for biomedical applications. Ann Biomed Eng2011; 39:1857–71.
15.
YinDZhangEZengS. Effect of Zn on mechanical property and corrosion property of extruded Mg-Zn-Mn alloy. Trans Nonferrous Metals Soc2008; 18:763–8.
16.
ZhangEYangLXuJChenH. Microstructure, mechanical properties and bio-corrosion properties of Mg-Si (-Ca, Zn) alloy for biomedical application. Acta Biomater2010; 6:1756–62.
17.
HeYTaoHZhangY. Biocompatibility of bio-Mg-Zn alloy within bone with heart, liver, kidney and spleen. Chin Sci Bull2009; 54:484–91.
18.
ZhangXYuanGNiuJFuPDingW. Microstructure, mechanical properties, biocorrosion behavior, and cytotoxicity of as-extruded Mg-Nd-Zn-Zr alloy with different extrusion ratios. J Mech Behav Biomed Mater2012; 9:153–62.
19.
WitteFFischerJNellesenJVogtCVogtJDonathTBeckmannF. In vivo corrosion and corrosion protection of magnesium alloy LAE442. Acta Biomater2010; 6:1792–9.
20.
BoehlertCJKnittelK. The microstructure, tensile properties, and creep behaviour of Mg–Zn alloys containing 0–4.4 wt% Zn. Mater Sci Eng A2006; 417:315–21.
21.
BabkinVM. Effect of zirconium on the grain size of magnesium containing 4.5% Zn. Met Sci Heat Treat2004; 5:543–4.
22.
SongGJohnD. The effect of zirconium grain refinement on the corrosion behavior of magnesium-rare earth alloy MEZ. J Light Met2002; 1:1–16.
23.
RudeRKGruberHEWeiLYFraustoAMillsBG. Magnesium deficiency: Effect on bone and mineral metabolism in the mouse. Calcif Tissue Int2003; 72:32–41.
24.
HarryR. Magnesium: The missing element in molecular views of cell proliferation control. Bioassays2005; 27:311–20.
25.
YamaguchiMOishiHSuketaY. Stimulatory effect of zinc on bone formation in tissue culture. Biochem Pharmacol1987; 36:4007–12.
26.
ChengKWengWWangHZhangS. In vitro behavior of osteoblast-like cells on fluoridated hydroxyapatite coatings. Biomaterials2005; 26:6288–95.
27.
VillaIDal FiumeCMaestroniARubinacciARavasiFGuidobonoF. Human osteoblast-like cell proliferation induced by calcitonin-related peptides involves PKC activity. Am J Physiol Endocrinol Metab2003; 284:E627–33.
28.
HynesRO. Integrins: Versatility, modulation, and signaling in cell adhesion. Cell1992; 69:11–25.
29.
PaulWSharmaCP. Nanoceramic matrices: Biomedical applications. Am J Biochem Biotechnol2006; 2:41–48.
30.
ZreiqatHHowlettCRZannettinoAEvansPSchulze-TanzilGKnabeCShakibaeiM. Mechanisms of magnesium-stimulated adhesion of osteoblastic cells to commonly used orthopaedic implants. J Biomed Mater Res2002; 62:175–84.
31.
WitteFHortNVogtCCohenSKainerKUWillumeitRFeyerabendF. Degradable biomaterials based on magnesium corrosion. Curr Op Solid State Mat Science2008; 12:63–72.
32.
SongGL. Control of biodegradation of biocompatible magnesium alloys. Corr Sci2007; 49:1696–701.
