Diagnosis of CGD is made by demonstrating absent or markedly reduced oxidase activity in stimulated neutrophils. The screening test proposed is based upon the naked eye evaluation of the reduction of NBT on a solid surface. It seems to be a useful tool for rapid and inexpensive detection of CGD patients, especially for large-scale screening purposes. The test was carried out on forty-five subjects: two males affected by CGD, three female carriers and forty healthy donors. The test confirmed the results obtained with flow cytometric and NBT assays.
ParkBHFikrigSMSmithwickEM. Infection and nitroblue-tetrazolium reduction by neutrophils. Lancet1968; 2:532–34.
2.
OchsHDIgoRP. The NBT slide test: a simple screening method for detecting chronic granulomatous disease and female carriers. J Pediatr1973; 83:77–82.
3.
IacobiniMTorreAPalumboGDel PrincipeDDigilioGBalducciL. Study of chronic granulomatous disease by a nitroblue tetrazolium densitometric kinetic test: a new research method. Clinica Chimica Acta2211993; 197–202.
4.
JirapongsananurukOMalechHLKuhnsDBNiemelaJEBrownMRAnderson-CohenM, and FleisherTA. Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay. J Allergy Clin Immunol2003; 111:374–9.
5.
BalducciLBattagliaMMidullaM. Prova diagnostica rapida per la malattia granulomatosa cronica (Rapid diagnostic test for chronic granulomatous disease). Boll Soc Ital Biol Sper1975; 51:499–504.
