Metal working fluids (MWFs) are widely used in industry for metal cutting, drilling, shaping, lubricating, and milling. Potential for dermal exposure to MWFs exists for a large number of men and women via aerosols and splashing during the machining operations. It has been reported earlier that occupational exposure to MWFs causes allergic and irritant contact dermatitis. Previously, we showed that dermal exposure of female and male B6C3F1 mice to 5% MWFs for 3 months resulted in accumulation of mast cells and elevation of histamine in the skin. Topical exposure to MWF also resulted in elevated oxidative stress in the liver of both sexes and the testes in males. The goal of this study was to evaluate the interaction between oxidative stress in the skin and topical application of MWF. Oxidative stress in skin of B6C3F1 mice of both sexes was generated by intradermal injection of the hydrogen peroxide (H2O2) -producing enzyme, glucose oxidase with polyethylene glycol (GOD+PEG). In mice given GOD+PEG, topical treatment with MWF (200 l, 30%, for 1, 3, or 7 days) resulted in a mixed inflammatory cell response, accumulation of peroxidative products, and reduction of GSH content in the skin. Such changes were not observed with MWF treatment alone. These data indicate that oxidative stress can enhance dermal inflammation caused by occupational exposure to MWF.
Al-Humadi N. H., Battelli L., Willard P. A., Schwegler-Berry D., Castranova V., and Kommineni C.Effects of metal working fluids on B6C3F1 mouse skin. Toxicol Ind Health2000a: 16: 1-8.
2.
Al-Humadi N. H., Shvedova A. A., Battelli L., Diotte N., Castranova V., and Kommineni C.Dermal and systemic toxicity after application of semisynthetic metal-working fluids in B6C3F1 mice. J Toxicol Environ Health2000b: 61 (7): 579-589.
Alomar A., Conde-Salazar L., and Romaguera C.Occupational dermatoses from cutting oils. Contact Dermatitis1985: 12: 129-138.
5.
Applegate L. A., Frenk E., Gibbs N., Johnson B., Ferguson J., and Tyrrell R. M.Cellular sensitivity to oxidative stress in the photosensitivity dermatitis/actinic reticuloid syndrome. J Invest Dermatol1994: 102 (5): 762-767.
6.
Benn S. I., Whitsitt J. S., Broadley K. N., Nanney L. B., Perkins D., He L., Patel M., Morgan J., Swain W. F., and Davidson J. M.Particle-mediated gene transfer with transforming growth factor-beta1 cDNAs enhances wound repair in rat skin. J Clin Invest1996: 98 (12): 2894-2902.
7.
Boh E. E.Role of reactive oxygen species in dermatologic diseases. Clin Dermatol1996: 14 (4): 343-352.
8.
Buege J. A., and Aust S. D.Microsomal lipid peroxidation. Methods Enzymol1978: 52: 302-310.
9.
Camarasa J. G., Romaguera C., Serra-Baldrich E., and Vilaplana J.Allergic contact dermatitis from Biobans in Spanish metal workers. Contact Dermatitis1993: 29 (2): 98-98.
10.
Cetinkale O., Senel O., and Bulan R.The effect of antioxidant therapy on cell mediated immunity following burn injury in an animal model. Burns1999: 25 (2): 113-118.
11.
Dahlquist I.Contact allergy to the cutting oil preservatives Bioban CS-1246 and P-1487. Contact Dermatitis1984: 10 (1): 46-46.
12.
Damstra R. J., Van Vloten W. A., and Van Ginkel C. J. W.Allergic contact dermatitis from the preservative 1,2-benzisothiazolin-3-one (1,2- BT; Proxel1): a case report, its prevalence in those occupationally at risk and in the general dermatological population, and its relationship to allergy to its analogue Kathon1 CG. Contact Dermatitis1992: 27: 105-109.
13.
De Boer E. M., Bruynzeel D. P., and van Ketel W. G.Dyshidrotic eczema as an occupational dermatitis in metal workers. Contact Dermatitis1988: 19 (3): 184-188.
14.
De Boer E. M., van Ketel W. G., and Bruynzeel D. P.Dermatoses in metal workers. Irritant contact dermatitis. Contact Dermatitis1989a: 20: 212-218.
15.
De Boer E. M., van Ketel W. G., and Bruynzeel D. P.Dermatoses in metal workers. Allergic contact dermatitis. Contact Dermatitis1989b: 20: 280-286.
16.
DOL. Occupational injuries and illnesses in the United States by industry, 1986. Washington, DC: U.S. Department of Labor, Bureau of Labor Statistics. U.S. Government Printing Office, Bulletin 2308, 1988.
17.
DOL. Occupational injuries and illnesses in the United States by industry, 1987. Washington, DC: U.S. Department of Labor, Bureau of Labor Statistics. U.S. Government Printing Office, Bulletin 2328, 1989.
18.
DOL. Occupational injuries and illnesses in the United States by industry, 1991. Washington, DC: U.S. Department of Labor, Bureau of Labor Statistics. U.S. Government Printing Office, Bulletin 2424, 1993.
19.
Freitas J. P., Filipe P., Yousefi A., Emerit I., and Guerra Rodrigo F.Oxidative stress in Adamantiades Behcet’s disease. Dermatology1998: 197 (4): 343-348.
20.
Fuchs J.Potentials and limitations of the natural antioxidants RRR-alphatocopherol, L -ascorbic acid and beta-carotene in cutaneous photo-protection. Free Radic Biol Med1998: 25 (7): 848-873.
21.
Gallucci R. M., Simeonova P. P., Matheson J. M., Kommineni C., Guriel J. L., Sugawara T., and Luster M. I.Impaired cutaneous wound healing in interleukin-6-deficient and immunosuppressed mice. FASEB J2000: 14 (15): 2525-2531.
22.
Gow A. J., Branco F., Christofidou-Solomidou M., Black-Schultz L.,Albelda S. M., and Muzykantov V. R.Immunotargeting of glucose oxidase: intracellular production of H2O2 and endothelial oxidative stress. Am J Physiol1999: 277 (2 Pt 1): L271-L281.
23.
Hirai A., Minamiyama Y., Hamada T., Ishii M., and Inoue M.Glutathione metabolism in mice is enhanced more with hapten-induced allergic contact dermatitis than with irritant contact dermatitis. J Invest Dermatol1997: 109 (3): 314-318.
24.
McCord J. M.Human disease, free radicals, and the oxidant/antioxidant balance. Clin Biochem1993: 26 (5): 351-357.
25.
McCord J. M., Wong K., Stokes S. H., Perone W. F., and English D.Superoxide and inflammation: a mechanism for the anti-inflammatory activity of superoxide dismutase. Acta Physiol Scand Suppl1980: 492: 25-30.
26.
Mendelow A. Y., Forsyth A., Feather J. W., Baillie A. J., and Florence A. T.Skin reflectance measurements of patch test responses. Contact Dermatitis1986: 15 (2): 73-78.
27.
Moore K.Cell biology of chronic wounds: the role of inflammation. J Wound Care1999: 8 (7): 345-348.
Muzykantov V. R., Sakharov D. V., Sinitsyn V. V., Domogatsky S. P., Goncharov N. V., and Danilov S. M.Specific killing of human endothelial cells by antibody-conjugated glucose oxidase. Anal Biochem1988: 169 (2): 383-389.
30.
Nachbar F., and Korting H. C.The role of vitamin E in normal and damaged skin. J Mol Med1995: 73 (1): 7-17.
31.
Nakamura Y., Murakami A., Ohto Y., Torikai K., Tanaka T., and Ohigashi H.Suppression of tumor promoter-induced oxidative stress and inflammatory responses in mouse skin by a superoxide generation inhibitor 10 -acetoxychavicol acetate. Cancer Res1998: 58 (21): 4832-3839.
32.
NIOSH. Criteria for a recommended standard: occupational exposure to metal working fluids. Cincinnati, OH: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, DHHS (NIOSH)1998.
33.
Panayiotidis M., Tsolas O., and Dalaris D.Glucose oxidase-produced H2O2 induces Ca2+ -dependent DNA damage in human peripheral blood lymphocytes. Free Radic Biol Med1999: 26 (5-6): 548-556.
34.
Passi S., Grandinetti M., Maggio F., Stancato A., and De Luca C.Epidermal oxidative stress in vitiligo. Pigm Cell Res1998: 11 (2): 81-85.
35.
Petrone W. F., English D. K., Wong K., and McCord J. M.Free radicals and inflammation: superoxide-dependent activation of a neutrophil chemotactic factor in plasma. Proc Natl Acad Sci USA1980: 77(2): 1159-1163.
36.
Picardo M., Zompetta C., Marchese C., De Luca C., Faggioni A., Schmidt R. J., and Santucci B.Paraphenylenediamine, a contact allergen, induces oxidative stress and ICAM-1 expression in human keratinocytes. Br J Dermatol1992: 126 (5): 450-455.
37.
Rezazadeh H., and Athar M.Evidence that iron overload promotes 7,12- dimethylbenz(a)anthracene-induced skin tumorigenesis in mice. Redox Rep1997: 3 (5-6): 303-309.
38.
Rycroft R. J. G.Petroleum and petroleum derivatives. In: Adams R. M., ed. Occupational Skin Disease. 2nd ed. W.B. Saunders: Philadelphia, PA; 1990: 486-502.
39.
Salazar J. J., and Houten B.Preferential mitochondrial DNA injury caused by glucose oxidase as a steady generator of hydrogen peroxide in human fibroblasts. Mutat Res1997: 385 (2): 139-149.
40.
Schallreuter K. U., Moore J., Wood J. M., Beazley W. D., Gaze D. C., Tobin D. J., Marshall H. S., Panske A., Panzig E., and Hibberts N. A.In vivo and in vitro evidence for hydrogen peroxide (H2O2) accumulation in the epidermis of patients with vitiligo and its successful removal by an UVB-activated pseudocatalase. J Invest Dermatol1999: 4 (1): 91-96.
41.
Shingu M., Nonaka S., Nobunaga M., and Ahamadzadeh N.Possible role of H2O2 -mediated complement activation and cytokines-mediated fibroblasts superoxide generation on skin inflammation. Dermatologica1989: 179 (suppl 1): 107-112.
42.
Shvedova A. A., Kommineni C., Jeffries B. A., Castranova V., Tyurina Y. Y., Tyurin V. A., Serbinova E. A., Fabisiak J. P., and Kagan V. E.Redox cycling of phenol induces oxidative stress in human epidermal keratinocytes. J Invest Dermatol2000: 114: 354-364.
43.
Shvedova A. A., Tyurina Y. Y., Gorbunov N. V., Tyurin V. A., Castranova V., Kommineni C., Ojimba J., Gandley R., McLaughlin M. K., and Kagan V. E. tert -Butyl hydroperoxyde/hemoglobin-induced oxidative stress and damage to vascular smooth muscle cells. Biochem Pharmacol1999: 57: 989-1001.
44.
Singer A. J., and Clark R. A. F.Cutaneous wound healing. N Engl J Med1999: 341: 10, 738-746.
45.
Sprince N., Thorne P. S., and Cullen M. R.Oils and related petroleum derivatives. In: Rosenstock L., Cullen M. R., eds. Textbook of Clinical Occupational and Environmental Medicine. W.B. Saunders: Philadelphia, PA; 1994: 814-824.
46.
Sprince N. L., Palmer J. A., Popendorf W., Thorne P. S., Selim M. I., Zwerling C., and Miller ER.Dermatitis among automobile production machine operators exposed to metal working fluids. Am J Ind Med1996: 30: 421-429.
47.
Trenam C. W., Dabbagh A. J., Blake D. R., and Morris C. J.The role of iron in an acute model of skin inflammation induced by reactive oxygen species (ROS). Br J Dermatol1992: 126: 250-256.
48.
Trenam C. W., Dabbagh A. J., Morris C. J., and Blake D. R.Skin inflammation induced by reactive oxygen species (ROS): an in-vivo model. Br J Dermatol1991: 125: 325-329.
49.
Wardman P., and Candeias L. P.Fenton chemistry: an introduction. Radiat Res1996: 145 (5): 523-531.
50.
Zugerman C.Cutting fluids. Their use and effects on the skin. Occup Med: State of the Art Rev1986: 1 (2): 245-258.
