Affects of ionizing radiation on T-cell population lymphocyte: A risk factor of Irritable Bowel Syndrome

Abstract

Irritable bowel syndrome is observed mostly in Ukrainian children and may be related to adverse health effects as a result of the Chernobyl disaster. The aim of this study was to determine status of T-cell population lymphocytes in children with clinical symptom of irritable bowel syndrome. The test population consisted of 95 participants: 75 rural patients aged 4 to 18 who lived in a contaminated area exposed to natural environmental radiation with clinical symptom of irritable bowel syndrome (categorized in three groups) and 20 healthy urban participants from Kiev aged 5 to 15 as control group. Internal radiation activity has been measured by γ-ray spectrometry. Peripheral blood leukocytes were analyzed for T-lymphocytes subset such as T-lymphocytes (CD3⁺), T-helper (CD4⁺) and T-cytotoxic (CD8⁺) and then CD4/CD8 ratio was calculated. Percentage of CD3⁺ and CD4⁺ in all study groups decreased significantly in comparison to control group (p < 0.001). Percentage of CD8⁺ increased significantly in all study groups in comparison to control group (p < 0.05). The CD4/CD8 ratio decreased significantly all study groups in comparison to control group (p < 0.001). Cellular immune status of study groups that are residing in a contaminated area has changed. Our data have demonstrated that this change may be related to a higher incidence of children with irritable bowel syndrome.

Keywords

ionizing radiation immune status irritable bowel syndrome Chernobyl children

Get full access to this article

View all access options for this article.

References

Akira O. , Ryuji O. , and Toshiyuki N. ( 2003) Effect of extended exposure to low-dose radiation on autoimmune diseases of immunologically suppressed MRL/ MpTn-gld/gld mice. Radiation Research 44: 243-247.

Chadwick VS , Chen W. , Shu D. , et al. (2002) Activation of the mucosal immune system in irritable bowel syndrome. Gastroenterology 122: 1778-1783.

Cheryl A. , Carole A. ( 2006) Hostile planet: the essential guide to surviving disaster, pandemic and terrorist attacks. 1st (edn.) Rooftop Publishing , 357.

Chumak A. , Tevenon C. and Gulaya N. ( 2001) Monohydroxylated fatty acid content in peripheral blood mononuclear cells and immune status of people at long times after the Chernobyl accident. Radiat Res 156: 476-487.

Collins SM ( 1996) The immunomodulation of enteric neuromuscular function: implications for motility and inflammatory disorders. Gastroenterology 111: 1683-1699.

Environmental consequences of the Chernobyl accident and their remediation: twenty years of experience. Report of the Chernobyl Forum Expert Group ‘Environment. (STI/PUB/1239), Austria , IAEA, 2006.

Galizeko A. ( 1990) Evaluation of immune system of children in zone of heightening radiation (in Russian). Zdravookhv Beloruss. 6: 33-35.

Golikov V. , Balonov M. , and Jacob P. ( 2002) External exposure of the population living in areas of Russia contaminated due to the Chernobyl accident. Radiation and Environment Biophysics 41: 185-193.

Isakov N. , Scholtz W. , and Altman A. ( 1986) Signal transduction and intracellular events in t-lymphocyte activation. Immunology Today 7: 271-277.

10.

Ishinara H. , suneoka KT , and Dimchev A. ( 1993) Induction of the expression of the interleukine-1 beta gene in mouse spleen by ionizing radiation. Radiation Research 133: 321-326.

11.

James S. , Makinodan T. ( 1988) T cell potentiation in normal and autoimmune-prone mice after extended exposure to low doses of ionizing radiation and/or caloric restriction. International Journal of Radiation Biology 53: 137-152.

12.

Karanta A. , Konithy E. , and Jondal M. ( 1992) Mitogen stimulation increases c-fos and c-jun protein levels, AP-1 binding and AP-1 transcription activity. Cell Signal 4: 275-286.

13.

Kurjane N. , Bruvere R. , Shitova O. , and Romanova T. ( 2002) Analysis of the immune status in latvian Chernobyl clean-up workers with nononcological thyroid diseases. Scandinavian Journal of Immunology 54: 528-533.

14.

Kuzmenok O. , Potapnev M. , and Potapova S. ( 2003) Late effects of the Chernobyl radiation accident on T cell-mediated immunity in cleanup workers. Radiation Research 159: 109-116.

15.

Ohman L. , Isaksson S. , Lindmark AC , Posserud I. , Stotzer PO , Strid H. , Sjövall H. , and Simrén M. (2009) T-cell activation in patients with irritable bowel syndrome. American Journal of Gastroenterology 104: 1205-1212.

16.

Reshetnikov OV , Kurilovich SA , Denisova DV , Zav’ialova LG , Svetlova IO , Tereshonok IN , et al. (2001) Prevalence and risk factors of the development of irritable bowel syndrome in adolescents: a population study. Terapevticheskii Arkhiv 73: 24-29.

17.

Sanchez AL , Walters CB , Singleton DL , Wood NH , and Mondon K. ( 1999) Food ingestion doses from artificial radionuclides in Cumbrian diets, ten years post Chernobyl. Journal of Environmental Radioactivity 46: 301-317.

18.

Sources and Effects of Ionizing Radiation (Report to the General Assembly) (2000) Scientific Committee on the Effects of Atomic Radiation. New York: UN.

19.

Spiller RC ( 2003) Postinfectious irritable bowel syndrome. Gastroenterology 124: 1662-1671.

20.

Titova LD , Oradovskaia IV , and Sharova NI ( 1996) Comparative evaluation of the content of T-lymphocyte subpopulations, alpha 1-thymosin and autoantibodies to epithelial thymic cells in the personnel in the 30-kilometer control zone of the accident at the Chernobyl Atomic Electric Power Station. Radiatsionnaia Biologiia, Radioecologiia 36: 601-609.

21.

Van Der Veek PP , Van Den Ber M. , De Kroon YE , et al. (2005) Tumor necrosis factor-alpha and interleukin 10 gene polymorphisms in irritable bowel syndrome. American Journal of Gastroenterology 100: 2510-2516.

22.

Wang LH , Fang XC , and Pan GZ ( 2004) Bacillary dysentery as a causative factor of irritable bowel syndrome and its pathogenesis. Gut 53: 1096-1101.

23.

Watanable R. , Brannan CI , Copeland NG , Jenkins NA , and Nagata S. ( 1992) Lymphoproliferation disorder in mice explained by defects in Fas antigen that mediates apoptosis. Nature 356: 314-317.

24.

Watanabe T. , Sakai Y. , Miyawaki S. , and Shimizu A. ( 1991) A molecular genetic linkage map of mouse chromosome 19, including thelpr, Ly-44, andTdt genes. Biochemical Genetics 29: 325-335.

25.

Yarilin A. , Belyakov M. , Kusmenok OI , Arshinov Y. , and Simonova AV ( 1993) Late T cell deficiency in victims of the Chernobyl radiation accident: possible mechanisms of induction. International Journal of Radiation Biology 63: 519-528.

26.

Zhivotovsky B. , Perlaky L. , and Fonagy A. ( 1998) Nuclear protein synthesis in thymocytes of X irradiated rats. International Journal of Radiation Biology 54: 999-1006.