The present study was undertaken to determine the effect of co-exposure to static magnetic field (SMF) and cadmium (Cd) on the antioxidant enzymes activity and DNA integrity in rat brain. Sub-chronic exposure to CdCl (CdCl2, 40 mg/L, per os) for 30 days resulted in a significant reduction in antioxidant enzyme activity such as the glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) in frontal cortex and hippocampus. Total GSH were decreased in the frontal cortex of the Cd-exposed group. Cd exposure induced an increase in malondialdehyde (MDA) concentration in the frontal cortex and hippocampus. Moreover, the same exposure increased 8-oxo-7,8-dihydro-2-desoxyguanosine (8-oxodGuo) level in rat brain. Interestingly, the combined effect of SMF (128 mT, 1 hour/day for 30 consecutive days) and CdCl (40 mg/L, per os) decreased the SOD activity and glutathione level in frontal cortex as compared with the Cd group. Moreover, the association between SMF and Cd increased MDA concentration in frontal cortex as compared with Cd-exposed rats. DNA analysis revealed that SMF exposure failed to alter 8-oxodGuo concentration in Cd-exposed rats. Our data showed that Cd exposure altered the antioxidant enzymes activity and induced oxidative DNA lesions in rat brain. The combined effect of SMF and Cd increased oxidative damage in rat brain as compared with Cd-exposed rats.
Abdelmelek H., Molnar S., Servais S., et al. (2006) Skeletal muscle HSP72 and norepinephrine response to static magnetic field in rat. Journal of Neural Transmission113: 821-827.
2.
Aebi H. ( 1984) Catalase in vitro. Methods in Enzymology105: 121-126.
3.
Agency for Toxic Substances and Disease Registry (ATSDR) ( 1999) Toxicological Profile for Cadmium. US Department of Human and Health Services.
4.
Akerboom TPM, Sies H. ( 1981) Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods Enzymology77: 373-382.
5.
Amara S., Abdelmelek H., Garrel C., et al. (2006) Influence of static magnetic field on cadmium toxicity: study of oxidative stress and DNA damage in rat tissues. Journal of Trace Elements and Medicine Biology20(4): 263-269.
6.
Amara S., Abdelmelek H., Garell C., et al. (2006) Effects of sub-chronic exposure to static magnetic field on testicular function in rats. Archives of Medical Research37: 947-952.
7.
Amara S., Douk T., Ravanat JL, et al. (2007) Influence of a static magnetic field (250 mT) on the antioxidant response and DNA integrity in THP1 cells. Physics in Medicine Biology52: 889-898.
8.
Amara S., Abdelmelek H., Garrel C., et al. (2008) Preventive effect of zinc against cadmium-induced oxidative stress in the rat testis. Journal of reproductive development54:129-34.
9.
Andorn AC, Britton RS, and Bacon BR ( 1990) Evidence that lipid peroxidation and total iron are increased in Alzheimer’s brain. Neurobiology Aging11: 316.
10.
Arvidson B., Tjälve H. ( 1986) Distribution of 109Cd in the nervous system of rats after intravenous injection. Acta Neuropathology69: 111-116.
11.
Atesi I., Suzen HS, Aydin A., and Karakaya A. ( 2004) The oxidative DNA base damage in testes of rats after intraperitoneal cadmium injection. Biometals17: 371-377.
12.
Bauer V., Bauer F. ( 1999) Reactive oxygen species as mediators of tissue protection and injury. General Physiology and Biophysics18: 7-14.
13.
Bauer R., Demeter I., Hasemann V., and Johansen JT ( 1980) Structural properties of the zinc site in Cu, Znsuperoxide dismutase; perturbed angular correlation of gamma ray spectroscopy on the Cu, 111Cd-superoxide dismutase derivative. Biochemical and Biophysical Research Communications94: 1296-1302.
14.
Boguszewska A., Pasternak K. ( 2004) Cadmium-influence on biochemical processes of the human organism. Annales Universitatis Mariae Curie-Sklodowska59: 519-523.
15.
Douki T., Court M., Sauvaigo S., Odin F., and Cadet J. ( 2000) Formaton of the main UV-induced thymine dmeric lesions within isolated and DNA as measured by high performance liquid chromatography-tandem mass spectrometry. Journal of Biology and Chemistry275: 11678-11685.
16.
Ebru B., Mesut A. ( 2002) The effect of long-term supplemental dietary cadmium on lipid peroxidation and the antioxidant system in the liver and kidneys of rabbits. The Turkish Journal of Veterinary and Animal Sciences26: 1055-1060.
17.
El-Maraghy SA, Gad MZ, Fahim AT, and Hamdy MA ( 2001) Effect of cadmium and aluminum intake on the antioxidant status and lipid peroxidation in rat tissues. Journal of Biochemical and Molecular Toxicology15: 207-214.
18.
Eybl V., Kotyzovıa D., and Bludovskıa M. (2004) The effect of curcumin on cadmium-induced oxidative damage and trace elements level in the liver of rats and mice. Toxicology Letters151: 79-85.
19.
Galan A., Garcia-Bermejo L., Troyano A., et al. (2001) The role of intracellular oxidation in death induction (apoptosis and necrosis) in human promonocytic cells treated with stress inducers (cadmium, heat, X-rays). European Journal of Cellular Biology80: 312-320.
20.
Gałazyn-Sidorczuk M., Brzóska MM, Jurczuk M., et al. (2009) Oxidative damage to proteins and DNA in rats exposed to cadmium and/or ethanol. Chemico-biological interactions180: 31-38.
21.
Gupta A., Murthy RC, and Chandra SV ( 1993) Neurochemical changes in developing rat brain after pre- and postnatal cadmium exposure. Bulletin of Environmental and Contamination Toxicology14: 428-433.
22.
Gutierrez-Reyes EY, Albores A., and R’ıos C. (1998) Increase of striatal dopamine release by cadmium in nursing rats and its prevention by dexamethasone induced metallothionein. Toxicology131: 145-154.
23.
Hashish AH, El-Missiry MA, Abdelkader HI, and Abou-Saleh RH ( 2008) Assessement of biological changes of continuous whole body exposure to static magnetic field and extremely low frequency electromagnetic fields in mice. Ecotoxicology and Environmental Safety71: 895-902.
24.
Honcharuk H., Bardov VH, Kartysh AP, Iavorovs’yī, Korshun MM, and Verpivs’kyī OM (1995) An experimental study of the mechanism of the combined action on the body of ionizing radiation, pesticides, nitrates and lead and cadmium salts. Lik Sprava6: 7-12.
25.
Hurych J., Mirejovska E., Holusa R., Bubenikova D. ( 1996) Effects of magnetic field exposure on the development of lung fibrosis elicited by industrial pollutants. Toxicology Letters88: 305-311.
26.
Jolliet-Riant P., Tillement JP ( 1999) Drug transference across the blood-brain barrier and improvement of brain delivery. Fundamentals in Clinical Pharmacology13: 16-26.
27.
Jones DP, Carlson JL, Mody VC, Cai J., Lynn MJ, and Sternberg P. ( 2000) Redox state of glutathione in human plasma. Free Radicals in Biology and Medicine28: 625-635.
28.
Karasek M., Lerchl A. ( 2002) Melatonin and magnetic fields. Neuroendocrinology Letters23: 84-87.
29.
Kasai H. ( 2002) Analysis of a form of oxidative DNA damage, 8-hydroxy-2’-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutation Research387: 147-163.
30.
Kheifets LI ( 2001) Electric and magnetic field exposure and brain cancer, a review. Biolectromagnetics5: 120-131.
31.
Koterov AN, Trebenok ZA, Pushkareva NB, and Nikol’skī1997) The diverse effects of the combined action of cadmium chloride and ionizing radiation on the content of metallothioneins in mouse bone marrow and liver. Radiatsionnaia biologiia, radioecologiia37: 196-201.
32.
Masella R., Di Benedetto R., Vari R., Filesi C., and Giovannini C. ( 2005) Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes. Journal of Nutrition and Biochemistry16: 577-586.
33.
National Institute of Occupational Safety and Health ( 1990) Testimony on Occupational Exposure to Cadmium.
34.
Okano H. ( 2008) Effects of static magnetic fields in biology: role of free radicals. Frontier in Bioscience13: 6106-6125.
35.
Ozturk A., Baltaci AK, Mogulkoc R., and Oztekin E. ( 2003) Zinc prevention of electromagnetically induced damage to rat testicule and kidney tissues. Biology Trace Elements Research96: 247-257.
36.
Paolotti F., Mocali A. ( 1990) Determination of superoxide dismutase activity by purely chemical system based on NADPH oxidation. Methods Enzymology186: 209-226.
37.
Placer Z., Cushman LL, and Johnson BC ( 1966) Estimation of product of lipid peroxidation (Malonyl Dialdehyde) in biochemical systems. Analytical Biochemistry16: 359-364.
38.
Puget K., Michelson AM ( 1977) Comparative study of superoxide dismutase, catalase and glutathione peroxidase levels in erythrocytes of different animals. Biochemical and Biophysical Research Communications15: 1525-1535.
39.
Ravanat JL, Douki T., Duez P., et al. (2002) Cellular background level of 8-oxo-7,8-dihydro-2-deoxyguanosine: an isotope based method to evaluate artefactual oxidation of DNA during its extraction and subsequent work-up. Carcinogenosis23: 1911-1918.
40.
Rollwitz J., Lupke M., and Simko M. ( 2004) Fifty-hertz magnetic fields induce free radical formation in mouse bone marrow-derived promonocytes and macrophages. Biochimica et Biophysica Acta1674: 231-238.
41.
Rosen AD ( 2003) Mechanism of action of moderate-intensity static magnetic fields on biological systems. Cell Biochemistry and Biophysics39(2): 163-173.
42.
Scassellati SG, Moretti M., Villarini M., Fatigoni C., and Pasquini R. ( 2004) Evaluation of genotoxic and/or co-genotoxic effects in cells exposed in vitro to extremely-low frequency electromagnetic fields . Annali d’igiene16: 321-340.
43.
Stohs SJ, Bagchi D. ( 1995) Oxidative mechanism in the toxicity of metal ions . Free Radical in Biology Medicine18, 321-336.
44.
Sun AY, Chen YM ( 1998) Oxidative stress and neurodegenerative disorders . Journal of Biomedical Science5: 401-414.
45.
Tandon SK, Singh S., Prasad S., et al. (2003) Reversal of cadmium induced oxidative stress by chelating agent, antioxidant or their combination in rat. Toxicology Letters145: 211-217.
46.
Viaene MK, Masschelein R., Leenders J., De Groof M., Swerts L., and Roels H. ( 2000) Neurobehavioural effects of occupational exposure to cadmium: a cross sectional epidemiological study. Occupational and Environmental Medicine57: 19-27.
47.
Wartenberg D. ( 2001) Residential EMF exposure and childhood leukemia: metanalysis and population attributable risk. Biolectromagnetics5: 86-104.
48.
Wasowicz W., Gromadzinska J., and Rydzynski K. ( 2001) Blood concentration of essential trace elements and heavy metals in workers exposed to lead and cadmium. International Journal of Occupational Medicine and Environmental Health14: 223-229.
49.
Wolf FI, Torsello A., Tedesco B., et al. (2005) 50-Hz extremely low frequency electromagnetic fields enhance cell proliferation and DNA damage: possible involvement of a redox mechanism. Biochimica et Biophysica Acta1743: 120-129.
50.
Yokus B., Cakir DU, Akday MZ, Sert C., and Mete N. ( 2005) Oxidative DNA damage in rats exposed to extremely low frequency electromagnetic fields. Free Radical Research39: 317-323.
51.
Yoshida S. ( 2001) Re-evaluation of acute neurotoxic effects of Cd2+ on mesencephalic trigeminal neurons of adult rat. Brain Research892: 102-110.
