Water-soluble binary and ternary copper complexes of polyelectrolytes were synthesized, and the toxicity of these complexes was tested in mouse fibroblast cell line (L929) in vitro. Both the binary and ternary complexes were prepared at the ratio of 0.4 mole copper(II) ions per monomer of acrylic acid and 0.5 mole copper(II) ions per monomer of methyl vinyl ether maleic anhydride, furthermore at the ratio of 1 and 2 mole bovine serum albumin per mole of polyacrylic acid and poly(methyl vinyl ether-co-maleic anhydride), respectively. Compared to binary copper(II)–polyelectrolyte complexes, these ternary complexes have been determined to be of least toxicity.
AndrianovAKMarinADeCollibusDP (2011) Microneedles with intrinsic immunoadjuvant properties: microfabrication, progtein stability, and modulated release. Pharmaceutical Research28: 58–65.
2.
CaiqinQYuminDLingXZhanLXiaohaiG (2002) Enzymic preparation of water-soluble chitosan and their antitumor activity. International Journal of Biological Macromolecules31: 111–117.
3.
ChakrabortyAKumarPGhoshKRoyP (2010) Evaluation of Schiff base copper complex compound as potent anticancer molecule with multiple targets of action. European Journal of Pharmacology647: 1–12.
4.
ChangzhengLJiguiWLiufangWMinRNaiyongJJieG (1999) Synthesis, characterization and antitumor activity of copper(II) complex with nicotinamido-4-bis(2-chloroethyl)aminobenzaldimine. Journal of Inorganic Biochemistry73(4): 195–202.
5.
ChuiVWDMokKWNgCYLuongBPMaKK (1996) Removal and recovery of copper (II), chromium (III), and Nickel (II) from solutions using crude shrimp chitin packed in small columns. Environmental International22(4): 463–468.
6.
ClarkKMGlatzCE (1990) In Downstream Processing and Bioseparation Recovery and Purification of Biological Product. Washington: American Chemical Society, Chapter9.
7.
DingNWLinWHSunWLShenZQ (2011) A novel hyperbranched aromatic polyamide containing bithiazole: Synthesis, metal complexation and magnetic properties. Science China-Chemistry54(2): 320–325.
8.
FilenkoADemchenkoMMustafaevaZYoshihitoOMustafaevMI (2001) Fluorescense study of Cu2+-induced interaction between albumin and anionic polyelectrolytes. Biomacromolecules2(1): 270–277.
9.
HuangCChuangYCLiouMR (1996) Adsorption of Cu(II) and Ni(II) by pelletized biopolymer. Journal of Hazardous Materials45: 265–277.
10.
KabanovAV (2004) From synthetic polyelectrolytes to polymer-subunit vaccines. Pure and Applied Chemistry76(9): 1659–1677.
11.
KarahanM (2009) Development of Functional Biopolymer Systems Containing Metal. Istanbul: Yildiz Technical University.
12.
KarahanMMustafaevaZÖzeroğluC (2010) Fluorescence and dynamic light scattering studies for the formation of ternary complexes of polyacrylic acid with bovine serum albumin in the presence of metal ions. The Protein Journal29(5): 336–342.
13.
KarahanMTuğluSMustafaevaZ (2012) Synthesis of microwave-assisted poly(methyl vinyl ether-co-maleic anhydride)-bovine serum albumin bioconjugates. Artificial Cells Blood Substitutes and Biotechnology. Epub ahead of print 30 April 2012. DOI: 10.3109/10731199. 2012. 678942.
14.
LiangFWuCLinHLiTGaoDLiZ. (2003) Copper complex of hydroxyl-substituted triazamacrocyclic ligand and its antitumor activity. Bioorganic and Medical Chemistry Letters13(15): 2469–2472.
15.
MillerML (1978) Polyacrylic acid polymers. Polym Science and Technology1: 445–452.
16.
MustafaevM (2004) Functionally biopolymer systems. Sigma (Journal of Engineering and Natural Sciences)4: 1–200.
17.
MustafaevMISaracAS (1996) Polyelectrolyte Complexes (in immunology) The Polymeric Materials Encyclopedia: Synthesis. Properties and Application. Solomone: CRC Press Inc.
18.
MustafaevMIMustafaevaZBermekEOsadaY (1998) New amphiphilic immunogens by Cu2+-mediated complexes of water-born poly(N-isopropylacrylamide-co-acrylic acid) and bovine serum albumin. Journal of Bioactive and Compatible Polymers13: 33–49.
MustafaevMIOsadaYMatsukataMBasalpACirakogluBBermekE (1996) New amphiphilic immunogens by poly(N-isopropylacrylamide)-modified bovine serum albumin. Polymer Gels and Networks4: 363–372.
21.
MuzzarelliRAAFerreroAPizzoliM (1972) Light-scattering X-ray diffraction, elemental analysis and infrared spectrophotometry characterization of chitosan, a chelating polymer. Talanta19: 1222–1226.
22.
SaracASYavuzOSezerEMustafaevMI (1997) Cu2+ complexes of vinylpyrrolidone-acrylic acid copolymers and ternary complexes with BSA. Polymer News22: 258–261.
23.
ShaikAPKhanMJamilK (2009) Phylogenetic analysis of ALAD and MGP genes related to lead toxicity. Toxicology and Industrial Health25(6): 403–409.
24.
StregeMADubinPLWestJSFlintaCD (2001) In protein purification: from molecular. Biomacromolecules2(2): 428.
25.
TopuzogullariMCimenNSMustafaevaZMustafaevM (2007) Molecular-weight distribution and structural transformation in water-soluble complexes of poly(acrylic acid) and serum albumin. European Polymer Journal43: 2935–2946.
26.
VarmaAJDeshpandeaSVKennedyJF (2004) Metal complexation by chitosan and its derivatives: a review. Carbohydrate Polymers55: 77–93.
27.
WöhrleDTsuchidaECiardelliF (1995) Macromolecule-Metal Complexes. New York: Springer-Verlag.
28.
ZhaoXZJiangTWangLYangHZhangSZhouP (2010) Interaction of curcumin with Zn(II) and Cu(II) ions based on experiment and theoretical calculation. Journal of Molecular Structure1(3): 316–325.
29.
ZhengYYiYQiYWangYZhangWDuM (2006) Preparation of chitosan-copper complexes and their antitumor activity. Bioorganic and Medicinal Chemistry Letters16: 4127–4129.
