Increased oxidative stress and plasma Hsp70 levels among gasoline filling station attendants

Abstract

Objectives:

Methylcyclopentadienyl manganese tricarbonyl (MMT) is an organic derivative of manganese (Mn) and is used as an antiknock agent and octane enhancer in gasoline. In this article, we tested the oxidative stress and heat stress protein (Hsp) 70 levels of gasoline station attendants to explore potential plasma biomarkers. Furthermore, the dose–response relationship was also identified.

Methods:

A total of 144 workers, including 96 petrol fillers and 48 cashiers, participated in the study. Ambient concentrations of benzene, toluene, ethylbenzene, and xylene (BTEX) and Mn were monitored at nine filling stations. During the measuring process, the individual cumulative exposure index was calculated. Plasma oxidative stress and Hsp70 levels were also analysed using enzyme-linked immunosorbent assay.

Results:

The BTEX time-weighted average in office areas was significantly lower than in refuelling areas (p < 0.05). In refuelling areas, the content of Mn ranged from 6.44 μg/m³ to 127.34 μg/m³, which was much higher than that in office areas (3.16–7.22 μg/m³; p < 0.05). Exposed workers had significantly different plasma oxidative stress indicators compared with the control group, respectively: superoxide dismutase (SOD), 39.18 ± 6.05 U/mL versus 52.84 ± 3.87 U/mL; glutathione peroxidase (GSH-Px), 186.07 ± 15.63 U versus 194.38 ± 10.42 U; and malondialdehyde (MDA), 1.68 ± 0.52 nmol/L versus 1.43 ± 0.64 nmol/L (in all comparisons, p < 0.05). Plasma Hsp70 level in the exposed group (2.77 ± 0.64 ng/mL) was significantly higher than in the control group (2.32 ± 0.87 ng/mL; p < 0.05). Furthermore, Hsp70 levels were inversely correlated with the activities of SOD (r = −0.305) and GSH-Px (r = −0.302) in the exposed group (p < 0.05). Moreover, a positive correlation (r = 0.653) was found between plasma Hsp70 levels and plasma MDA levels (p < 0.05).

Conclusion:

Exposure to MMT-containing gasoline may result in increasing reactive oxygen stress among filling station attendants. Plasma Hsp70 levels could be used as a sensitive responsive biomarker for exposed workers.

Keywords

Methylcyclopentadienyl manganese tricarbonyl BTEX manganese oxidative stress heat stress protein 70

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