β-glucuronidase (BG) activity is a promising biomarker for diagnosis and prognosis after exposure to organophosphorous (OP) pesticides. The aim of this study was to evaluate the changes in serum BG activity in patients with acute OP poisoning and to determine whether these changes correlate with the severity of poisoning. Thirty patients with anticholinesterase pesticide poisoning were included, besides 10 healthy volunteers as a control group. Serum activities of butyrylcholinesterase (BuChE) and BG were measured for each subject on admission, then after 12 and 24 h. Serum levels of BuChE and BG in poisoned patients were significantly different from the control subjects; these differences persisted in repeated measurements. Moreover, the serum levels showed significant differences within each group of the three time points. A significant negative correlation was found between the serum activities of BuChE and BG in all groups at the three time points. In conclusion, serum BG activity seems a reliable marker for OP poisoning even when measured at 24 h after poisoning.
BalmeKHRobertsJCGlasstoneM. (2010) Pesticide poisonings at a tertiary children’s hospital in South Africa: an increasing problem. Clinical Toxicology (Philadelphia, Pa.)48(9): 928–934.
2.
BasinskaAFlorianczykB (2003) Beta-glucuronidase in physiology and disease. Annales Universitatis Mariae Curie-Sklodowska. Sectio D: Medicina58(2): 386–389.
3.
BlackRM (2008) An overview of biological markers of exposure to chemical warfare agents. Journal of Analytical Toxicology32(1): 2–9.
4.
BuckleyNAEddlestonMLiY. (2011) Oximes for acute organophosphate pesticide poisoning. Cochrane Database of Systematic Reviews16(2): CD005085.
5.
ChondrogianniNFragoulisEGGonosES (2002) Protein degradation during aging: the lysosome-, the calpain- and the proteasome-dependent cellular proteolytic systems. Biogerontology3(1–2): 121–123.
6.
DawsonBTrappRG (2001) Basic and Clinical Biostatics. Lang Medical Book. 3rd ed. New York: McGrow Hill Medical.
7.
Den BlawenDHPoppeWATrischlerW (1983) Manual of AChE determination kit. Journal of Clinical Chemistry and Clinical Biochemistry21(6): 381–386.
8.
DhotreSNKatkamRVJoshiNG. (2014) Study of acetyl cholinesterase, butyryl cholinestrase and β-glucuronidase in organophosphorus poisoning. Indian Medical Gazette148(2): 51–57.
9.
EyerP (2003) The role of oximes in the management of organophosphorus pesticide poisoning. Toxicological Reviews22: 165–190.
10.
FalkenbachAUnkelbachUGottschalkR. (1991) Serum level of beta-glucuronidase as potential indicator of disease activity in Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE). Medizinische Klinik (Munich)86(9): 465–468.
11.
FujikawaYSatohTSuganumaA. (2005) Extremely sensitive biomarker of acute organophosphorus insecticide exposure. Human and Experimental Toxicology24: 333–336.
12.
GoldsteinG (1961) Serum β-glucuronidase assay by the phenolphthalein mono-β-glucuronide method. Clinical Chemistry7(2): 136–142.
13.
GratzMKunert-KeilCJohnU. (2005) Identification and functional analysis of genetic variants of the human beta-glucuronidase in a German population sample. Pharmacogenet. Genomics15(12): 875–881.
14.
Inayat-HussainSHLubisSHSakianNI. (2007) Is plasma beta-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience. Toxicology and Applied Pharmacology219(2–3): 210–216.
15.
JokanovicM (2009) Current understanding of the mechanisms involved in metabolic detoxification of warfare nerve agents. Toxicology Letters188(1): 1–10.
16.
KaleB (2013) β-glucuronidase as a new potent biomarker in the diagnosis and prognosis of organophosphorus toxicity. International Journal of Biological Research1(1): 5–9.
17.
KarczmarA (1998) Invited review: anticholinesterases: dramatic aspects of their use and misuse. Neurochemistry International32: 401–411.
18.
KhuranaDPrabhakarS (2000) Organophosphorous intoxication. Archives of Neurology57: 600–602.
19.
KikuchiHSuzukiYHashimotoY (1981) Increase of beta-glucuronidase activity in the serum of rats administered organophosphate and carbamate insecticides. The Journal of Toxicological Sciences6: 27–35.
20.
MarutiSSLiLChangJL. (2010) Dietary and demographic correlates of serum beta-glucuronidase activity. Nutrition and Cancer62(2): 208–219.
21.
MintonNAMurrayVS (1988) A review of organophosphate poisoning. Medical Toxicology and Adverse Drug Experience3(5): 350–375.
22.
MremaEMwabulamboSNgowiA. (2017) Pesticide exposure and health problems among female horticultural workers in Tanzania. Environmental Health Insights11: doi: 10.1177/1178630217715237. PMID: 28690397.
23.
NiggHNKnaakJB (2000) Blood cholinesterase as human biomarkers of organophosphorus pesticide exposure. Reviews of Environmental Contamination and Toxicology163: 29–111.
24.
PetroianuGANurulainSMNagelkerkeN. (2007) Five oximes (K-27, K-48, obidoxime, HI-6 and trimedoxime) in comparison with pralidoxime: survival in rats exposed to methyl-paraoxon. Journal of Applied Toxicology27(5): 453–457.
25.
SabbeMBDesruellesDLissensW (2008) Is β-glucuronidase a clinical useful biomarker for an acute organophosphorus poisoning?Human and Experimental Toxicology27: 431–433.
26.
SatohT (1991) Release of liver microsomal betaglucuronidase from hepatocytes in vitro and in vivo by organophosphates and hepatotoxic agents. Journal of Toxicological Sciences16(suppl 1): 133–142.
27.
SatohTSuzukiSKawaiN. (1999) Toxicological significance in the cleavage of esterase-beta-glucuronidase complex in liver microsomes by organophosphorus compounds. Chemico-Biological Interactions119–120: 471–478.
28.
SenarathnaLJayamannaSFKellyPJ. (2012) Changing epidemiologic patterns of deliberate self poisoning in a rural district of Sri Lanka. BMC Public Health12: 593.
29.
SimonianAGoodTWangSS. (2005) Nanoparticle-based optical biosensors for the direct detection of organophosphate chemical warfare agents and pesticides. Analytica Chimica Acta534(1): 69–77.
30.
SoltaninejadKShadniaSAfkhami-TaghipourM. (2007) Blood beta-glucuronidase as a suitable biomarker at acute exposure of severe organophosphorus poisoning in human. Human and Experimental Toxicology26: 963–966.
31.
WongLRadicZBruggemannRJ. (2000) Mechanism of oxime reactivation of acetylcholinesterase analyzed by chirality and mutagenesis. Biochemistry and Cell Biology39: 5750–5757.
32.
World Health Organization (2018) Poisoning prevention and management [Online]. Available at: http://www.who.int/ipcs/poisons/en/(accessed 21 May 2018).
