Effect of Iron Deficiency Anemia On Lead Distribution After Intravenous Dosing in Rats

Abstract

Objective: To determine the effect of iron deficiency anemia on blood and tissue lead distribution.Methods: 24 weanling rats were divided into 2 groups. One group received an iron replete diet (200 ppm); the other received a low iron diet (20 ppm). After 24 days, each group was further subdivided into two doses of lead (5 mg/kg and 10 mg/kg) which was administered intravenously. Rats were continued on their respective diets for 7 days post-lead injection to allow tissue distribtrtion, then sacrificed and blood and tissue lead concentration measured. Results: Prior to lead administration, baseline blood lead concentrations were not significantly different between groups. At sacrifice, whole blood lead levels were significantly higher in iron deficient animals than in iron replete at both 5 and 10 mg/kg administered lead. Iron deficient animals had comparable lead concentrations to iron replete animals in brain, kidney and liver. Femur lead concentrations were higher at 10 mg/kg administered lead. Conclusion: Iron deficiency alters lead distribution such that increased lead is found in blood for a given exposure.

Keywords

hypochromic anemia iron iron deficiency lead lead poisoning.

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References

Aungst, B.J. , Dolce, J.A. , and Fung, H.L. (1981). "The effect of dose on the disposition of lead in rats after intravenous and oral administration:' Toxicol. Appl. Pharmacol. 61:48-57.

Barton, J.C. , Conrad, M.E. , Nuby, S. , and Harrison, S. (1978). "Effects of iron in the absorption and retention of lead." J. Lab. Clin. Med. 92:536-547.

Beard,J.L. , Zhan, C.S. , and Brigham, D.E. (1995). "Growth in iron-deficient rats." Proc. Soc. Exp. Bio. Med. 209:65-72.

Chalevelakis, G. , Bouronikou, H. , Yalouris, A.G. , Economopoulos, T.H. , Athanaselis, S. , and Raptis, S. (1995). "Delta-aminolevulinic acid dehydratase as an index of lead toxicity: Time for a reappraisal?" Eur. J. Clin. Invest . 25:53-58.

Church, H.J. , Day, J.P. , Braithwaite, R.A. , and Brown, S.S. (1993). "Binding of lead to a metallothionein-like protein in human erythrocytes." J. Inorg. Biochem. 49:55-68.

Dallman, PR (1986). "Biochemical basis of iron deficiency." Annu. Rev. Nutr. 6:13-40.

Leggett, R.W. (1993). "An age-specific kinetic model of lead metabolism in humans." Environ. Health Perspect. 101:598-615.

Lolin, Y. and O'Gorman, P. (1988). "An intra-erthrocytic low molecular weight lead binding protein in acute and chronic lead exposure and its possible protective role in lead toxicity." Ann. Clin. Biochem. 25:688-697.

Mahaffey-Six, K. and Goyer, R.A. (1972). "The influence of iron deficiency on tissue content and toxicity of ingested lead in the rat." J. Lab. Clin. Med . 79:128-136.

10.

Morgan, A. , Holwes, A. , and Evans, J.C. (1976). "Retention, distribution and excretion of lead by the rat after intravenous injection." Br. J. Ind. Med. 34:37-42.

11.

Ragan, H.A. (1977). "Effects of iron deficiency on the absorption and distribution of lead and cadmium in rats." J. Lab. Clin. Med. 90:700-706.

12.

Rhagavan, S.R.V. and Gonick, H.C. (1977). "Isolation of low molecular-weight lead binding protein from human erythrocytes." Proc. Soc. Exp. Biol. 155:164-167.