Resveratrol Dietary Supplementation Shortens the Free-Running Circadian Period and Decreases Body Temperature in a Prosimian Primate

Abstract

Resveratrol (RSV) is a dietary polyphenolic compound with several positive effects on metabolic functions and longevity. We tested the effect of RSV on the circadian clock in a nonhuman primate, the gray mouse lemur. The impact of a 2-week dietary supplementation of RSV on the rhythms of locomotor activity and body temperature in constant dark conditions (DD) was investigated in young (n = 7) and old (n = 6) animals. RSV supplementation followed 2 weeks in DD under normal diet (CTL). In both young and old animals receiving RSV, we observed a shortening of the free-running period compared to those under CTL (-15 minutes in young animals and -45 minutes in old animals), accompanied by a lower mean body temperature in both age groups and decreased locomotor activity in young animals. Thus, RSV is a food component capable of influencing a primate’s circadian clock. This property may be of interest clinically in the context of the treatment of circadian disruption and in the context of the effects of RSV ingestion on health.

Keywords

circadian rhythms free-running period Microcebus murinus resveratrol locomotor activity

References

Aujard F. , Cayetanot F. , Bentivoglio M. , and Perret M. ( 2006) Age-related effects on the biological clock and its behavioral output in a primate. Chronobiol Int 23(1-2):451-460.

Baur JA (2010) Resveratrol, sirtuins, and the promise of a DR mimetic. Mech Ageing Dev 131(4):261-269.

Baur JA and Sinclair DA ( 2006) Therapeutic potential of resveratrol: the in vivo evidence . Nat Rev Drug Discov 5(6):493-506.

Cantó C. and Auwerx J. ( 2009) Caloric restriction, SIRT1 and longevity. Trends Endocrinol Metab 20(7):325-331.

Cayetanot F. , Van Someren EJ , Perret M. , and Aujard F. ( 2005) Shortened seasonal photoperiodic cycles accelerate aging of the diurnal and circadian locomotor activity rhythms in a primate. J Biol Rhythms 20(5):461-469.

Challet E. , Solberg LC , and Turek FW ( 1998) Entrainment in calorie-restricted mice: conflicting zeitgebers and free-running conditions. Am J Physiol 274(6Pt2):R1751-R1761.

Das DK , Mukherjee S. , and Ray D. ( 2010) Resveratrol and red wine, healthy heart and longevity . Heart Fail Rev 15(5):467-477.

Génin F. and Perret M. ( 2003) Daily hypothermia in captive gray mouse lemurs (Microcebus murinus): effects of photoperiod and food restriction. Comp Biochem Physiol B Biochem Mol Biol 36(1):71-81.

Lagouge M. , Argmann C. , Gerhart-Hines Z. , Meziane H. , Lerin C. , Daussin F. , Messadeq N. , Milne J. , Lambert P. , Elliott P. , et al. (2006) Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1alpha . Cell 127(6): 1109-1122.

10.

Mayers JR , Iliff BW , and Swoap SJ ( 2009) Resveratrol treatment in mice does not elicit the bradycardia and hypothermia associated with calorie restriction. FASEB J 23(4):1032-1040.

11.

Mendoza J. ( 2007) Circadian clocks: setting time by food. J Neuroendocrinol 19(2):127-137.

12.

Nakahata Y. , Kaluzova M. , Grimaldi B. , Sahar S. , Hirayama J. , Chen D. , Guarente LP , and Sassone-Corsi P. (2008) The NAD+-dependent deacetylase SIRT1 modulates CLOCK-mediated chromatin remodeling and circadian control . Cell 134(2):329-340.

13.

Oike H. and Kobori M. ( 2008) Resveratrol regulates circadian clock genes in Rat-1 fibroblast cells. Biosci Biotechnol Biochem 72(11):3038-3040.

14.

Sun AY , Wang Q. , Simonyi A. , and Sun GY ( 2010) Resveratrol as a therapeutic agent for neurodegenerative diseases. Mol Neurobiol 41(2-3):375-383.

15.

Terrien J. , Ambid L. , Nibbelink M. , Saint-Charles A. , and Aujard F. ( 2010) Non-shivering thermogenesis activation and maintenance in the aging gray mouse lemur (Microcebus murinus). Exp Gerontol 45(6): 442-448.

16.

Xia EQ , Deng GF , Guo YJ , and Li HB ( 2010) Biological activities of polyphenols from grapes. Int J Mol Sci 11(2):622-646.

17.

Zhang F. , Liu J. , and Shi JS ( 2010) Anti-inflammatory activities of resveratrol in the brain: role of resveratrol in microglial activation. Eur J Pharmacol 636(1-3):1-7.