In Vitro Evaluation of 3T3 and MDBK Cells Attachment and Proliferation on Collagen and Fibronectin Immobilized Nonwoven Polylactide Matrices

Abstract

Fibroblast-like, 3T3 (Mouse Swiss Albino) and epithelial-like MDBK (Madine Darby Bovine Kidney) cells were selected as model cell lines and the adhesion of these cells on nonwoven poly DL-lactide (PDLLA) matrices were investigated. PDLLA was synthesized by ring-opening polymerization of DL-lactide. Nonwoven PDLLA matrices were prepared by an extrusion/winding process. Surface modification of these matrices was achieved by glow-discharge treatment, which was followed by glutaraldehyde incorporation. Biologically modification was performed by immobilization of collagen and/or fibronectin for cell attachment and proliferation. Adhesion values of fibroblast-like and epithelial-like cells after incubation for 2 h was 90 and 85% of initial cells, respectively. The 3T3 cells grown on PDLLA, after 11 days of incubation, was 9 10⁵ cells while the control group was 1.2 10⁶ cells. MDBK cells grown on the same polymeric matrices were determined to be 1.5 10⁶ cells and 1.8 10⁶cells for the control group. These results indicate that biodegradable nonwoven PDLLA matrices can be used for adhesion and proliferation of primary cell cultures and that implants of polymer-cells composites can be used in wound healing. An important advantage of these matrices is that a second surgical operation is not necessary to remove the implant due to the biodegradation of polymer in tissue.

Keywords

PDLLA fibers surface modification glow-discharge collagen immobilization fibronectin immobilization cell attachment cell proliferation

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