pH-Sensitive Hydrogels Composed of Chitosan and Polyacrylamide: Enzymatic Degradation

Abstract

The enzymatic degradation of three types of pH-sensitive hydrogels, composed of the natural polyaminosaccharide chitosan and polyacrylamide, was studied. The weight of the films that were made with net-PAAm- i-chitosan, net-chitosan- i-PAAm and net-chitosan- net-PAAm decreased in the presence of the Trichoderma viride enzyme complex; thus, the chitosan in the composite retained its degradability after crosslinking. The rate of enzymatic degradation depended on the structure of the network, on the amount of crosslinking agents, on the pH of the medium and on the temperature. Crosslinked chitosan alone degraded slower than net-chitosan- i-PAAm; this was attributed to the facilitated penetration of enzyme by the water-soluble PAAm in the semi-IPNs. T. viride embedded in chitosan/PAAm films or beads developed and reproduced normally. However, T. viride embedded in net-chitosani-PAAm developed considerably slower, and development was not detected in the case of net-PAAm- i-chitosan. All of the networks proved to be appropriate carriers of Bacillus subtilis.

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