Plasma-activated fibrinogen coatings onto poly(vinylidene fluoride) surface for improving biocompatibility with tissues

CO₂, N₂, and N₂/H₂ radiofrequency plasma exposure was used for functionalization of poly(vinylidene fluoride) surface aiming the fibrinogen immobilization. Fibrinogen was immobilized onto poly(vinylidene fluoride) surface using both simple plasma activation and covalent coupling. The modified surfaces have been characterized by X-ray photoelectron spectroscopy, attenuated total reflectance–Fourier transform infrared spectroscopy, near infrared–chemical imaging, atomic force microscopy, and wettability measurements, and the obtained materials were tested as supports for fibroblast cell cultures. The plasma type and the immobilization procedure have influenced the fibrinogen attachment onto the poly(vinylidene fluoride) surface, which was achieved mainly through amide bonds when using coupling agents. Covalent immobilization of fibrinogen onto poly(vinylidene fluoride) surface leads to a more stable protein-modified polymer surface. Non-cytotoxic plasma-based coating technology has the ability to covalently immobilize bioactive molecules for surface modification of some biomaterials that mainly could be achieved by the immobilization of proteins such as fibrinogen that triggers desirable cellular responses. The fibrinogen-modified poly(vinylidene fluoride) materials showed increased cell viability of fibroblasts. Cell viability was enhanced by plasma-activated fibrinogen coatings onto poly(vinylidene fluoride) surface, this being more significant if coating was linked further by a coupling reaction. Hence, they could be good candidates for biomedical applications.