An injectable hydrogel was obtained from the high methyl-esterified plum Prunus domestica L. (PD) pectin and calcium ions (Ca2+). PD hydrogel showed a weak gel-like behavior and could be squeezed out of the syringe with an injection force of ca. 9 N. PD hydrogel was not suitable for the NIH/3T3 fibroblast cell adhesion in vitro. The live/dead fluorescence and MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide) assays indicated that the PD hydrogel had a low cytotoxicity in relation to both the adhered and gel surrounding fibroblasts. PD hydrogel was found to inhibit adhesion formation in the sidewall defect-cecum abrasion rat model. In the control group, the occurrence of adhesion of the cecum to the peritoneal wall was found in seven of the total seven rats operated. Only four of the seven animals that were treated with the PD hydrogel were noted to have any adhesions. These adhesions were of a minimum grade of 1, 2, and 3 and were represented by a thin film that could be easily broken. The protective effect of PD hydrogel was found to be comparable with that of hyaluronic acid hydrogel used as a positive control. PD hydrogel appeared to possess enhanced in vivo residence stability on the injury sites compared to hyaluronic acid hydrogel as measured by staining of healing tissue with periodic acid-Schiff reagent. The data obtained offered the prospect for the development of the pectin-based gels as new barrier materials for surgery.
BruggmannDTchartchianGWallwienerM. Intra-abdominal adhesions: definition, origin, significance in surgical practice, and treatment options. Dtsch Arztebl Int2010; 107: 769–775.
2.
BroekRPGStommelMWJStrikC. Benefits and harms of adhesion barriers for abdominal surgery: a systematic review and meta-analysis. Lancet2014; 383: 48–59.
3.
WardBCPanitchAP.Abdominal adhesions: current and novel therapies. J Surg Res2011; 165: 91–111.
4.
BrochhausenCSchmittVHRajabTK. Intraperitoneal adhesions—an ongoing challenge between biomedical engineering and the life sciences. J Biomed Mater Res A2011; 98(1): 143–156.
LauderCIWGarceaGStricklandA. Abdominal adhesion prevention: still a sticky subject?Dig Surg2010; 27: 347–358.
7.
ChuDILimRHeydrickS. N-acetyl-L-cysteine decreases intra-abdominal adhesion formation through the upregulation of peritoneal fibrinolytic activity and antioxidant defenses. Surgery2011; 149: 801–812.
8.
ParsaeiPKarimiMAsadiSY. Bioactive components and preventive effect of green tea (Camellia sinensis) extract on post-laparotomy intra-abdominal adhesion in rats. Int J Surg2013; 11: 811–815.
9.
SahbazAAyniogluOIsikH. Bromelain: a natural proteolytic for intra-abdominal adhesion prevention. Int J Surg2015; 14: 7–11.
10.
ItoTFraserIPYeoY. Anti-inflammatory function of an in situ cross-linkable conjugate hydrogel of hyaluronic acid and dexamethasone. Biomaterials2007; 28: 1778–1786.
11.
YeoYAdilMBellasE. Prevention of peritoneal adhesions with an in situ cross-linkable hyaluronan hydrogel delivering budesonide. J Control Release2007; 120: 178–185.
12.
CaffallKHMohnenD.The structure, function, and biosynthesis of plant cell wall. Carbohydr Res2009; 344: 1879–1900.
13.
RoundANRigbyNMMacDougallAJ. A new view of pectin structure revealed by acid hydrolysis and atomic force microscopy. Carbohydr Res2010; 345: 487–497.
14.
FraeyeIDuvetterTDounglaE. Fine-tuning the properties of pectin–calcium gels by control of pectin fine structure, gel composition and environmental conditions. Trends Food Sci Technol2010; 21: 219–228.
15.
ChenCHSheuMTChenTF. Suppression of endotoxin-induced proinflammatory responses by citrus pectin through blocking LPS signaling pathways. Biochem Pharmacol2006; 72: 1001–1009.
16.
ChanputWMesJVreeburgRAM. Transcription profiles of LPS-stimulated THP-1 monocytes and macrophages: a tool to study inflammation modulating effects of food-derived compounds. Food Funct2010; 1: 254–261.
17.
ChenLLiuJZhangY. Structural, thermal, and anti-inflammatory properties of a novel pectic polysaccharide from alfalfa (Medicago sativa L.) stem. J Agric Food Chem2015; 63: 3219–3228.
18.
GalletMVayssadeMMorraM. Inhibition of LPS-induced proinflammatory responses of J774.2 macrophages by immobilized enzymatically tailored pectins. Acta Biomater2009; 5: 2618–2622.
19.
SalmanHBergmanMDjaldettiM. Citrus pectin affects cytokine production by human peripheral blood mononuclear cells. Biomed Pharmacother2008; 62: 579–582.
20.
PopovSVOvodovaRGPopovaGY. Adhesion of human neutrophils to fibronectin is inhibited by comaruman, pectin of marsh cinquefoil Comarum palustre L., and by its fragments. Biochemistry2005; 70: 108–112.
21.
PopovSVOvodovaRGPopovaGY. Inhibition of neutrophil adhesion by pectic galacturonans. Bioorg Khim2007; 33: 175–180.
22.
DalonsoNPetkowiczCL.Guarana powder polysaccharides: characterisation and evaluation of the antioxidant activity of a pectic fraction. Food Chem2012; 134: 1804–1812.
23.
WangXLuX.Characterization of pectic polysaccharides extracted from apple pomace by hot-compressed water. Carbohydr Polym2014; 102: 174–184.
24.
PandaBMondalSDeviKSP. Pectic polysaccharide from the green fruits of Momordica charantia (Karela): structural characterization and study of immunoenhancing and antioxidant properties. Carbohydr Res2015; 401: 24–31.
25.
PengHHChenJWYangTP. Polygalacturonic acid hydrogel with short-chain hyaluronate cross-linker to prevent postoperative adhesion. J Bioact Compat Polym2011; 26: 552–564.
26.
PopovSVOvodovaRGGolovchenkoVV. Pectic polysaccharides of the fresh plum Prunus domestica L. isolated with a simulated gastric fluid and their anti-inflammatory and antioxidant activities. Food Chem2014; 143: 106–113.
27.
LiLWangNJinX. Biodegradable and injectable in situ cross-linking chitosan-hyaluronic acid based hydrogels for postoperative adhesion prevention. Biomaterials2014; 35: 3903–3917.
28.
ScottRW.Colorimetric determination of hexuronic acids in plant materials. Anal Chem1979; 51: 936–941.
29.
NaSYOhSHSongKS. Hyaluronic acid/mildly crosslinked alginate hydrogel as an injectable tissue adhesion barrier. J Mater Sci Mater Med2012; 23: 2303–2313.
30.
WeisCOdermattEK.A-part gel—an efficient adhesion prevention barrier. J Biomed Mater Res B Appl Biomater2007; 82: 174–182.
31.
MunarinFPetriniPBarcellonaG. Reactive hydroxyapatite fillers for pectin biocomposites. Mater Sci Eng C Mater Biol Appl2014; 45: 154–161.
32.
O’BrienAPhilpKMorrisE.Gelation of high-methoxy pectin by enzymic de-esterification in the presence of calcium ions: a preliminary evaluation. Carbohydr Res2009; 344: 1818–1823.
33.
NgouemazongDETengwehFFFraeyeI. Effect of de-methylesterification on network development and nature of Ca2+-pectin gels: towards understanding structure–function relations of pectin. Food Hydrocoll2012; 26: 89–98.
34.
NgouemazongDEJolieRPCardinaelsR. Stiffness of Ca2+-pectin gels: combined effects of degree and pattern of methylesterification for various Ca2+ concentrations. Carbohydr Res2012; 348: 69–76.
35.
TakeiTSugiharaKIjimaH. In situ gellable sugar beet pectin via enzyme-catalyzed coupling reaction of feruloyl groups for biomedical applications. J Biosci Bioeng2011; 112: 491–494.
36.
ChangCWangZCQuanCY. Fabrication of a novel pH-sensitive glutaraldehyde cross-linked pectin nanogel for drug delivery. J Biomater Sci Polymer Ed2007; 18: 1591–1599.
37.
KumarPTSRamyaCJayakumarR. Drug delivery and tissue engineering applications of biocompatible pectin–chitin/nano CaCO3 composite scaffolds. Colloids Surf B Biointerfaces2013; 106: 109–116.
MoreiraHRMunarinFGentiliniR. Injectable pectin hydrogels produced by internal gelation: pH dependence of gelling and rheological properties. Carbohydr Polym2014; 103: 339–347.
