Improved proliferation and osteogenic differentiation of human mesenchymal stem cells on a titanium biomaterial grafted with poly(sodium styrene sulphonate) and coated with a platelet-rich plasma proteins biofilm

Abstract

In order to replace damaged or lost bone in the human body, it is necessary to produce ‘spare body parts’ which are dependent on the use of biomaterial and stem cells and are referred to as ‘tissue engineering’. Surface modification and stem cell interaction of orthopaedic implants offer a promising approach and are investigated here specifically to improve osseointegration of the biomaterial. Osseointegration of titanium implants used in orthopaedic surgery requires that osseo-progenitor cells attach and adhere to the surface, proliferate, then differentiate into osteoblasts and, finally, produce a mineralised matrix. The surface modification of titanium with anionic polymer combined with coating of platelet-rich plasma is provided to create a favourable environment to promote early and strong fixation of implants. The ability of progenitor cells to attach to the surface during early stages is important in the development of new tissue structures; therefore, we developed in our laboratory a strategy involving the grafting of titanium implants with a polymer of sodium styrene sulphonate (poly(sodium styrene sulphonate)) and a biofilm coating of platelet-rich plasma which enables human mesenchymal stem cell interactions. The resulting biomaterial, titanium-poly(sodium styrene sulphonate) and coating of platelet-rich plasma, Ti-poly(sodium styrene sulphonate)–platelet-rich plasma was developed in order to further improve the biomaterial. In this work, we studied and characterised the ‘in vitro’ response of human mesenchymal stem cells to titanium biomaterial grafted with poly(sodium styrene sulphonate) bioactive polymer and coated with platelet-rich plasma proteins (Ti-poly(sodium styrene sulphonate)–platelet-rich plasma). This study shows an increased cell proliferation with Ti-poly(sodium styrene sulphonate)–platelet-rich plasma compared to foetal calf serum and an enhancement of the Ti-poly(sodium styrene sulphonate)–platelet-rich plasma effects on osteoblast differentiation. The results suggest that Ti-poly(sodium styrene sulphonate)–platelet-rich plasma would be a suitable scaffold for bone tissue engineering.

Keywords

Titanium biomaterial poly(sodium styrene sulphonate)bio-activated scaffold osteogenic differentiation osteogenic proliferation human mesenchymal stem cells platelet-rich plasma

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