The single and synergistic effects of montmorillonite and curcumin-loaded chitosan microparticles incorporated onto poly(lactic acid) electrospun film on wound-healing

Abstract

In this study, the single and synergistic effects of montmorillonite and curcumin-loaded chitosan microparticles incorporated onto the polylactic acid films were investigated on full-thickness excisional wounds of rats . The polylactic acid microfibrillated film was electrospun from a 10% (w/v) polylactic acid in chloroform solution. The curcumin-loaded chitosan microparticles were prepared via ionic gelation technique with high drug loading and encapsulation efficiency. The alkaline-treated polylactic acid films were assembled with positively charged chitosan as the first layer and the negatively charged montmorillonite (NC dressing), curcumin-loaded chitosan microparticles (CCM dressing), and their mixture (1:1 (v/v); NC/CCM dressing) as the second layer via layer-by-layer self-assembly technique. The montmorillonite incorporation increased the hydrophobicity and water vapor transmission rate of the CCM dressing but decreased its water-uptake capacity. The films showed good cytocompatibility to L929 fibroblastic cell line, and the in vivo wound-closure percentage result showed that CCM > NC > NC/CCM > sterile gauze. The overall result indicated that the montmorillonite and curcumin-loaded chitosan microparticles had no considerable positive synergistic effects onwound-healing.

Keywords

Montmorillonite curcumin chitosan microparticle polylactic acid layer-by-layer self-assembly skin wound-dressing

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