Antithrombogenic properties of Tulbaghia violacea– loaded polycaprolactone nanofibers

A broad range of polymers have been utilized for the development of blood-contacting implantable medical devices; however, their rate of failure has raised the need for developing more hemocompatible biomaterial surfaces. In this study, a novel scaffold based on polycaprolactone incorporated with 10% and 15% (w/w) Tulbaghia violacea plant extracts were fabricated using electrospinning technique. The fabricated scaffolds were then treated with T. violacea aqueous plant extracts (100 and 1000 µg/mL) to investigate their use as interfaces for blood-contacting implants. The 10% Tvio scaffold produced the lowest mean fibre diameter (193 ± 30 nm), whereas the 15% Tvio scaffold produces the highest mean fibre diameter (538 ± 236 nm) when compared with the control polycaprolactone (275 ± 61 nm) scaffold. The number of adhered platelets was directly linked to fibre diameter and concentration of plant extract in such a way that the lowest fibre diameter scaffold (10% Tvio) inhibited platelet adhesion, whereas more platelets adhered to the scaffold with the highest fibre diameter (15% Tvio scaffolds). There was also an increase in platelet adhesion as the concentration of T. violacea was increased from 100 to 1000 µg/mL for all designed scaffolds. The improved blood compatibility demonstrated by the 10% Tvio scaffold suggests that the plant possesses antithrombogenic properties, particularly at lower concentrations.