This study involves the preparation, microstructural, physical, mechanical, and biological characterization of novel gelatine and gelatine/elastin gels for their use in the tissue engineering of vascular grafts. Gelatine and gelatine/elastin nanocomposite gels were prepared via a sol-gel process, using soluble gelatine. Gelatine was subsequently cross-linked by leaving the gels in 1% glutaraldehyde. The cross-linking time was optimized by assessing the mass loss of the cross-linked gels in water and examining their mechanical properties in dynamic mechanical tests. Atomic force microscopy (AFM) studies revealed elastin nanodomains, homogeneously distributed and embedded in a bed of gelatine nanofibrils in the 30/70 elastin/gelatine gel. It was concluded that the manufactured nanocomposite gels resembled natural arteries in terms of microstructure and stiffness. The biological characterization involved the culture of rat smooth muscle cells (SMCs) on tubular gelatine and gelatine/ elastin nanocomposite gels, and measurements of the scaffold diameter and the cell density as a function of time.
Sayers, R.D. , Raptis, S., Berce, M. and Miller, J.H.Long-term Results of Femorotibial Bypass with Vein or Polytetrafluoroethylene, Br. J. Surg., 1998: 85: 934-938.
2.
Boss, G.W., Poot, A.A., Geugeling, T., van Aken, W.G. and Feijen, Small-Diameter Vascular Graft Prostheses: Current Status, J. Arch. Physiol. Biochem., 1998: 106: 100.
3.
Stranders, D.E. and Summer, D.S. ( 1975). Grafts and Grafting, In: Hemodynamics for Surgeons, New York, Grune and Stratton, p. 342.
4.
Strobel, R., Bootje, A.H. and van den Dungen, J.J.A.M.Aneurysm Fonnation in Modified Umbilical Vein Grafts, Vasc. Endovasc. Surg., 1996: 11: 417-420.
5.
Brems, J., Casteneda, M. and Garvin, P.J.A Five-year Experience with the Bovine Heterograft for Vascular Access, Arch. Surg., 1986: 121: 941.
6.
Garvin, P.J. and Codd, J.E.Aggressive Approach to Late Thrombosis of Bovine Heterografts, Ann. Surg., 1979: 190: 743-745.
Hastings, G.W. ( 1992). Cardiovascular Biomaterials, London , Springer.
9.
Levick, J.R. ( 2000). An Introduction to Cardiovascular Physiology, 3rd edn, Oxford, Oxford University Press.
10.
Bank, A.J., Wilson, R.F., Kubo, S.H., Holte, J.E., Dresing, T.J. and Wang, H.Direct Effects of Smooth-Muscle Relaxation and Contraction on In-Vivo Human Brachial-Artery Elastic Properties, Circ. Res., 1995: 77: 1008-1016.
11.
Heureux, N.L’. , Stoclet, J-C., Auger, F.A., Lagaud, G.J-L. , Germain, L. and Andriantsitohaina , R.A Human Tissue-Engineered Vascular Media: A New Model for Pharmacological Studies of Contractile Responses , The FASEB J., 2001: 15: 515-524.
12.
Zulliger, M.A. , Rachev, A. and Stergiopoulos , N.A Constitutive Formulation of Arterial Mechanics Including Vascular Smooth Muscle Tone, Am. J. Physiol. Heart Circ. Physiol., 2004: 287: H1335-H1343.
13.
O’Rourke, M.F. ( 1982). Arterial Function in Health and Disease, New York, Churchill Livingstone.
14.
Solomon, E.P. ( 1995). Human Anatomy and Physiology, 2nd edn, London, PA, W.B. Saunders & Co.
15.
Marieb, E.N. ( 1995). Human Anatomy and Physiology, 3rd edn, The Benjamin/Cummings series in the life sciences, New York, Benjamin Cummings publishing company.
16.
Tortora, G.J. and Grabowski, S.R. (1996). Principles of Anatomy and Physiology, 8th edn, New York, Harper Collins College.
17.
Ilona, B.D. ( 1996). Structure and Function of Elastin and Collagen, Budapest, Akademiaai Kiado.
18.
Weinberg, C.B. and Bell, E.A Blood-Vessel Model Constructed from Collagen and Cultured Vascular Cells, Science, 1986 : 231: 397-400.
19.
L’Heureux, N., Paquet, S., Labbe, R., Germain, L. and Auger, F.A.A Completely Biological Tissue-Engineered Human Blood Vessel, FASEB J., 1998: 12: 47-56.
20.
Brinkman, W.T. , Nakapudi, K., Thomas, B.S. and Chaikof, E.L.Photo-Cross-Linking of Type I Collagen Gels in the Presence of Smooth Muscle Cells: Mechanical Properties, Cell Viability, and Function, Biomacromolecules, 2003: 4: 890-895.
21.
Cummings, C.L. , Gawlitta, D., Nerem, R.M. and Stegemann , J.P.Properties of Engineered Vascular Constructs made from Collagen, Fibrin, and Collagen-Fibrin Mixtures , Biomaterials, 2004: 25: 3699-3706.
22.
Baguneid, M. , Murray, D., Salacinski, H.J. et al. Shear-Stress Preconditioning and Tissue-Engineering-Based Paradigms for Generating Arterial Substitutes, Biotech. Appl. Biochem., 2004: 39: 151-157.
23.
Boccafoschi, F., Habermehl, J., Vesentini, S. and Mantovani, D.Biological Performances of Collagen-Based Scaffolds or Vascular Tissue Engineering, Biomaterials, 2005: 26: 7410-7417.
24.
Torikai, K. , Takano, H., Ishikawa, H. et al. Three Layered Biodegradable Vascular Graft with Collagen Microsponge Promotes In Situ Cellularization with Autologous Progenitor Cells for Aortic Surgery, Circulation , 2000: 112: U71.
25.
Mironov, V. , Kasyanov, V., Shu, X.Z. et al. Fabrication of Tubular Tissue Constructs by Centrifugal Casting of Cells Suspended in an In Situ Crosslinkable Hyaluronan-Gelatin Hydrogel, Biomaterials, 2005: 26: 7628-7635.
26.
Daamen, W.F., van Moerkerk, H.T.B., Hafmans, T. et al. Preparation and Evaluation of Molecularly-Defined Collagen-Elastin-Glycosaminoglycan Scaffolds for Tissue Engineering, Biomaterials, 2003: 24: 4001-4009.
27.
Williamson, M.R. , Woollard, K.J., Griffiths, H.R. and Coombes, A.G.A.Gravity Spun Polycaprolactone Fibers for Applications in Vascular Tissue Engineering: Proliferation and Function of Human Vascular Endothelial Cells, Tissue Eng., 2006: 12: 45-51.
28.
Buijtenhuijs, P., Buttafoco, L., Poot, A.A. et al. Tissue Engineering of Blood Vessels: Characterization of Smooth-Muscle Cells for Culturing on Collagen-and-Elastin-Based Scaffolds, Biotech. Appl. Biochem., 2004: 29: 141-149.
29.
Lekakou, C., Lamprou, D., Vidyarthy, U., Karopoulou, E. and Zhdan, P.Structural Hierarchy of Biomimetic Materials for Tissue Engineered Vascular and Orthopedic Grafts, J. Biomed. Mat. Res., B: Appl. Biomat. , 2008: 85B: 461-468.
30.
Freshney, R.L. ( 2005). Culture of Animal Cells - A Manual of Basic Techniques , 5th edn, New York, John Wiley & Sons, pp. 115-128, 335-339.
