Hydroxyapatite nanorods: Soft-template synthesis,characterization and preliminary in vitro tests

Abstract

Synthetic hydroxyapatite nanorods are excellent candidates for bone tissue engineering applications. In this study, hydroxyapatite nanorods resembling bone minerals were produced by using soft-template method with cetyltrimethylammonium bromide. Composite hydroxyapatite/poly(D, L)lactic acid films were prepared to evaluate the prepared hydroxyapatite nanorods in terms of cell affinity. Preliminary in vitro experiments showed that aspect ratio and film surface roughness play a vital role in controlling adhesion and proliferation of human osteoblast cell line MG 63. The hydroxyapatite nanorods with aspect ratios in the range of 5.94–7 were found to possess distinctive properties, with the corresponding hydroxyapatite/poly(D, L)lactic acid films promoting cellular confluence and a fast formation of collagen fibers as early as after 7 days of culture.

Keywords

Nanorods hydroxyapatite CTAB cell adhesion cell affinity

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References

Onuma

. Recent research on pseudobiological hydroxyapatite crystal growth and phase transition mechanisms. Prog Cryst Growth Charact Mater 2006; 52: 223–245.

Burg

KJL

Porter

Kellam

. Biomaterial development for bone tissue engineering. Biomaterials 2000; 21: 2347–2359.

Dash

Cudworth

. Therapeutic applications of implantable drug delivery system. J Pharmacol Toxicol Methods 1998; 40: 1–12.

Suchanek

Yoshimura

. Processing and properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants. J Mater Res 1998; 13: 94–117.

Jiang

Zhang

. Calcium phosphate with well controlled nanostructure for tissue engineering. Curr Appl Phys 2009; 9: S252–S252.

Sadjadi

Meskinfam

Sadeghi

. In situ biomimetic synthesis, characterization and in vitro investigation of bone-like nanohydroxyapatite in starch matrix. Mater Chem Phys 2010; 124: 217–222.

Zandi

Mirzadeh

Mayer

. Biocompatibility evaluation of nano-rod hydroxyapatite/gelatin coated with nano-HAp as a novel scaffold using mesenchymal stem cells. J Biomed Mater Res A 2010; 92(4): 1244–1255.

Ramachandra

Roopa

Kannan

. Solid state synthesis and thermal stability of HAP and HAP – β-TCP composite ceramic powders. J Mater Sci Mater Med 1997; 8: 511–511.

Feng

Musen

Yupeng

. A simple sol-gel technique for preparing hydroxyapatite nanopowders. Mater Lett 2005; 59: 916–919.

10.

Anee

Ashok

Palanichamy

. A novel technique to synthesize hydroxyapatite at low temperature. Mater Chem Phys 2003; 80: 725–725.

11.

Liu

Yang

Troczynski

. Structural evolution of sol-gel-derived hydroxyapatite. Biomaterials 2002; 23: 1679–1679.

12.

Sarig

Kahana

. Rapid formation of nanocrystalline apatite. J Cryst Growth 2002; 237: 55–55.

13.

Ioku

Yamauchi

Fujimori

. Hydrothermal preparation of fibrous apatite and apatite sheet. Solid State Ion 2002; 151: 147–150.

14.

Sujaridworakun

Kon

Fujiwara

. Hydrothermal conversion of calcite crystal to hydroxyapatite. Mater Sci Eng C 2004; 24: 521–525.

15.

Kalita

Verma

. Nanocrystalline hydroxyapatite bioceramic using microwave radiation: synthesis and characterization. Mater Sci Eng C 2010; 30: 295–303.

16.

Meejoo

Maneeprakorn

Winotai

. Phase and thermal stability of nanocrystalline hydroxyapatite prepared via microwave heating. Thermochim Acta 2006; 447: 115–120.

17.

Cho

Kang

. Nano-sized hydroxyapatite powders prepared by flame spay pyrolysis. J Alloy Comp 2008; 464: 282–287.

18.

Lim

Wang

. Processing of hydroxyapatite via microemulsion and emulsion routes. Biomaterials 1997; 18: 1433–1433.

19.

Bose

Saha

. Synthesis and characterization of hydroxyapatite nanopowders by emulsion technique. Chem Mater 2003; 15: 4464–4469.

20.

Liu

Troczyski

Tseng

. Aging effect on the phase evolution of water-based sol-gel hydroxyapatite. Biomaterials 2002; 23: 1227–1236.

21.

Lin

Chang

Cheng

. Hydrothermal microemulsion synthesis of stoichiometric single crystal hydroxyapatite nanorods with mono-dispersion and narrow-size distribution. Mater Lett 2007; 61: 1683–1687.

22.

Wang

Liu

Yin

. Size-controlled synthesis of hydroxyapatite nanorods by chemical precipitation in the presence of organic modifiers. Mater Sci Eng C 2007; 27: 865–869.

23.

Yan

Deng

. Surfactant-assisted hydrothermal synthesis of hydroxyapatite nanorods. Inter J Inorg Mater 2001; 3: 633–633.

24.

Liu

Hou

Wang

. A simple wet chemical synthesis and characterization of hydroxyapatite nanorods. Mater Chem Phys 2004; 86: 69–69.

25.

Banerjee

Bandyopadhyay

Bose

. Hydroxyapatite nanopowders: synthesis, densification and cell-materials interaction. Mater Sci Eng C 2007; 27: 729–735.

26.

SFY

Mark

Kricka

. Polymeric nanotubes and nanorods for biomedical applications. Curr Nanosci 2009; 5: 182–188.

27.

Armentano

Dottori

Fortuati

. Biodegradable polymer matrix nanocomposites for tissue engineering: a review. Polym Degrad Stab 2010; 95: 2126–2146.

28.

Caglioti

Paoletti

Ricci

. Choice of collimators for a crystal spectrometer for neutron diffraction. Nucl Instrum Methods 1958; 35: 223–228.

29.

Leoni

Scardi

Langford

. Characterization of standard reference materials for obtaining instrumental line profiles. Powder Diffr 1998; 13(4): 210–215.

30.

Young

. The Rietveld method. Oxford: Oxford University Press1991.

31.

Leoni

Confente

Scardi

. PM2K: a flexible program implementing whole powder pattern modelling. Z Kristallogr Suppl 2006; 23: 249–254.

32.

Scardi

Leoni

. Whole powder pattern modelling. Acta Cryst 2002; A58: 190–200.

33.

Nakayama

Caton

Nova

. Assessment of the Alamar-Blue assay for cellular growth and viability in vitro. J Immunol Methods 1997; 204: 205–208.

34.

Cihlář

Buchal

Trunec

. Kinetics of thermal decomposition of hydroxyapatite bioceramics. J Mater Sci 1999; 34(24): 6121–6131.

35.

Landi

Tampieri

Celotti

. Densification behaviour and mechanisms of synthetic hydroxyapatites. J Eur Ceram Soc 2000; 20: 2377–2387.

36.

Nakamoto

. Infrared spectra of inorganic and coordination compounds, New York, NY: Wiley-Interscience, 1986.

37.

Driessens

FCM

Boltong

Maeyer

EAP

. The Ca/P range of nanoapatitic calcium phosphate cements. Biomaterials 2002; 23: 4011–4011.

38.

Ciesla

Schuth

. Ordered mesoporous materials. Micropor Mesopor Mater 1999; 27: 131–149.

39.

Yang

Bei

Wang

. Enhanced cell affinity of poly(D, L-lactide) by combining plasma treatment with collagen anchorage. Biomaterials 2002; 23: 2607–2607.

40.

Bos

Henny

Mei

. Physico-chemistry of initial microbial adhesive interactions – its mechanism and methods for study. FEMS Microbiol 1999; 23: 179–230.