In vivo study of a biodegradable orthopedic screw (MgYREZr-alloy) in a rabbit model for up to 12 months

Abstract

Biodegradable magnesium-based implants are currently being developed for use in orthopedic applications. The aim of this study was to investigate the acute, subacute, and chronic local effects on bone tissue as well as the systemic reactions to a magnesium-based (MgYREZr-alloy) screw containing rare earth elements. The upper part of the screw was implanted into the marrow cavity of the left femora of 15 adult rabbits (New Zealand White), and animals were euthanized 1 week, 12 weeks, and 52 weeks postoperatively. Blood samples were analyzed at set times, and radiographic examinations were performed to evaluate gas formation. There were no significant increased changes in blood values compared to normal levels. Histological examination revealed moderate bone formation with direct implant contact without a fibrous capsule. Histopathological evaluation of lung, liver, intestine, kidneys, pancreas, and spleen tissue samples showed no abnormalities. In summary, our data indicate that these magnesium-based screws containing rare earth elements have good biocompatibility and osteoconductivity without acute, subacute, or chronic toxicity.

Keywords

In vivo biodegradation magnesium alloy orthopedic screw

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References

Hofmann

. Biodegradable implants in traumatology: a review on the state-of-the-art. Arch Orthop Trauma Surg 1995; 114: 123–132.

Waizy H, Seitz J-M, Reifenrath J, et al. Biodegradable magnesium implants for orthopedic applications. J Mater Sci 2012. DOI: 10.1007/s10853-012-6572-2.

Witte

Hort

Vogt

. Degradable biomaterials based on magnesium corrosion. Curr Opin Solid St Mater Sci 2008; 12: 63–72.

Staiger

Pietak

Huadmai

. Magnesium and its alloys as orthopedic biomaterials: a review. Biomaterials 2006; 27: 1728–1734.

Witte

Kaese

Haferkamp

. In vivo corrosion of four magnesium alloys and the associated bone response. Biomaterials 2005; 26: 3557–3563.

Zreiqat

Howlett

Zannettino

. Mechanisms of magnesium-stimulated adhesion of osteoblastic cells to commonly used orthopaedic implants. J Biomed Mater Res 2002; 62: 175–184.

Castellani

Lindtner

Hausbrandt

. Bone-implant interface strength and osseointegration: biodegradable magnesium alloy versus standard titanium control. Acta Biomater 2011; 7: 432–440.

Reifenrath

Krause

Bormann

. Profound differences in the in-vivo-degradation and biocompatibility of two very similar rare-earth containing Mg-alloys in a rabbit model. Matwiss u Werkstofftech 2010; 41: 1054–1061.

Thomann

Krause

Bormann

. Comparision of the resorbable magnesium alloys LAE442 and MgCa0.8 concerning their mechanical properties, their progress of degradation and the bone-implant-contact after 12 months implantation duration in a rabbit model. Matwiss u Werkstofftech 2009; 40: 82–87.

10.

Haley

. Pharmacology and toxicology of the rare earth elements. J Pharm Sci 1965; 54: 663–670.

11.

Hirano

Suzuki

. Exposure, metabolism, toxicity of rare earths and related compounds. Environ Health Perspect 1996; 104(Suppl 1): 85–95.

12.

Yumiko

Yukari

Yasuhide

. Differences in behavior among the chlorides of seven rare earth elements administered intravenously to rats. Fundam Appl Toxicol 1997; 37: 106–116.

13.

Drynda

Deinet

Braun

. Rare earth metals used in biodegradable magnesium-based stents do not interfere with proliferation of smooth muscle cells but do induce the upregulation of inflammatory genes. J Biomed Mater Res A 2009; 91: 360–369.

14.

Feyerabend

Fischer

Holtz

. Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines. Acta Biomater 2010; 6: 1834–1842.

15.

Donath

Breuner

. A method for the study of undecalcified bones and teeth with attached soft tissue. The Säge-Schliff (sawing and grinding) technique. J Oral Pathol 1982; 11: 318–326.

16.

An YH and Martin KL. Handbook of histology methods for bone and cartilage. New York: Humana Press.

17.

Aghion

Levy

Ovadia

. In vivo behavior of biodegradable Mg-Nd-Y-Zr-Ca alloy. J Mater Sci Mater Med 2012; 23: 805–812.

18.

Zhang

. In vivo corrosion behavior of Mg-Mn-Zn alloy for bone implant application. J Biomed Mater Res A 2007; 83: 703–711.

19.

Zhang

. In vivo evaluation of biodegradable magnesium alloy bone implant in the first 6 months implantation. J Biomed Mater Res A 2009; 90: 882–893.

20.

Witte

. The history of biodegradable magnesium implants: a review. Acta Biomater 2010; 6: 1680–1692.

21.

Lou

. The development of binary Mg-Ca alloys for use as biodegradable materials within bone. Biomaterials 2008; 29: 1329–1344.

22.

Hanzi

Gerber

Schinhammer

. On the in vitro and in vivo degradation performance and biological response of new biodegradable Mg-Y-Zn alloys. Acta Biomater 2010; 6: 1824–1833.

23.

Zhang

Zhao

. Research on an Mg-Zn alloy as a degradable biomaterial. Acta Biomater 2010; 6: 626–640.

24.

Huehnerschulte

Angrisani

Rittershaus

. In vivo corrosion of two novel magnesium alloys ZEK100 and AX30 and their mechanical suitability as biodegradable implants. Materials 2011; 4: 1144–1167.

25.

Ullmann

Reifenrath

Dziuba

. In vivo degradation behavior of the magnesium alloy LANd442 in rabbit tibiae. Materials 2011; 4: 2197–2218.

26.

Witte

Ulrich

Palm

. Biodegradable magnesium scaffolds: Part II: peri-implant bone remodeling. J Biomed Mater Res A 2007; 81: 757–765.

27.

Reifenrath

Bormann

Meyer-Lindenberg

Magnesium alloys as promising degradable implant materials in orthopaedic research. In: Czerwinski

(eds). Magnesium alloys - Corrosion and surface treatments, Rijek: Intech, 2011, pp. 93–108.