Recently, silk fibroin scaffolds have been introduced as novel and promising biomaterials in the field of cardiac tissue engineering. This study was designed to compare infiltration, proliferation, and cardiac differentiation potential of menstrual blood-derived stem cells (MenSCs) versus bone marrow-derived mesenchymal stem cells (BMSCs) in Bombyx mori-derived silk scaffold. Our primary data revealed that the fabricated scaffold has mechanical and physical qualities suitable for cardiac tissue engineering. The MenSCs tracking in scaffolds using immunofluorescent staining and scanning electron microscopy confirmed MenSCs attachment, penetration, and distribution within the porous scaffold matrix. Based on proliferation assay using propidium iodide DNA quantification, the significantly higher level of growth rates of both MenSCs and BMSCs was documented in scaffolds than that in two-dimensional culture (p < 0.01). The expression level of TNNT2, a bona fide cardiac differentiation marker, in BMSCs differentiated on silk scaffolds was markedly higher than those cultured in two-dimensional culture indicating the improvement of cardiac differentiation in the silk scaffolds. Furthermore, differentiated MenSCs exhibited higher expression of TNNT2 compared with induced BMSCs. It seems that silk scaffold-seeded MenSCs could be viewed as a novel, safe, natural, and accessible construct for cardiac tissue engineering.
SilvestriABoffitoMSartoriS. Biomimetic materials and scaffolds for myocardial tissue regeneration. Macromol Biosci2013; 13: 984–1019.
3.
PiaoHKwonJSPiaoS. Effects of cardiac patches engineered with bone marrow-derived mononuclear cells and PGCL scaffolds in a rat myocardial infarction model. Biomaterials2007; 28: 641–649.
OmenettoFGKaplanDL. New opportunities for an ancient material. Science2010; 329: 528–531.
6.
VepariCKaplanDL. Silk as a biomaterial. Prog Polym Sci2007; 32: 991–1007.
7.
HardyJGR€omerLMScheibelTR. Polymeric materials based on silk proteins. Polymer2008; 49: 4309–4327.
8.
BhardwajNNguyenQTChenAC. Potential of 3-D tissue constructs engineered from bovine chondrocytes/silk fibroin-chitosan for in vitro cartilage tissue engineering. Biomaterials2011; 32: 5773–5781.
9.
TalukdarSMandalMHutmacherDW. Engineered silk fibroin protein 3D matrices for in vitro tumor model. Biomaterials2011; 32: 2149–2159.
10.
PanilaitisBAltmanGHChenJ. Macrophage responses to silk. Biomaterials2003; 24: 3079–3085.
11.
ChangGKimHJKaplanD. Porous silk scaffolds can be used for tissue engineering annulus fibrosus. Eur Spine J2007; 16: 1848–1857.
12.
MobiniSHoyerBSolati-HashjinM. Fabrication and characterization of regenerated silk scaffolds reinforced with natural silk fibers for bone tissue engineering. J Biomed Mater Res A2013; 101: 2392–2404.
13.
PatraCTalukdarSNovoyatlevaT. Silk protein fibroin from Antheraea mylitta for cardiac tissue engineering. Biomaterials2012; 33: 2673–2680.
14.
YangMCWangSSChouNK. The cardiomyogenic differentiation of rat mesenchymal stem cells on silk fibroin-polysaccharide cardiac patches in vitro. Biomaterials2009; 30: 3757–3765.
15.
HaghaniKBakhtiyariSNouriAM. In vitro study of the differentiation of bone marrow stromal cells into cardiomyocyte-like cells. Mol Cell Biochem2012; 361: 315–320.
16.
TuanRSBolandGTuliR. Adult mesenchymal stem cells and cell-based tissue engineering. Arthritis Res Ther2003; 5: 32–45.
MusinaRABelyavskiAVTarusovaOV. Endometrial mesenchymal stem cells isolated from the menstrual blood. Bull Exp Biol Med2008; 145: 539–543.
19.
KhanmohammadiMKhanjaniSBakhtyariMS. Proliferation and chondrogenic differentiation potential of menstrual blood- and bone marrow-derived stem cells in two-dimensional culture. Int J Hematol2012; 95: 484–493.
20.
DarziSZarnaniAHJeddi-TehraniM. Osteogenic differentiation of stem cells derived from menstrual blood versus bone marrow in the presence of human platelet releasate. Tissue Eng Part A2012; 18: 1720–1728.
21.
KazemnejadSAkhondiMMSoleimaniM. Characterization and chondrogenic differentiation of menstrual blood-derived stem cells on a nanofibrous scaffold. Int J Artif Organs2012; 35: 55–66.
22.
Khanjani S, Khanmohammadi M, Talebi S, et al. Efficient differentiation of menstrual blood derived stem cells in to functional hepatocyte-like cells using various conversion protocols. J Tissue Eng Regen Med 2013 (in press).
23.
HidaNNishiyamaNMiyoshiS. Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal cells. Stem Cells2008; 26: 1695–1704.
24.
MartinBM. Tissue culture techniques, Boston: Springer, 1994, pp. 247–247.
25.
ZhouLLiuYLuL. Cardiac gene activation analysis in mammalian non-myoblasic cells by Nkx2-5, Tbx5, Gata4 and Myocd. PLoS One2012; 7: e48028–e48028.
26.
NaguehSFShahGWuY. Altered titin expression, myocardial stiffness, and left ventricular function in patients with dilated cardiomyopathy. Circulation2004; 110: 155–162.
27.
WatanabeSShiteJTakaokaH. Myocardial stiffness is an important determinant of the plasma brain natriuretic peptide concentration in patients with both diastolic and systolic heart failure. Eur Heart J2006; 27: 832–838.
28.
RoederBAKokiniKSturgisJE. Tensile mechanical properties of three-dimensional type I collagen extracellular matrices with varied microstructure. J Biomech Eng2002; 124: 214–222.
29.
MisofKLandisWJKlaushoferK. Collagen from the osteogenesis imperfecta mouse model (oim) shows reduced resistance against tensile stress. J Clin Invest1997; 100: 40–45.
30.
GaoJCrapoPMWangY. Macroporous elastomeric scaffolds with extensive micropores for soft tissue engineering. Tissue Eng2006; 12: 917–925.
31.
WangYAmeerGASheppardBJ. A tough biodegradable elastomer. Nat Biotechnol2002; 20: 602–606.
32.
BoublikJParkHRadisicM. Mechanical properties and remodeling of hybrid cardiac constructs made from heart cells, fibrin, and biodegradable, elastomeric knitted fabric. Tissue Eng2005; 11: 1122–1132.
PokSMyersJDMadihallySV. A multilayered scaffold of a chitosan and gelatin hydrogel supported by a PCL core for cardiac tissue engineering. Acta Biomater2013; 9: 5630–5642.
35.
GarlottaDA. Literature review of poly (lactic acid). J Polym Environ2001; 9: 63–84.
ChoiYSMatsudaKDustingGJ. Engineering cardiac tissue in vivo from human adipose-derived stem cells. Biomaterials2010; 31: 2236–2242.
38.
SingelynJMDeQuachJASeif-NaraghiSB. Naturally derived myocardial matrix as an injectable scaffold for cardiac tissue engineering. Biomaterials2009; 30: 5409–5416.
39.
KaramJPMuscariCMontero-MeneiCN. Combining adult stem cells and polymeric devices for tissue engineering in infarcted myocardium. Biomaterials2012; 33: 5683–5695.
40.
Venugopal JR, Prabhakaran MP, Mukherjee S, et al. Biomaterial strategies for alleviation of myocardial infarction. J R Soc Interface 2012; 66: 1–19.
41.
Chan BP and Leong KW. Scaffolding in tissue engineering: general approaches and tissue specific considerations. Eur Spine J 2008; 4: 467–479.
42.
RadisicMYangLBoublikJ. Medium perfusion enables engineering of compact and contractile cardiac tissue. Am J Physiol Heart Circ Physiol2004; 286: H507–H516.
ZhangMJLiuBXiaW. Could cells from menstrual blood be a new source for cell-based therapies?Med Hypotheses2009; 72: 252–254.
45.
MandalBBKunduSC. Cell proliferation and migration in silk fibroin 3D scaffolds. Biomaterials2009; 30: 2956–2965.
46.
ZhangYFanWMaZ. The effects of pore architecture in silk fibroin scaffolds on the growth and differentiation of mesenchymal stem cells expressing BMP7. Acta Biomater2010; 6: 3021–3028.
47.
MurphyCMHaughMGO'BrienFJ. The effect of mean pore size on cell attachment, proliferation and migration in collagen-glycosaminoglycan scaffolds for bone tissue engineering. Biomaterials2010; 31: 461–466.
48.
YangMCChiNHChouNK. The influence of rat mesenchymal stem cell CD44 surface markers on cell growth, fibronectin expression, and cardiomyogenic differentiation on silk fibroin – hyaluronic acid cardiac patches. Biomaterials2010; 31: 854–862.
49.
MogensenJMurphyRTShawT. Severe disease expression of cardiac troponin C and T mutations in patients with idiopathic dilated cardiomyopathy. J Am Coll Cardiol2004; 44: 2033–2040.
