To develop biocompatible composite microspheres for novel hemostatic use, we designed and prepared a novel biomaterial, composite microspheres consisting of carboxymethyl chitosan, sodium alginate, and collagen (CSCM). The ultra-structure of CSCM was investigated by scanning electron microscopy assay. In hemostatic function experiment, it was found that CSCM could facilitate platelet adherence, platelet aggregation, and platelet activation in vitro. Besides, the maximum swelling of CSCM submerged in PBS for 50 min was over 300% of that exhibited by commercial hemostatic compound microporous polysaccharide haemostatic powder (CMPHP). In addition, CSCM exhibited good biodegradability and non-cytotoxicity. These results demonstrated that CSCM may be useful in platelet plug formation, and this study would provide important information for further research on hemostasis experiment in vivo.
DevlinJJKircherSKozenBG. Comparison of ChitoFlex(R), CELOX, and QuikClot(R) in control of hemorrhage. J Emerg Med2011; 41: 237–245.
2.
LittlejohnLFDevlinJJKircherSS. Comparison of Celox-A, ChitoFlex, WoundStat, and Combat Gauze hemostatic agents versus standard gauze dressing in control of hemorrhage in a swine model of penetrating trauma. Acad Emerg Med2011; 18: 340–350.
3.
RongJJLiangMXuanFQ. Alginate-calcium microsphere loaded with thrombin: a new composite biomaterial for hemostatic embolization. Int J Biol Macromol2015; 75: 479–488.
4.
LinCYLinSJYangYC. Biodegradable polymeric microsphere-based vaccines and their applications in infectious diseases. Hum Vaccines Immunother2015; 11: 650–656.
5.
SayedEGHusseinAKKhaledKA. Improved corneal bioavailability of ofloxacin: biodegradable microsphere-loaded ion-activated in situ gel delivery system. Drug Des Dev Ther2015; 9: 1427–1435.
6.
VournakisJNDemchevaMWhitsonAB. The RDH bandage: hemostasis and survival in a lethal aortotomy hemorrhage model. J Surg Res2003; 113: 1–5.
7.
KheirabadiBSAchesonEMDeguzmanR. The potential utility of fibrin sealant dressing in repair of vascular injury in swine. J Trauma2007; 62: 94–103.
8.
BaikSHKimJHChoHH. Development and analysis of a collagen-based hemostatic adhesive. J Surg Res2010; 164: E221–E228.
9.
KunioNRRihaGMWatsonKM. Chitosan based advanced hemostatic dressing is associated with decreased blood loss in a swine uncontrolled hemorrhage model. Am J Surg2013; 205: 505–510.
10.
HuangXFJiaJWWangZK. A novel chitosan-based sponge coated with self-assembled thrombin/tannic acid multilayer films as a hemostatic dressing. Chin J Polym Sci2015; 33: 284–290.
11.
MisgavMKenetGMartinowitzU. Chitosan-based dressing for the treatment of external/accessible bleedings in children with bleeding tendency. J Pediatr Hematol Oncol2014; 36: 140–142.
12.
HeQGongKAoQ. Positive charge of chitosan retards blood coagulation on chitosan films. J Biomater Appl2013; 27: 1032–1045.
13.
TaskinAKYasarMOzaydinI. The hemostatic effect of calcium alginate in experimental splenic injury model. Ulusal travma ve acil cerrahi dergisi (Turk J Trauma Emerg Surg)2013; 19: 195–199.
14.
PinkasOZilbermanM. Effect of hemostatic agents on properties of gelatin-alginate soft tissue adhesives. J Biomater Sci Polym Ed2014; 25: 555–573.
15.
GardnerRL. Application of alginate gels to the study of mammalian development. Methods Mol Biol2004; 254: 383–392.
BloomfieldMRKlikaAKMolloyRM. Prospective randomized evaluation of a collagen/thrombin and autologous platelet hemostatic agent during total knee arthroplasty. J Arthroplasty2012; 27: 695–702.
18.
ErdoganDvan GulikTM. Evolution of fibrinogen-coated collagen patch for use as a topical hemostatic agent. J Biomed Mater Res Part B2008; 85: 272–278.
19.
FujimotoYKobayashiTKomoriM. Modified hemostatic technique using microfibrillar collagen hemostat in endoscopic endonasal transsphenoidal surgery: technical note. Neurologia Medico-Chirurgica2014; 54: 617–621.
20.
LewisKMSpaziererDSlezakP. Swelling, sealing, and hemostatic ability of a novel biomaterial: a polyethylene glycol-coated collagen pad. J Biomater Appl2014; 29: 780–788.
21.
LiapinaLAUl'ianovAMPastorovaVE. Effect of chronic intramuscular administration of low molecular weight heparin-collagen complex on hemostatic indices and development of alloxan-induced diabetes. Izvestiia Akademii Nauk Seriia Biologicheskaia/Rossiiskaia Akademiia Nauk2006; 33(3): 381–384. .
22.
HuangXSunYNieJ. Using absorbable chitosan hemostatic sponges as a promising surgical dressing. Int J Biol Macromol2015; 75: 322–329.
23.
PusateriAEDelgadoAVDickEJ Jr. Application of a granular mineral-based hemostatic agent (QuikClot) to reduce blood loss after grade V liver injury in swine. J Trauma2004; 57: 555–562.
24.
AlamHBChenZJaskilleA. Application of a zeolite hemostatic agent achieves 100% survival in a lethal model of complex groin injury in Swine. J Trauma2004; 56: 974–983.
25.
Zohuriaan-MehrMJOmidianHDoroudianiS. Advances in non-hygienic applications of superabsorbent hydrogel materials. J Mater Sci2010; 45: 5711–5735.
26.
WagnerWRPachenceJMRistichJ. Comparative in vitro analysis of topical hemostatic agents. J Surg Res1996; 66: 100–108.
27.
MorimotoNMaharaAShimaK. The rapid inactivation of porcine skin by applying high hydrostatic pressure without damaging the extracellular matrix. BioMed Res Int2015; 2015: 587247.
28.
BonifaziMAloisiAMCeccarelliI. Platelet adhesion and aggregation on polyethylene: effect of exhaustive exercise. J Biomed Mater Res Part B2004; 68B: 53–58.
29.
IzuhaHHattoriMIgariT. Changes in platelet aggregation during cardiopulmonary bypass: comparison of poly-2-methoxyethylacrylate and heparin as a circuit coating material. J Artif Organs2005; 8: 41–46.
30.
VasconcellosFCGoulartGASBeppuMM. Production and characterization of chitosan microparticles containing papain for controlled release applications. Powder Technol2011; 205: 65–70.
