Effect of GO-Fe 3 O 4 and rotating magnetic field on cellular metabolic activity of mammalian cells

Abstract

The effect of hybrid material—graphene flakes with Fe₃O₄ nanospheres (GO-Fe₃O₄), graphene oxide (GO) and magnetite nanospheres (Fe₃O₄) in rotating magnetic field on mammalian cells metabolism has been studied. Several reports shown that exposure to magnetic field may have influence on cellular membrane permeability. Thus, the aim of presented study was to determine the cellular response of L929 fibroblast cells to nanomaterials and rotating magnetic field for 8-h exposure experiment. The GO had tendency to adsorb proteins, thus cell metabolism was decreased and the effect of that mechanism was enhanced by impact of nanospheres and rotating magnetic field. The highest reduction of cellular metabolism was recorded for WST-1 and NR assays at concentration 100 µg/mL of all tested nanomaterials and magnetic induction value 10.06 mT. The lactate dehydrogenase leakage assay has shown significant changes in membrane permeability. Further studies need to be carried out to precisely determine the mechanism of that process.

Keywords

Magnetite nanospheres graphene oxide hyperthermia rotating magnetic field magnetoporation

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