Microfluidic system for synthesis of nanofibrous conductive hydrogel and muscle differentiation

Microscale hydrogels can be synthesized within microfluidic systems and subsequently assembled to make tissues composed of units such as myofibers in muscle tissue. Importantly, the nanofibrous surface of hydrogels is essential for tissue engineering aims due to inducing beneficial cell–surface interactions. In this study, a new microfluidic platform, embedded with a hydrogel, was introduced that allowed for performing multiple non-parallel steps for the synthetic approaches. Satellite cells, isolated from skeletal tissues of 10-day Naval Medical Research Institute-murine were cultured on the prepared hydrogel within the microfluidic system. The normal proliferation of satellite cells occurred after the employment of continuous perfusion cell culture. Interestingly, the positive results of the immuno-staining assay along with the cellular bridge formation between hydrogel fragments confirmed the muscle differentiation of seeded satellite cells. Further on, COMSOl simulations anticipated that the thermodynamic conditions of the microfluidic system during hydrogel synthesis had to be kept steady while a shear stress value of 15 × 10⁻⁶ Pa was calculated, exhibiting a cell culture condition free of environmental stress.