Layered hydroxide/polydopamine/hyaluronic acid functionalized magnesium alloys for enhanced anticorrosion,biocompatibility and antithrombogenicity in vascular stents
Restricted accessResearch articleFirst published online March, 2020
Layered hydroxide/polydopamine/hyaluronic acid functionalized magnesium alloys for enhanced anticorrosion,biocompatibility and antithrombogenicity in vascular stents
Magnesium alloys are promising cardiovascular stent materials due to the favourable physical properties and complete biodegradability in vivo. However, the rapid degradation, poor cytocompatibility and tendency of thrombogenesis hinder practical clinical applications. In order to solve these problems, a facile and highly efficient strategy of alkali treatment combined with subsequent layer-by-layer assembly was used to fabricate a multifunctional coating. A bottom layer hydroxyl (–OH) with negative charge after alkali treatment first formed a solid bond with magnesium matrix to provide a rough outer surface for the further immobilization of functional biomolecules. Afterwards, polydopamine and hyaluronic acid were successively immobilized on alkali-treated magnesium surface via strong electrostatic adsorption and covalent bonding between carboxyl group of hyaluronic acid and amine or hydroxyl of polydopamine to form magnesium/OH/polydopamine/hyaluronic acid. Hydroxyl significantly improves the corrosion resistance while polydopamine and hyaluronic acid layers act as a further barrier to provide better anticorrosion. A balance between biocompatibility and antithrombogenicity has been achieved by adjusting the content of hyaluronic acid on polydopamine surface. The multifunctional magnesium/OH/polydopamine/hyaluronic acid coating with lower hyaluronic acid concentrations expose more active sites of polydopamine molecules to promote endothelial cell proliferation while retaining the intrinsic antithrombogenic function of hyaluronic acid to offer a potential application for vascular stents.
AgarwalSCurtinJDuffyB, et al.
Biodegradable magnesium alloys for orthopaedic applications: a review on corrosion, biocompatibility and surface modifications. Mater Sci Eng C Mater Biol Appl2016;
68: 948–963.
2.
GhimireGSpiroJKharbandaR, et al.
Initial evidence for the return of coronary vasoreactivity following the absorption of bioabsorbable magnesium alloy coronary stents. Eurointerv J Eurointerv2009;
4: 481.
3.
ZartnerPCesnjevarRSingerH, et al.
First successful implantation of a biodegradable metal stent into the left pulmonary artery of a preterm baby. Catheter Cardiovasc Interv2005;
66: 590–594.
4.
ZengRDietzelWWitteF, et al.
Progress and challenge for magnesium alloys as biomaterials. Adv Eng Mater2008;
10: B3–B14.
5.
ThormannUAltVHeimannL, et al.
The biocompatibility of degradable magnesium interference screws: an experimental study with sheep. Biomed Res Int2015;
Article ID: 943603.
6.
BobbyKMRamanRKS.In vitro degradation and mechanical integrity of calcium-containing magnesium alloys in modified-simulated body fluid. Biomaterials2008;
29: 2306–2314.
7.
LiuJXiT.Enhanced anti-corrosion ability and biocompatibility of PLGA coatings on MgZnYNd alloy by BTSE-APTES pre-treatment for cardiovascular stent. J Mater Sci Technol2016;
32: 845–857.
8.
WangPXiongPLiuJ, et al.
A silk-based coating containing GREDVY peptide and heparin on Mg–Zn–Y–Nd alloy: improved corrosion resistance, hemocompatibility and endothelialization. J Mater Chem B2018;
6: 966–978.
9.
ZhengJZhaoNWatsonN, et al.
In vitro biocompatibility and endothelialization of novel magnesium-rare earth alloys for improved stent applications. PLoS ONE2014;
9: e98674.
10.
ZhangXZhangGZhangH, et al.
Surface immobilization of heparin and chitosan on titanium to improve hemocompatibility and antibacterial activities. Colloids Surf B Biointerf2018;
172: 338–345.
11.
ZhangL-CXuMHuY-D, et al.
Biofunctionization of biodegradable magnesium alloy to improve the in vitro corrosion resistance and biocompatibility. Appl Surf Sci2018;
451: 20–31.
12.
LeeHLeeBPMessersmithPB.A reversible wet/dry adhesive inspired by mussels and geckos. Nature2007;
448: 338–341.
13.
YangYQiPDingY, et al.
A biocompatible and functional adhesive amine-rich coating based on dopamine polymerization. J Mater Chem B2015;
3: 72–81.
14.
WuCHanPLiuX, et al.
Mussel-inspired bioceramics with self-assembled Ca-P/polydopamine composite nanolayer: preparation, formation mechanism, improved cellular bioactivity and osteogenic differentiation of bone marrow stromal cells. Acta Biomater2014;
10: 428–438.
15.
ChengCLiSNieS, et al.
General and biomimetic approach to biopolymer-functionalized graphene oxide nanosheet through adhesive dopamine. Biomacromolecules2012;
13: 4236–4246.
16.
YangZTuQZhuY, et al.
Mussel-inspired coating of polydopamine directs endothelial and smooth muscle cell fate for re-endothelialization of vascular devices. Adv Healthc Mater2012;
1: 548–559.
17.
XuePLiQLiY, et al.
Surface modification of poly(dimethylsiloxane) with polydopamine and hyaluronic acid to enhance hemocompatibility for potential applications in medical implants or devices. ACS Appl Mater Interf2017;
9: 33632–33644.
18.
MaLQinHChengC, et al.
Mussel-inspired self-coating at macro-interface with improved biocompatibility and bioactivity via dopamine grafted heparin-like polymers and heparin. J Mater Chem B2014;
2: 363–375.
19.
KangHSongXWangZ, et al.
High-performance and fully renewable soy protein isolate-based film from microcrystalline cellulose via bio-inspired poly(dopamine) surface modification. ACS Sustain Chem Eng2016;
4: 4354–4360.
20.
LiJ-WZhangG-LGongZ-T.Nd isotope evidence for dust accretion to a soil chronosequence in Hainan Island. Catena2013;
101: 24–30.
21.
LaiJYMaDHChengHY, et al.
Ocular biocompatibility of carbodiimide cross-linked hyaluronic acid hydrogels for cell sheet delivery carriers. J Biomater Sci Polym Ed2010;
21: 359–376.
22.
ChoYSeoSYParkBJ, et al.
Silicone rubber with mussel-inspired adhesive coatings for enhancing antifouling property and blood compatibility. Macromol Res2017;
25: 841–848.
23.
IioKFurukawaKITsudaE, et al.
Hyaluronic acid induces the release of growth factors from platelet-rich plasma. Asia Pac J Sports Med Arthrosc Rehabil Technol2016;
4: 27–32.
24.
Le ThiPLeeYKwonHJ, et al.
Tyrosinase-mediated surface coimmobilization of heparin and silver nanoparticles for antithrombotic and antimicrobial activities. ACS Appl Mater Interf2017;
9: 20376–20384.
25.
HeXZhangGWangX, et al.
Biocompatibility, corrosion resistance and antibacterial activity of TiO2/CuO coating on titanium. Ceram Int2017;
43: 16185–16195.
26.
ChuahYJKohYTLimK, et al.
Simple surface engineering of polydimethylsiloxane with polydopamine for stabilized mesenchymal stem cell adhesion and multipotency. Sci Rep2015;
5: 18162.
27.
CamargoWATakemotoSHoekstraJW, et al.
Effect of surface alkali-based treatment of titanium implants on ability to promote in vitro mineralization and in vivo bone formation. Acta Biomater2017;
57: 511–523.
28.
DuXLiLLiJ, et al.
UV-triggered dopamine polymerization: control of polymerization, surface coating, and photopatterning. Adv Mater Weinheim2014;
26: 8029–8033.
29.
YangYLuYLuM, et al.
Functional nanocomposites prepared by self-assembly and polymerization of diacetylene surfactants and silicic acid. J Am Chem Soc2003;
125: 1269–1277.
30.
NetoAICibraoACCorreiaCR, et al.
Nanostructured polymeric coatings based on chitosan and dopamine-modified hyaluronic acid for biomedical applications. Small2014;
10: 2459–2469.
31.
OssCJVJuLChaudhuryMK, et al.
Estimation of the polar parameters of the surface tension of liquids by contact angle measurements on gels. J Colloid Interf Sci1989;
128: 313–319.
32.
HuangRLiuXYeH, et al.
Conjugation of hyaluronic acid onto surfaces via the interfacial polymerization of dopamine to prevent protein adsorption. Langmuir2015;
31: 12061–12070.
33.
GaoHZhangMZhaoJ, et al.
In vitro and in vivo degradation and mechanical properties of ZEK100 magnesium alloy coated with alginate, chitosan and mechano-growth factor. Mater Sci Eng C Mater Biol Appl2016;
63: 450–461.
34.
ChayaAYoshizawaSVerdelisK, et al.
In vivo study of magnesium plate and screw degradation and bone fracture healing. Acta Biomater2015;
18: 262–269.
35.
LiJHeXZhangG, et al.
Electrochemical corrosion, wear and cell behavior of ZrO2/TiO2 alloyed layer on Ti-6Al-4V. Bioelectrochemistry2018;
121: 105–114.
36.
ChenSChenYLeiY, et al.
Novel strategy in enhancing stability and corrosion resistance for hydrophobic functional films on copper surfaces. Electrochem Commun2009;
11: 1675–1679.
37.
YuFChenSChenY, et al.
Experimental and theoretical analysis of polymerization reaction process on the polydopamine membranes and its corrosion protection properties for 304 stainless steel. J Mol Struct2010;
982: 152–161.
38.
LiHPengFWangD, et al.
Layered double hydroxide/poly-dopamine composite coating with surface heparinization on Mg alloys: improved anticorrosion, endothelialization and hemocompatibility. Biomater Sci2018;
6: 1846–1858.
39.
ZhouXOuYangJLiL, et al.
In vitro and in vivo anti-corrosion properties and bio-compatibility of 5β-TCP/Mg-3Zn scaffold coated with dopamine-gelatin composite. Surf Coat Technol2019;
374: 152–163.
40.
YuKChenLZhaoJ, et al.
In vivo biocompatibility and biodegradation of a Mg-15%Ca3(PO4)2 composite as an implant material. Mater Lett2013;
98: 22–25.
41.
MavrogonatouEKletsasD.High osmolality activates the G1 and G2 cell cycle checkpoints and affects the DNA integrity of nucleus pulposus intervertebral disc cells triggering an enhanced DNA repair response. DNA Repair (Amst)2009;
8: 930–943.
42.
CutaiaMBlackADCohenI, et al.
Alkaline stress-induced apoptosis in human pulmonary artery endothelial cells. Apoptosis2005;
10: 1457–1467.
43.
RyuJKuSHLeeH, et al.
Mussel-inspired polydopamine coating as a universal route to hydroxyapatite crystallization. Adv Funct Mater2010;
20: 2132–2139.
44.
FuJChuahYJAngWT, et al.
Optimization of a polydopamine (PD)-based coating method and polydimethylsiloxane (PDMS) substrates for improved mouse embryonic stem cell (ESC) pluripotency maintenance and cardiac differentiation. Biomater Sci2017;
5: 1156–1173.
45.
MengHLiYFaustM, et al.
Hydrogen peroxide generation and biocompatibility of hydrogel-bound mussel adhesive moiety. Acta Biomater2015;
17: 160–169.
46.
JoungYKYouSSParkKM, et al.
In situ forming, metal-adhesive heparin hydrogel surfaces for blood-compatible coating. Colloids Surf B Biointerf2012;
99: 102–107.
47.
LiJZhangKYangP, et al.
Human vascular endothelial cell morphology and functional cytokine secretion influenced by different size of HA micro-pattern on titanium substrate. Colloids Surf B Biointerf2013;
110: 199–207.
48.
ZhangHXieLShenX, et al.
Catechol/polyethyleneimine conversion coating with enhanced corrosion protection of magnesium alloys: potential applications for vascular implants. J Mater Chem B2018;
6: 6936–6949.
49.
CherngW-JPanY-HWuT-C, et al.
Hemocompatibility and adhesion of heparin/dopamine and heparin/collagen self-assembly multilayers coated on a titanium substrate. Appl Surf Sci2018;
463: 732–740.
