Platelet-derived growth factor AA-modified electrospun fibers promote tendon healing

Abstract

Platelet-derived growth factor AA (PDGF-AA) is an important promoter of tissue injury repair and might be a candidate for improving the mechanical properties of repaired tendons. Here, we designed a PDGF-AA-modified poly(lactide-co-glycolide) acid (PLGA) electrospun fibers to promote tendon rehabilitation after injury. In the present study, we grafted PDGF-AA on the surface of PLGA. In structural experiments, we found that the hydrophilicity of PLGA containing PDGF-AA (PLGA-PDGF-AA) increased, but the strength of the material did not change significantly. Moreover, no significant changes in tendon cell proliferation and viability were observed in the PLGA-PDGF-AA treatment compared with the control group. The mouse tendon injury model (n = 9) experiment illustrated that PLGA-PDGF-AA effectively promoted tendon healing, and we confirmed that PLGA-PDGF-AA promoted collagen synthesis and deposition by immunohistochemistry and RT-PCR. Moreover, the mechanical strength of PLGA-PDGF-AA-treated mouse (n = 9) tendon tissue was also higher than that of the PLGA-treated group alone. In conclusion, PLGA-PDGF-AA promoted regeneration after tendon injury and serves as a potential adjuvant material for surgical tendon injury repair.

Keywords

platelet-derived growth factor AA tendon injury repair electrospun fibers poly(lactide-co-glycolide) acid polymer

Get full access to this article

View all access options for this article.

References

Butler

Juncosa-Melvin

Boivin

, et al. Functional tissue engineering for tendon repair: a multidisciplinary strategy using mesenchymal stem cells, bioscaffolds, and mechanical stimulation. J Orthop Res 2008; 26(1): 1–9.

Sánchez

Anitua

, et al. Comparison of surgically repaired Achilles tendon tears using platelet-rich fibrin matrices. Am J Sports Med 2007; 35(2): 245–251.

Morita

Dakin

Snelling

SJB

, et al. Cytokines in tendon disease: a Systematic Review. Bone Jt Res 2017; 6(12): 656–664.

Wang

Tang

, et al. Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway. Cell Prolif 2019; 52(4): e12650.

Chai

Leong

. Biomaterials approach to expand and direct differentiation of stem cells. Mol Ther 2007; 15(3): 467–480.

Roman

Mangera

Osman

, et al.

Developing a tissue engineered repair material for treatment of stress urinary incontinence and pelvic organ prolapse—which cell source?

Neurourol Urodynamics 2014; 33(5): 531–537.

Evrova

Houska

Welti

, et al. Bioactive, elastic, and biodegradable emulsion electrospun degrapol tube delivering PDGF-BB for tendon rupture repair. Macromol Biosci 2016; 16(7): 1048–1063.

Evrova

Bürgisser

Ebnöther

, et al. Elastic and surgeon friendly electrospun tubes delivering PDGF-BB positively impact tendon rupture healing in a rabbit Achilles tendon model. Biomaterials 2020; 232(1): 119722.

Fujimaki

Uchida

Inoue

, et al. Polyglycolic acid-collagen tube combined with collagen-binding basic fibroblast growth factor accelerates gait recovery in a rat sciatic nerve critical-size defect model. J Biomed Mater Res B Appl Biomater 2020; 108(2): 326–332.

10.

Lim

Liau

, et al. Current progress in tendon and ligament tissue engineering. Tissue Eng Regen Med 2019; 16(6): 549–571.

11.

Fretto

Snape

Tomlinson

, et al. Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor. J Biol Chem 1993; 268(5): 3625–3631.

12.

Heldin

Westermark

. Mechanism of action and in vivo role of platelet-derived growth factor. Physiol Rev 1999; 79(4): 1283–1316.

13.

Harvey

Flamenco

Fan

. A Tppp3(+)Pdgfra(+) tendon stem cell population contributes to regeneration and reveals a shared role for PDGF signalling in regeneration and fibrosis. Nat Cel Biol 2019; 21(12): 1490–1503.

14.

Lawton

Price

Runge

, et al. Air plasma treatment of submicron thick PDMS polymer films: effect of oxidation time and storage conditions. Colloids Surf A Physicochem Eng Aspects 2005; 253(1): 213–215.

15.

Peng

, et al. Effect of mechanical stimulation combined with platelet-rich plasma on healing of the rotator cuff in a murine model. Am J Sports Med 2022; 50(5): 1358–1368.

16.

Mills

Kelly

Logan

, et al. Succinate dehydrogenase supports metabolic repurposing of mitochondria to drive inflammatory macrophages. Cell 2016; 167(2): 457–470.

17.

Meisel

Hinterleitner

Pacis

, et al. Microbial signals drive pre-leukaemic myeloproliferation in a Tet2-deficient host. Nature 2018; 557(7706): 580–584.

18.

Mehrasa

Asadollahi

Ghaedi

, et al. Electrospun aligned PLGA and PLGA/gelatin nanofibers embedded with silica nanoparticles for tissue engineering. Int J Biol Macromol 2015; 79(1): 687–695.

19.

Russo

El Khatib

di Marcantonio

, et al. Tendon biomimetic electrospun PLGA fleeces induce an early epithelial-mesenchymal transition and tenogenic differentiation on amniotic epithelial stem cells. Cells 2020; 9(2): 303.

20.

El Khatib

Mauro

Di Mattia

, et al. Electrospun PLGA fiber diameter and alignment of tendon biomimetic fleece potentiate tenogenic differentiation and immunomodulatory function of amniotic epithelial stem cells. Cells 2020; 9(5): 1207.

21.

Zhou

, et al. Electrospun thymosin Beta-4 loaded PLGA/PLA nanofiber/microfiber hybrid yarns for tendon tissue engineering application. Mater Sci Eng C Mater Biol Appl 2020; 106(1): 110268.

22.

Yan

Meng

, et al. Double layer composite membrane for preventing tendon adhesion and promoting tendon healing. Mater Sci Eng C Mater Biol Appl 2021; 123(1): 111941.

23.

Chen

Yeh

. Alterations in connective tissue metabolism in stress incontinence and prolapse. J Urol 2011; 186(5): 1768–1772.

24.

Moon

Choi

Jeon

, et al. Alteration of elastin metabolism in women with pelvic organ prolapse. J Urol 2011; 185(5): 1786–1792.

25.

Vulic

Strinic

Tomic

, et al. Difference in expression of collagen type I and matrix metalloproteinase-1 in uterosacral ligaments of women with and without pelvic organ prolapse. Eur J Obstet Gynecol Reprod Biol 2011; 155(2): 225–228.

26.

Sun

Zhang

, et al. Adjustable hardness of hydrogel for promoting vascularization and maintaining stemness of stem cells in skin flap regeneration. Appl Mater Today 2018; 13(1): 54–63.

27.

Wang

Jiang

, et al. Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane. Int J Nanomed 2019; 14(14): 1835–1847.

28.

Han

Wong

, et al. Fibre-based scaffolding techniques for tendon tissue engineering. J Tissue Eng Regen Med 2018; 12(7): 1798–1821.

29.

Kendal

Snelling

Dakin

, et al. Resorbable electrospun polydioxanone fibres modify the behaviour of cells from both healthy and diseased human tendons. Eur Cel Mater 2017; 33(1): 169–182.

Supplementary Material

Please find the following supplemental material available below.

For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.

For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.

0.00 MB

0.03 MB