Glioma is the most common malignant tumor in the brain, accounting for over 80% of all primary intracranial tumors. The current clinical treatment has shown certain limitations. Although M1 type microglia can secrete various pro-inflammatory cytokines and are expected to be used for glioma treatment, direct use of microglia may lead to overactivation and trigger immune storms. Therefore, we first found that serum starvation can stimulate the transformation of microglia into M1 type. Subsequently, we found through comparative experiments that the inhibitory effect of microglial cell lysis medium on glioma cells was stronger than that of microglial cell culture medium. Finally, we successfully prepared sodium alginate hydrogel loaded with microglia lysis solution to achieve sustained inhibitory effect on the growth of glioma and avoid its proliferation.
Persaud-SharmaDBurnsJTrangleJ, et al.Disparities in brain cancer in the United States: a literature review of gliomas. Med Sci2017; 5(3): 16.
2.
ZhangJChenCLiA, et al.Immunostimulant hydrogel for the inhibition of malignant glioma relapse post-resection. Nat Nanotechnol2021; 16(5): 538–548.
3.
CanollPGoldmanJE. The interface between glial progenitors and gliomas. Acta Neuropathol2008; 116: 465–477.
4.
WellerMCloughesyTPerryJR, et al.Standards of care for treatment of recurrent glioblastoma—are we there yet?Neuro Oncol2013; 15(1): 4–27.
5.
LimMXiaYBettegowdaC, et al.Current state of immunotherapy for glioblastoma. Nat Rev Clin Oncol2018; 15(7): 422–442.
6.
HambardzumyanDGutmannDHKettenmannH. The role of microglia and macrophages in glioma maintenance and progression. Nat Neurosci2016; 19(1): 20–27.
7.
GinhouxFLimSHoeffelG, et al.Origin and differentiation of microglia. Front Cell Neurosci2013; 7: 45.
8.
SzulzewskyFPelzAFengX, et al.Glioma-associated microglia/macrophages display an expression profile different from M1 and M2 polarization and highly express Gpnmb and Spp1. PLoS One2015; 10(2): e0116644.
9.
HongSYouJYPaekK, et al.Inhibition of tumor progression and M2 microglial polarization by extracellular vesicle-mediated microRNA-124 in a 3D microfluidic glioblastoma microenvironment. Theranostics2021; 11(19): 9687–9704.
10.
LiYLiuZSongY, et al.M2 microglia-derived extracellular vesicles promote white matter repair and functional recovery via miR-23a-5p after cerebral ischemia in mice. Theranostics2022; 12(7): 3553–3573.
11.
LanzaMCasiliGCampoloM, et al.Immunomodulatory effect of microglia-released cytokines in gliomas. Brain Sci2021; 11(4): 466.
12.
ZhouWKeSQHuangZ, et al.Periostin secreted by glioblastoma stem cells recruits M2 tumour-associated macrophages and promotes malignant growth. Nat Cell Biol2015; 17(2): 170–182.
13.
GunassekaranGRPoongkavithai VadevooSMBaekMC, et al.M1 macrophage exosomes engineered to foster M1 polarization and target the IL-4 receptor inhibit tumor growth by reprogramming tumor-associated macrophages into M1-like macrophages. Biomaterials2021; 278: 121137.
14.
YaoYFuK-Y. Serum-deprivation leads to activation-like changes in primary microglia and BV-2 cells but not astrocytes. Biomed Rep2020; 13(5): 51.
15.
WuJGaoGShiF, et al.Activated microglia–induced neuroinflammatory cytokines lead to photoreceptor apoptosis in Aβ-injected mice. J Mol Med2021; 99: 713–728.
16.
ChengJ-NLuoWSunC, et al.Radiation-induced eosinophils improve cytotoxic T lymphocyte recruitment and response to immunotherapy. Sci Adv2021; 7(5): eabc7609.
17.
GuerraADYeungOWHQiX, et al.The anti-tumor effects of M1 macrophage-loaded poly (ethylene glycol) and gelatin-based hydrogels on hepatocellular carcinoma. Theranostics2017; 7(15): 3732–3744.
18.
LiXLiXYangJ, et al.In situ sustained macrophage-targeted nanomicelle–hydrogel microspheres for inhibiting osteoarthritis. Research2023; 6: 0131.
LuYWuCYangY, et al.Inhibition of tumor recurrence and metastasis via a surgical tumor-derived personalized hydrogel vaccine. Biomater Sci2022; 10(5): 1352–1363.
21.
LynnADBryantSJ. Phenotypic changes in bone marrow-derived murine macrophages cultured on PEG-based hydrogels activated or not by lipopolysaccharide. Acta Biomater2011; 7(1): 123–132.
22.
LiW, et al.Inflammatory Macrophages Lysate-based Macroporous Hydrogel for Effective Immunomodulation to Inhibit Tumor Progression, Recurrence and Metastasis2023.
23.
PanXYuanSXunX, et al.Long‐term recruitment of endogenous M2 macrophages by platelet lysate‐rich plasma macroporous hydrogel scaffold for articular cartilage defect repair. Adv Healthcare Mater2022; 11(6): 2101661.
24.
YangJ-SXieY-JHeW. Research progress on chemical modification of alginate: a review. Carbohydrate polymers2011; 84(1): 33–39.
25.
FanLCaoMGaoS, et al.Preparation and characterization of sodium alginate modified with collagen peptides. Carbohydr Polym2013; 93(2): 380–385.
26.
Vidal-ItriagoARadfordRAWAramidehJA, et al.Microglia morphophysiological diversity and its implications for the CNS. Front Immunol2022; 13: 997786.
27.
SeimonTTabasI. Mechanisms and consequences of macrophage apoptosis in atherosclerosis. J Lipid Res2009; 50 Suppl: S382–S387.
28.
GaoSBaiLJiaS, et al.Small extracellular vesicles of M1-BV2 microglia induce neuronal PC12 cells apoptosis via the competing endogenous mechanism of CircRNAs. Genes2022; 13(9): 1603.
29.
LiCWangYXingY, et al.Regulation of microglia phagocytosis and potential involvement of exercise. Front Cell Neurosci2022; 16: 953534.
30.
FuRShenQXuP, et al.Phagocytosis of microglia in the central nervous system diseases. Mol Neurobiol2014; 49: 1422–1434.
31.
StapplerEWaltonJDBeierS, et al.Abundance of secreted proteins of Trichoderma reesei is regulated by light of different intensities. Front Microbiol2017; 8: 2586.
32.
YinZRomanoAJManducaA, et al.Stiffness and beyond: what MR elastography can tell us about brain structure and function under physiologic and pathologic conditions. Top Magn Reson Imag2018; 27(5): 305–318.
33.
WangCSinhaSJiangX, et al.Matrix stiffness modulates patient-derived glioblastoma cell fates in three-dimensional hydrogels. Tissue Eng2021; 27(5-6): 390–401.
34.
StratouliasVVeneroJLTremblayMÈ, et al.Microglial subtypes: diversity within the microglial community. EMBO J2019; 38(17): e101997.
35.
BogieJFStinissenPHendriksJJ. Macrophage subsets and microglia in multiple sclerosis. Acta Neuropathol2014; 128: 191–213.
36.
LeeSHLeeJHOhEY, et al.Ethanol extract of Cnidium officinale exhibits anti-inflammatory effects in BV2 microglial cells by suppressing NF-κB nuclear translocation and the activation of the PI3K/Akt signaling pathway. Int J Mol Med2013; 32(4): 876–882.
37.
ChooYWKangMKimHY, et al.M1 macrophage-derived nanovesicles potentiate the anticancer efficacy of immune checkpoint inhibitors. ACS Nano2018; 12(9): 8977–8993.
38.
ZhouYWeymanCMLiuH, et al.IFN-γ induces apoptosis in HL-60 cells through decreased Bcl-2 and increased Bak expression. J Interferon Cytokine Res2008; 28(2): 65–72.
39.
BastiancichCDanhierPPréatV, et al.Anticancer drug-loaded hydrogels as drug delivery systems for the local treatment of glioblastoma. J Contr Release2016; 243: 29–42.
40.
LiuNWangXSteerCJ, et al.MicroRNA-206 promotes the recruitment of CD8+ T cells by driving M1 polarisation of Kupffer cells. Gut2022; 71(8): 1642–1655.
41.
ZhangH, et al.Effect of Raptor on invasion ability of glioma cells. Chin J Pathophysiol2014: 2280–2283.
