The combined antiviral effect of dextran sulphate (DS, MW 40000) and acyclovir (ACV) on herpes simplex virus type 1 (HSV-1) and Pseudorabies virus (PRV) in vitro and on experimental HSV-1 keratitis in rabbits, was studied. Dextran sulphate in combination with ACV results in synergistic effect, as measured by yield reduction assay and on herpes simplex keratitis in rabbits. The better therapeutic effect of the combination was proved by the decreased severity of ocular infection and the reduction of the virus shedding in tear film.
References
1.
AndreiG., and De ClercqE. (1990) Inhibitory effect of selected antiviral compounds on arenavirus replication in vitro. Antiviral Res14: 287–300.
2.
BabaM.PauwelsR.BalzariniJ.ArnoltJ.DesmyterJ., and De ClercqE. (1988a) Mechanism of inhibitory effect of dextran sulfate and heparin on replication of human immunodeficiency virus in vitro. Proc Natl Acad Sci, USA85: 6132–6136.
3.
BabaM.SnoeckR.PauwelsR., and De ClercqE. (1988b) Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes Simplex virus, cytomegalovirus, vesicular stomatitis virus, and human immunodeficiency virus. Antimicrob Agents Chemother32: 1742–1745.
4.
CrumpackerC.S.SchnipperL.E.MarloweS.I.KowalskyP.N.HersheyB.J., and LevinM.J. (1982) Resistance to antiviral drugs of herpes simplex virus isolated from a patient treated with acyclovir. N Engl J Med306: 343–346.
5.
ItoM.BabaM.SatoA.PauwelsR.De ClercqE., and ShigetaS. (1987) Inhibitory effect of dextran sulfate and heparin on the replication of human immunodeficiency virus (HIV) in vitro. Antiviral Res7: 361–367.
6.
MaudgalP.C.De ClercqE.DeschampsJ.MissottenL.De SomerP.BussonR.VanderhaegheH.VerhelstG.WalkerR.T., and JonesA.S. (1980) (E)-5-(2-Bromovinyl)-2′-deoxyuridine in the treatment of experimental herpes simplex keratitis. Antimicrob Agents Chemother17: 8–12.
7.
MitsuyaH.LooneyD.J.KunoS.UenoR.Wong-StaalF., and BroderS. (1988) . Dextran sulfate suppression of viruses in the HIV family: inhibition of virion binding to CD4+ cells. Science240: 646–649.
8.
SmithK.O.KennellW.L.PoirierR.H., and LyndF.T. (1980) In vitro and in vivo resistance of herpes simplex virus to 9-(2-hydroxyethoxymethyl) guanine (acycloguanosine). Antimicrob Agents Chemother17: 144–150.
9.
SpearP.G. (1985) Glycoproteins specified by herpes simplex virus. In the Herpes-viruses. RoizmanB. (ed.) New York: Plenum Press, pp. 315–356.
10.
TrousdaleM.D.DunkelE.C., and NesburnA.B. (1990) Effect of acyclovir on acute and latent herpes simplex virus infections in the rabbit. Invest Ophthalmol Vis Sci19: 1336–1341.
11.
TsaiC-C.FollisK.E., and YarnallM. (1990) Inhibitory effects of heterogeneous dextran sulphate compounds on simian type D retrovirus in vitro. Antiviral Chem Chemother1: 61–65.
12.
UenoR., and KunoS. (1987) Dextran sulfate, a potent anti-HIV agent in vitro having synergism with zidovudine. Lancetii: 1379.
