Analysis of short DNA fragments from Holocene peatmoss samples

Abstract

This paper describes our recent attempt to isolate and analyse DNA from old plant remains of the common peatmoss Sphagnum fuscum retrieved from a peat core collected in the mire Fuglmyra, in central Norway. DNA was recoverable and usable from subfossilized (10—450 years old) plant remains of the peatmoss. A chloroplast (trnL) and two nuclear (ITS2 and RAPDf) regions were co-amplified from 80 samples of different ages. The RAPDf region was the only variable one with three different haplotypes found among five samples. Comparison of the ancient sequences with modern sequences found in the extant population occurring at the same site ascertained a genetic link between modern and fossil samples of this species. This retrieval of ancient DNA from subfossilized moss remains isolated from peat cores has important implications for the palaeoecology of peatmosses by allowing direct estimates of plant population dynamics in space and time.

Keywords

Ancient DNA trnL ITS2 RAPDf Sphagnum peatlands population dynamics palaeoecology Norway Holocene.

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References

Barber, K.E. 1981: Peat stratigraphy and climatic change. AA Balkema.

Bennett, K.D. and Parducci, L. 2006: DNA from pollen: principles and potential. The Holocene 16, 1031-34.

Clymo, R.S. 1998: Sphagnum, the peatland carbon economy, and climate change . In Bates, J.W., Ashton, N.W. and Duckett, J.G., editors, Bryology for the twenty-first century. Manely Publishing and British Bryological Society, 361-68.

Cronberg, N. 1996: Clonal structure and fertility in a sympatric population of the peat mosses Sphagnum rubellum and Sphagnum capillifolium. Canadian Journal of Botany 74, 1375-85.

---- 2002: Colonization dynamics of the clonal moss Hylocomium splendens on islands in a Baltic land uplift area: reproduction, genet distribution and genetic variation. Journal of Ecology 90, 925-35.

Cronberg, N. , Rydgren, K. and kland, R.H. 2006: Clonal structure and genet-level sex ratios suggest different roles of vegetative and sexual reproduction in the clonal moss Hylocomium splendens. Ecography 29, 95-103.

Dongya, Y.Y. and Speller, C.F. 2006: Co-amplification of cytochrome b and D-loop mtDNA fragments for the identification of degraded DNA samples. Molecular Ecology Notes 6, 605-608.

Gugerli, F. , Parducci, L. and Petit, R.J. 2005: Ancient plant DNA: review and prospects. New Phytologist 166, 409-18.

Gunnarsson, U. , Shaw, A.J. and Lönn, M. 2007: Local-scale genetic structure in the peatmoss Sphagnum fuscum. Molecular Ecology 16, 305-12.

10.

Ohlson, M. and Dahlberg, B. 1991: Rate of peat increment in hummock and lawn communities on Swedish mires during the last 150 years. Oikos 61, 369-78.

11.

Pääbo, S. , Poinar, H. , Serre, D. , Jaenicke-Després, V. , Hebler, J. , Rohland, N. , Kuch, M. , Krause, J. , Vigilant, L. and Hofreiter, M. 2004: Genetic analyses from ancient DNA. Annual Reviews of Genetics 38, 645-79.

12.

Parducci, L. , Suyama, Y. , Lascoux, M. and Bennett, K.D. 2005: Ancient DNA from pollen: a genetic record of population history in Scots pine. Molecular Ecology 14, 2873-82.

13.

Shaw, A.J. , Cox, C.J. and Boles, S.B. 2003: Polarity of peatmoss (Sphagnum) evolution: who says bryophytes have no roots? American Journal of Botany 90, 1777-87.

14.

Solelm, T. 1986: Age, origin and development of blanket mires in Sør-Trøndelag, Central Norway. Boreas 15, 101-15.

15.

Stenøien, H. and Såstad, S.M. 1999: Genetic structure in three haploid peat mosses (Sphagnum) . Heredity 82, 391-400.

16.

Suyama, Y. , Kawamuro, K. , Kinoshita, I. , Yoshimura, Y. , Tsumura, Y. and Takahara, H. 1996: DNA sequence from a fossil pollen of Abies spp. from Pleistocene peat. Genes and Genetic Systems 71, 145-49.

17.

van der Velde, M. , During, H.J. , van de Zande, L. and Bijlsma, R. 2001: The reproductive biology of Polytrichum formosum: clonal structure and paternity revealed by microsatellites. Molecular Ecology 10, 2423-34.