Yellow gentian (Gentiana lutea L.) is a herbal species with a long-term use in traditional medicine due to its digestive and stomachic properties. This paper presents an investigation of the free radical scavenging activity of methanolic extracts of yellow gentian leaves and roots in two different systems using electron spin resonance (ESR) spectrometry. Assays were based on the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the superoxide radicals (O2+) generated by the xanthine/ xanthine oxidase (X/XO) system. The results of gentian methanolic extracts were compared with the antioxidant capacity of synthetic antioxidant butylated hydroxyanisole (BHA). This study proves that yellow gentian leaves and roots exhibit considerable antioxidant properties, expressed either by their capability to scavenge DPPH or superoxide radicals.
Van der Sluis WG , van Arkel J , Fischer FC , Labadie RP . Thin-layer chromatographic assay of photoactive compounds (furocoumarins) using the fungus Penicillium expansum as a test organism . J Chromatogr A1981; 214: 349-359 .
2.
Ariño A , Arberas I , Leiton MJ , Renobales M , Dominguez JB . The extraction of yellow gentian root (Gentiana lutea L.) . Zeitschr Lebens Untersuch Forsch1997; 205: 295-299 .
3.
Isiguro K , Yamaki M , Takagi S , Ikeda Y , Kawakami K , Ito K . Studies on iridoid-related compounds. IV. Antitumor activity of iridoid aglycones . Chem Pharm Bull1986; 34: 2375-2379 .
4.
Van der Sluis WG , van der Nat JM , Labadie RP . Thin-layer chromatographic bioassay of iridoid and secoiridoid glucosides with a fungitoxic aglucone moiety using b-glucosidase and the fungus Penicillium expansum as a test organism . J Chromatogr A1983; 259: 522-526 .
5.
Kondo Y , Takano F , Hojo H . Suppression of chemically and imunologically induced hepatic injuries by gentiopicroside in mice . Planta Med1994; 60: 414-416 .
6.
Szucs Z , Danos B , Nyiredy S . Comparative analysis of the underground parts of Gentiana species by HPLC with diode-array and mass spectrometric detection . Chromatographia2002; 56: S19S23 .
7.
Menković N , S<caron> avikin-Fodulović K , Savin K . Chemical composition and seasonal variations in the amount of secondary compounds in Gentiana lutea leaves and flowers . Planta Med2000; 66: 178-180 .
8.
Gonda R , Takeda T , Akiyama T . Studies on the constituents of Anaxagorea luzonensis A . Gray. Chem Pharm Bull2000; 48: 1219-1222 .
9.
Ashida S , Noguchi SF , Suzuki T . Antioxidative components, xanthone derivatives in Swertia japonica Makino . J Am Oil Chem Soc1994; 71: 1095-1099 .
10.
Bajpai MB , Asthana RK , Sharma NK , Chatterjee SK , Mukherjee SK . Hypoglycemic effect of swerchirin from the hexane fraction of Swertia chirayita . Planta Med1991; 57: 102-104 .
11.
Basnet P , Kadota S , Shimizu M , Namba T . Bellidifolin: a potent hypoglycemic agent in streptozotocin (STZ)-induced diabetic rats from Swertia japonica . Planta Med1994; 60: 507-511 .
12.
Chen SX , Wan M , Loh BN . Active constituents against HIV-1 protease from Garcinia mangostana . Planta Med1996; 62: 381-382 .
13.
Finnegan RA , Merkel KE , Patel JK . Constituents of Mammea americana L. XII: biological data for xanthones and benzophenones . J Pharm Sci1973; 62: 483-485 .
14.
Hase K , Kadota S , Basnet P , Li J , Takamura S , Namba T . Tetrahydroswertianolin: a potent hepatoprotective agent from Swertia japonica Makino . Chem Pharm Bull1997; 45: 567-569 .
15.
Sundaram BM , Gopalakrishnan C , Subramanian S , Shankaranarayanan D , Kameswaran L . Antimicrobial activities of Garcinia mangostana . Planta Med1983; 48: 5960 .
16.
Minami H , Kinoshita M , Fukuyama Y , Kodama M , Yoshizawa T , Sugiura M , Nakagawa K , Tago H . Antioxidant xanthones from Garcinia subelliptica . Phytochemistry1994; 36: 501-506 .
17.
Aust SD , Chignell CF , Bray TM , Kalyanaraman B , Mason RP . Free radicals in toxicology . Toxicol Appl Pharmacol1993; 120: 168-178 .
18.
Halliwell B . Mechanisms involved in the generation of free radicals . Pathol Biol1996; 44: 6-13 .
19.
Muramatsu H , Kogawa K , Tanaka M , Okumura K , Nishihori Y , Koike K , Kuga T , Niitsu Y . Superoxide dismutase in SAS human tongue carcinoma cell line is a factor defining invasiveness and cell motility . Cancer Res1995; 55: 6210-6214 .
20.
Das D , Bandyopadhyay D , Bhattacharjee M , Banerjee RK . Hydroxyl radical is the mayor causative factor in stress-induced gastric ulceration . Free Rad Biol Med1997; 23: 8-18 .
21.
Brown DR , Schmidt B , Kretzschmar HA . Role of microglia and host prion protein in neurotoxicity of a prion protein fragment . Nature1996; 380: 345-347 .
22.
Smith MA , Perry G , Riche Y , Sayre LM , Anderson VE , Beal MF , Kowall N . Oxidative damage in Alzheimer's . Nature1996; 382: 120-121 .
23.
Sies H . Strategies of antioxidant defense . Eur J Biochem1993; 215: 213-219 .
24.
Halliwell B , Aeschbach R , Löliger J , Aruoma OI . The characterization of antioxidants . Food Chem Toxicol1995; 33: 601-617 .
25.
Valentão P , Fernandens E , Carvalho F , Andrade PB , Seabra RM , Bastos ML . Hydroxyl radical and hypochlorous acid scavenging activity of small Centaury (Centaurium erythraea) infusion. A comparative study with green tea (Camellia sinensis) . Phytomedicine2003; 10: 517-522 .
26.
Valentão P , Fernandens E , Carvalho F , Andrade PB , Seabra RM , Bastos ML . Antioxidant activity of Centaurium erythraea infusion evidenced by its superoxide radical scavenging and xanthine oxidase inhibitory activity . J Agric Food Chem2001; 49: 3476-3479 .
27.
Lu Y , Foo LY . Antioxidant activities of polyphenols from sage (Salvia officinalis) . Food Chem2001; 75: 197-202 .
28.
Yamaguchi F , Ariga T , Yoshimura Y , Nakazawa H . Antioxidative and anti-glycation activity of garcinol from Garcinia indica fruit rind . J Agric Food Chem2000; 48: 180-185 .
29.
Kahl R . In Sies H ed. Oxidative stress: oxidants and antioxidants. Academic Press , 245-273.
30.
Nenadis N , Zafiropoulou I , Tsimidou M . Commonly used food antioxidants: a comparative study in dispersed systems . Food Chem2003; 82: 403-407 .
31.
Ko FN , Chu CC , Lin CN , Chang CC , Tehg CM . Isoorientin-6??-O-glucoside, a water-soluble antioxidant isolated from Gentiana arisanensis . Biochim Biophys Acta1998; 1389: 81-90 .
32.
Osman H , Nasarudin R , Lee SL . Extracts of cocoa (Theobroma cacao L.) leaves and their antioxidation potential . Food Chem2004; 86: 41-46 .
33.
Cetkovic GS , Djilas SM , Canadanovic-Brunet JM , Tumbas VT . Antioxidant properties of marigold extracts . Food Res Int2004; 37: 643-650 .
34.
Pazdzioch-Czochra M , Widenska A . Spectrofluorimetric determination of hydrogen peroxide scavenging activity . Anal Chim Acta2002; 452: 177-184 .
35.
Koide T , Noda H , Liu W , Ogata T , Kamada H . Determination of superoxide scavenging activity of a sample containing xanthine oxidase inhibitor by ESR spin trapping . Anal Sci2000; 16: 1029-1032 .
36.
Glatz Z , Pospísilová J , Musil P . Determination of gentiopicroside in extracts of Centaurium erythreae and Gentiana lutea by micellar electrokinetic capillary chromatography . J Liquid Chromatogr Rel Technol2000; 23: 1831-1839 .
37.
Calliste CA , Trouillas P , Allais DP , Simon A , Duroux JL . Free radical scavenging activities measured by electron spin resonance spectroscopy and B16 cell antiproliferative behaviours of seven plants . J Agric Food Chem2001; 49: 3321-3327 .
38.
Trouillas P , Calliste CA , Allais DP , Simon A , Marfak A , Delage C , Duroux JL . Antioxidant, anti-inflammatory and antiproliferative properties of sixteen water plant extracts used in the Limousin countryside as herbal teas . Food Chem2003; 80: 399-407 .
39.
Gao Z , Huang K , Yang X , Xu H . Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi . Biochim Biophys Acta1999; 1472: 643-650 .
40.
Ellnain-Wojtaszek M , Kruczynski Z , Kasprzak J . Investigation of the free radical scavenging activity of Ginko biloba L. leaves . Fitoterapia2003; 74: 1-6 .
41.
Kus <caron>ar A. Introduction of autochthonous populations of yellow gentian (Gentiana lutea L. subsp. symphyandra Murb.) into cultivation in Slovenia and analysis of harvested drug. Doctoral Dissertation, 2005: 95-95 p.
42.
Buffa M , Rosetti V , Lombard A , Sancin P , Bosia PD . Gentiana lutea L. fresh roots: variations in composition as a function of harvest location . Acta Pharm Jugoslav1991; 41: 6773 .
43.
Oh H , Ko EK , Kim DH , Jang KK , Park SE , Lee HS , Kim YC . Secoiridoid glucosides with free radical scavenging activity from the leaves of Syringa dilatata . Phytother Res2003; 17: 417-419 .
44.
He ZD , But PHP , Chan TWD , Dong H , Xu HX , Lau CP , Sun HD . Antioxidative glucosides from the fruits of Ligustrum lucidum . Chem Pharm Bull2001; 49: 780-784 .
45.
Owen RW , Mier W , Giacosa A , Hull WE , Spiegelhalder B , Bartsch H . Phenolic compounds and squalene in olive oils: the concentration and antioxidant potential of total phenols, simple phenols, secoiridoids, lignans and squalene . Food Chem Toxicol2000; 38: 647-659 .
46.
Sánchez GM , Re L , Giuliani A , Núñez-Sellés AJ , León-Fernández OS . Protective effects of Mangifera indica L. extract, mangiferin and selected antioxidants against tap-induced biomolecules oxidation and peritoneal macrophage activation in mice . Pharm Res2000; 42: 565-573 .
47.
Martínez G , Delgado R , Pérez G , Garrído G , Núnñez Selle Davison GP , Leós AJ , León OS . Evaluation of the in vitro antioxidant activity of Mangifera indica L. extract (Vimang) . Phytother Res2000; 14: 424-427 .
48.
Watanabe M , Ohshita Y , Tsushida T . Antioxidant compounds from buckwheat (Fagopyrum esculentum Möench) hulls . J Agric Food Chem1997; 45: 1039-1044 .
49.
Sato T , Kawamoto A , Tamura A , Tatsumi Y , Fujii T . Mechanism of antioxidant action of pueraria glycoside (PG)-1 (an isoflavonoid) and mangiferin (a xanthonoid) . Chem Pharm Bull1992; 40: 721-724 .
50.
Wanasundara UN , Shahidi F . Antioxidant and prooxidant activity of green tea extract in marine oils . Food Chem1998; 63: 335-342 .
