Reduction of asialo pathway gangliosides in Escherichia coli-activated peritoneal macrophages from endotoxin hyporesponsive mice

Abstract

Sialidase-sensitive gangliosides are reduced in endotoxin-activated macrophages from C3H/HeJ mice. Ganglioside variation in mice has been linked to the H-2 complex. The H-2 gene locus is the major histocompatibility complex of the mouse which encodes for major cell surface antigens as well as immune response genes (the Ir genes). To determine whether the ganglioside alteration is a direct pleotropic influence of the Lps defect or whether there is an indirect influence due to the H-2 haplotype, we examined some members of the BXH/Ty set of recombinant inbred mice. The BXH/Ty set was derived by a cross of the normal C75BU6J and the C3H/HeJ strain. The BxH-4/Ty strain was chosen as a hyporesponsive strain and the BxH-14/Ty strain for the normal counterpart. The normal parental strain, C57BI/6J, and the normal BxH-14/Ty strain show patterns consistent with the normal C3H/HeN strain. The reduction of sialidase-sensitive gangliosides (primarily GM1b (cisGM1)) occurs in the endotoxin-hyporesponsive strains, C3H/HeJ and BxH-4/Ty. The hyporesponsive C3H/HeJ parent strain and the normal BXH-14/Ty recombinant strain are H-2 k haplotype whereas the normal C57BI/6J parent strain and the hyporesponsive BXH-4/Ty recombinant strain are H-2 b haplotype. The ganglioside shifts are consistent with a major pleotropic effect of the Lps defect, not H-2 haplotype.

Get full access to this article

View all access options for this article.

References

Sweet M.J. , Hume D.A. Endotoxin signal transduction in macrophages. J Leukoc Biol 1996; 60: 8-26.

Raetz C.R.H. , Ulevitch R.J. , Wright S.D. , Sibley C.H. , Ding A. , Nathan C.F. Gram-negative endotoxin: an extraordinary lipid with profound effects on eukaryotic signal transduction. FASEB J 1991; 5: 2652-2660.

Sultzer B.M. , Castagna R. , Bandekar J. , Wong P. Lipopolysaccharide nonresponder cells: the C3H/HeJ defect. Immunobiology 1993; 187: 257-271.

Nakano M. , Shinomya H. The Lps mutational defect in C3H/HeJ mice. In: Morrison D.C. , Ryan J.L. (eds) Bacterial Endotoxic Lipopolysaccharides. Boca Raton: CRC Press, 1992; 311-328.

Rosenstreich D.L. Genetic control of endotoxin response: C3H/ HeJ mice. In: Berry L.J. (ed) Handbook of Endotoxin, vol 3. Cellular Biology of Endotoxin. Amsterdam: Elsevier, 1985; 82-122.

Rosenstreich D.L. , Vogel S.N. , Jacques A.R. , Wahl L.M. , Oppenheim J.J. Macrophage sensitivity to endotoxin: genetic control by a single codominant gene. J Immunol 1978; 121: 1664-1670.

Wiegandt H. The chemical constitution of gangliosides of the vertebrate nervous system . Behav Brain Res 1995; 66: 85-97.

Yohe H.C. , Coleman D.C. , Ryan J.L. Ganglioside alterations in stimulated murine macrophages. Biochim Biophys Acta 1985; 818: 81-86.

Yohe H.C. , Cuny C.L. , Berenson C.S. , Ryan J.L. Comparison of thymocyte and T lymphocyte gangliosides from C3H/HeN and C3H/HeJ mice. J Leukoc Biol 1988; 44: 521-528.

10.

Chaby R. , Morelec M-J. , Enserqueix D. , Girad R. Membrane glycolipid and phospholipid composition of lipopolysaccharide-responsive and -nonresponsive murine B lymphocytes. Infect Immun 1986; 52: 777-785.

11.

Yohe H.C. , Berenson C.S. , Cuny C.L. , Ryan J.L. Altered B-lymphocyte membrane architecture indicated by ganglioside accessibility in C3H/HeJ mice. Infect Immun 1990; 58: 2888-2894.

12.

Yohe H.C. , Cuny C. , Macula L.J. , Saito M. , McMurray W.J. , Ryan J.L. The presence of sialidase sensitive sialogangliotetraosyl ceramide (G M1b) in stimulated murine macrophages: deficiency of G M1b in Escherichia coli-activated macrophages from the C3H/HeJ mouse . J Immunol 1991; 146: 1900-1908.

13.

Illustrated Dictionary of Immunology. New York: CRC Press, 1995; 72.

14.

Furukawa K. , Roth S. , Sawicki J. Several galactosyltransferase activities are associated with mouse chromosome 17. Genetics 1986; 114: 983-991.

15.

Watson J. , Riblet R. , Taylor B.A. The response of recombinant inbred strains of mice to bacterial endotoxin . J Immunol 1977; 118: 2088-2093.

16.

Bailey D.W. Recombinant inbred strains. Transplantation 1971; 11: 325-327.

17.

Macala L.J. , Yohe H.C. In situ accessibility of murine macrophage gangliosides. Glycobiology 1995; 5: 67-75.

18.

Yohe H.C. , Ye S. , Reinhold B.B. , Reinhold V.N. Structural characterization of the disialogangliosides of murine peritoneal macrophages. Glycobiology 1997; In press.

19.

Bergeron A.J. , Yohe H.C. Reclassification of the BXH11/Ty mouse as an endotoxin-hyporesponsive strain. J Endotoxin Res 1996; 3: 141-144.

20.

Handbook on Genetically Standardized JAX® Mice, 5th edn. Bar Harbor: The Jackson Laboratory, 1997; 1-148.

21.

Hakomori S-I. Sphingolipid-dependent protein kinases. Adv Pharmacol 1996; 36: 155-171.

22.

Higashi H. , Yoshida S. , Sato K. , Yamagata T. Interaction of ganglioside with specific peptide sequences as a mechanism for the modulation of calmodulin-dependent enzymes. J Biochem 1996; 120: 66-73.

23.

Nakano M. , Terada Y. , Matsumura H. , Shinomiya H. Possible refractory site on LPS-induced interleukin 1 production in C3H/HeJ peritoneal macrophages. Adv Exp Med Biol 1990 ; 256: 347-359.