Nodular pyogranulomatous pneumonia associated with Besnoitia darlingi infection in a cat

Abstract

A 1-y-old, castrated male domestic shorthair cat was evaluated because of a 1-mo history of chronic cough, dyspnea, and lethargy that progressed to anorexia during the week preceding euthanasia. Radiographically, the right caudal and middle lung lobes were consolidated. Cytologic and histologic examination of the right caudal lung lobe revealed pyogranulomatous inflammation with intralesional protozoal organisms. Although morphologically similar to and immunohistochemically reactive for Toxoplasma gondii, the organisms had inconsistent features, such as variability in shape and size. With Sarcocystidae PCR assays for the 18S DNA and the ITS1 DNA markers and Sanger sequencing, we identified the organisms as Besnoitia darlingi. A comprehensive search of PubMed and Google Scholar revealed no prior reports, suggesting that feline pneumonia has not been reported previously in association with B. darlingi infection.

Keywords

Besnoitia darlingi cats lung pyogranuloma

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References

Bisby

, et al Sarcocystis sp. encephalomyelitis in a cat. Vet Clin Pathol 2010;39:105–112.

Dubey

Carpenter

JL.

Toxoplasma gondii-like schizonts in the tracheal epithelium of a cat. J Parasitol 1991;77:792–796.

Dubey

Carpenter

JL.

Unidentified Toxoplasma-like tissue cysts in the brains of three cats. Vet Parasitol 1993;45:319–321.

Dubey

, et al An unidentified Toxoplasma-like tissue cyst-forming coccidium in a cat (Felis catus). Parasitol Res 1992;78:39–42.

Dubey

, et al Structures of Toxoplasma gondii tachyzoites, bradyzoites, and sporozoites and biology and development of tissue cysts. Clin Microbiol Rev 1998;11:267–299.

Dubey

, et al Establishment of Besnoitia darlingi from opossums (Didelphis virginiana) in experimental intermediate and definitive hosts, propagation in cell culture, and description of ultrastructural and genetic characteristics. Int J Parasitol 2002;32:1053–1064.

Gondim

LFP

, et al Importance of serological cross-reactivity among Toxoplasma gondii, Hammondia spp., Neospora spp., Sarcocystis spp. and Besnoitia besnoiti. Parasitology 2017;144:851–868.

Houk

, et al Serological response of cats to experimental Besnoitia darlingi and Besnoitia neotomofelis infections and prevalence of antibodies to these parasites in cats from Virginia and Pennsylvania. J Parasitol 2011;97:259–261.

Lappin

, et al Cystoisosporiasis and other enteric coccidioses. In: Sykes

, ed. Greene’s Infectious Diseases of the Dog and Cat. 5th ed. Elsevier, 2023:1304.

10.

Olias

, et al Molecular pathology, taxonomy and epidemiology of Besnoitia species (Protozoa: Sarcocystidae). Infect Genet Evol 2011;11:1564–1576.

11.

Poitout

, et al Lung aspirate from a cat with respiratory distress. Vet Clin Pathol 1998;27:10.

12.

Portella

, et al Molecular detection of protozoa of the Sarcocystidae family in sheep from the State of Rio Grande do Sul, Brazil. Cienc Rural 2016;46:1613–1617.

13.

Tanhauser

, et al Multiple DNA markers differentiate Sarcocystis neurona and Sarcocystis falcatula. J Parasitol 1999;85:221–228.

14.

Verma

, et al Bobcats (Lynx rufus) are natural definitive host of Besnoitia darlingi. Vet Parasitol 2017;248:84–89.

15.

Zitzer

, et al Parasitemia due to Sarcocystis neurona-like infection in a clinically ill domestic cat. Vet Clin Pathol 2017;46:526–532.