In vitro digestibility study of thermal oxidized palm oleins Estudio de la digestibilidad in vitro de oleínas de palma termooxidadas

Abstract

Information on digestibility and absorption of oils and fats used for frying is under debate. To get knowledge on this, unused palm olein (9.27 ± 0.10% w/w polar content), used frying palm olein with a moderate degree of alteration (14.81 ± 0.90% w/w polar content) and highly altered used frying palm olein (26.36 ± 0.30% w/w polar content) and their respective nonpolar and polar fractions were studied. Samples were analyzed by high-performance size-exclusion chromatography before and after a 20-min in vitro incubation with pancreatic lipase. Formation of monoacylglycerols and free fatty acids reflected no relevant differences between unused and moderately altered oleins, whereas the most altered olein was hydrolyzed to a much lesser degree. The presence of oligomers (dimers and polymers of triacylglycerols) negatively affected the hydrolysis of triacylglycerol monomers in whole oleins. The hydrolysis of these monomers in the isolated nonpolar and polar fractions ranked between 60.2% and 78.5%. Oligomers were efficiently hydrolyzed by pancreatic lipase in whole un used and moderately altered oleins but not in the most altered one. Polymers from isolated polar fractions were poorly hydrolyzed or not hydrolyzed at all. These data suggest that whole oleins contained some compounds that increase susceptibility of oligomers to enzymatic hydrolysis and that such compounds were not present in the polar fraction.

Keywords

palm olein frying enzymatic hydrolysis pancreatic lipase digestibility triacylglycerol polymers and dimers

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References

Alexander J.F. (1966). Effect of diet handling on nutritional studies with used frying fats. Lipids 1: 254-257.

Arroyo R. (1995). Comportamiento de aceite de girasol y oleína de palma en frituras de patatas. Estudio in vitro de la actividad hidrolítica de la lipasa pancreática porcina sobre stistratos terntooxidados. Doctoral Thesis, Complutense University, Madrid, Spain.

Arroyo R. , Sánchez-Muniz F.J. , Cuesta C. , Burguillo F.J. and Sánchez-Montero J.M. (1996). Hydrolysis of used palm oil and sunflower oil catalysed by porcine pancreatic lipase. Lipids 31: 1133-1139.

Bottino N.R. (1962). Nutritive value of methyl linoleate and its thermal decomposition products. Journal of the American Oil Chemists' Society 39: 25-27.

Cuesta C. , Sánchez-Muniz F.J. and Varela G. (1988). Nutritive value of frying fats. In: Varela G. , Bender A.E. and Morton I.D. (eds.), Frying of Food. Principles, Changes, New Approaches. Chichester, UK: Ellis Horwood Ltd. pp. 141-144.

Dobarganes M.C. , Pérez-Camino M.C. and Gutiérrez González-Quijano R. (1984). Métodos analíticos de aplicación en grasas calentadas. I. Determinación de ésteres metílicos no alterados. Grasas y Aceites 35:172-177.

Dobarganes M.C. , Pérez-Camino M.C. and Márquez-Ruiz G. (1988). High-performance size-exclusion chromatography of polar compounds in heated and non-heated fat. Fat Science and Technology 90: 308-311.

Firestone D. (1996). Regulation of frying fat and oil. In: Perkins E.G. and Erickson M.D. (eds.), Deep Frying. Chemistry, Nutrition and Practical Applications. Champaign, IL: AO CS Press pp. 323-334.

Goh S.H. , Choo Y.M. and Ong S.H. (1985). Minor constituents of palm oil. Journal of the American Oil Chemists' Society 62: 237-240.

10.

González-Muñoz M.J. , Tulasne C. , Arroyo R. and Sánchez-Muniz F.J. (1996). Digestibility and absorption coefficients of palm olein—Relationships with thermal oxidation induced by potato frying. Fett/Lipid 98: 104-108.

11.

González-Muñoz M.J. , Bastida S. and Sánchez-Muniz F.J. (1998). Short term in vivo digestibility of triglyceride polymers, dimers, and monomers of thermoxidized palm olein used in deep-frying. Journal of Agriculture and Food Chemistry 46: 5188-5193.

12.

Kajimoto G. and Mukai K. (1970). Toxicity of rancid oil. IX. Digestibility of polymerized fatty acid in thermally oxidized soybean oil. Journal of the Japan Oil Chemistry Society 19: 66-70.

13.

Luddy F.E. , Barford R.A. , Herb S.F. , Magidman P. and Riemenschneider R.W. (1964). Pancreatic lipase hydrolysis of triglycerides by a semimicro technique. Journal of the American Oil Chemists' Society 41: 693-696.

14.

Márquez-Ruiz G. and Dobarganes M.C. (1996). Nutritional and physiological effects of used frying fats. In: Perkins E.G. and Erickson M.D. (eds.), Deep Frying. Chemistry, Nutrition and Practical Applications. Champaign, IL: AOCS Press pp. 160-182.

15.

Márquez-Ruiz G. , Guevel G. and Dobarganes M.C. (1998). Applications of chromatographic techniques to evaluate enzymatic hydrolysis of oxidized and polymeric triacylglycerols by pancreatic lipase in vitro. Journal of the American Oil Chemists' Society 75: 119-126.

16.

Márquez-Ruiz G. , Pérez-Camino M.C. and Dobarganes M.C. (1992). Digestibility of free fatty acids, dimers and polymers in the rat. Journal of the American Oil Chemists' Society 69: 930-934.

17.

Matsuchita S.J. (1975). Specific interactions of linoleic acid hydroperoxides and their secondary degraded products with enzyme proteins. Journal of Agriculture and Food Chemistry 23: 150-154.

18.

Miyashita K. , Tagaki T. and Frankel E.N. (1990). Preferential hydrolysis of monohydroperoxides of linoleoyl and linolenoyl triacylglycerol by pancreatic lipase. Biochimica Biophysica Acta 1045: 233-238.

19.

Nolen G.A. (1973). Feeding study of a used, partially hydrogenated soybean oil, frying fat in dog. Journal of Nutrition 103: 1248-1255.

20.

Parras M. (1996). La Demanda de Aceite de Oliva Virgen en el Mercado Español y las Posibilidades de Su Crecimiento: Un Enfoque de Marqueting Estratégico . Madrid: Ministerio de Agricultura, Pesca y Alimentación.

21.

Perrin J.L. , Perfetti P. , Dimitriades C. and Naudet M. (1985). Etude analytique approfondie d'huiles chauffées. I. Techniques analytiques et essais préliminaires. Revue Francaise des Corps Gras 32: 151-158.

22.

Romero A. (1998). Fritura de patatas y de alimentos congelados: Influencia del tipo de aceite y de la modalidad de fritura en la formación de compuestos de termoxidación y monómeros cíclicos. Doctoral Thesis, Complutense University, Madrid, Spain.

23.

Romero A. , Cuesta C. and Sánchez-Muniz F.J. (1998). Effect of oil replenishment during deep-fat frying of frozen foods in sunflower oil and high-oleic acid sunflower oil. Journal of the American Oil Chemists' Society 75: 161-167.

24.

Sánchez-Muniz F.J. , Cuesta C. and Garrido-Polonio M.C. (1993). Sunflower oil used for frying. Combination of column, gas and high-performance size-exclusion chromatography for its evaluation. Journal of the American Oil Chemists' Society 70: 235-240.

25.

Sánchez-Muniz F.J. and Sánchez-Montero J.M. (1999). Enzymatic methods for the study of thermally oxidized oils and fats. In: Boskou D. and Elmadfa I. (eds.), Frying of Food. Oxidation, Nutrient and Non-nutrient Antioxidants, Biologically Active Compounds and High Temperatures. Lancaster, Pennsylvania : Technomic Publishing, Inc. pp. 105-141.

26.

Waltking A.E. and Wessels H.J. (1981). Chromatographic separation of polar and non polar components on frying fats. Journal of the Association of Official Analytical Chemists 64: 1329-1330.

27.

Yuki E. and Ishikawa Y. (1976). Tocopherol contents of nine vegetable frying oils, and their changes under simulated deep-fat frying conditions. Journal of the American Oil Chemists' Society 53: 673-676.