The aim of this work was to test the feasibility of using ultrasonic measurements to estimate the fat content and identify the fat sources used in formulated dry-cured pork meat products. For that purpose, dry-cured sausages were prepared using different fat sources (two Iberian pork backfats, Iberian lard, and sunflower oil) and contents (fat content from 3% to 17% wet basis) and characterized by measuring the ultrasonic velocity (at 2 ℃, 6 ℃, 10 ℃, 15 ℃, 20 ℃, and 25 ℃), fatty acid profile, thermal behavior, and composition. The fatty acid composition affected the melting behavior of the sausages, which also involved different ultrasonic velocities, depending on the fat source used for the sausage formulation. Significant (p < 0.05) linear relationships were established between the ultrasonic velocity and the percentage of melted fat, by means of which the sausage batches were differentiated according to the fat source used. The ultrasonic velocity temperature dependence allowed the determination of the fat content (explained variance 96.1%) by measuring the ultrasonic velocity in the dry-cured sausages at 2 ℃ and 25 ℃ and using a semi-empirical equation. Therefore, the ultrasonic measurements could be considered as a reliable tool for the characterization and differentiation of formulated dry-cured meat products with different fat sources and contents.
Association of Official Analytical Chemist (1997) Official Methods of Analysis, 16th edn. Washington, DC: AOAC, International.
2.
BeneditoJCarcelJARosselloCMuletA (2001) Composition assessment of raw meat mixtures using ultrasonics. Meat Science57: 365–370.
3.
BeneditoJMuletAVelascoJDobarganesMC (2002) Ultrasonic assessment of oil quality during frying. Journal of Agricultural and Food Chemistry50: 4531–4536.
4.
CebulaDJSmithKW (1992) Differential scanning calorimetry of confectionery fats. Part II: effects of blends and minor components. Journal of the American Oil Chemists Society69: 992–998.
5.
CouplandJN (2004) Low intensity ultrasound. Food Research International37: 537–543.
6.
Fernández-CabanásVMPolvilloORodríguez-AcuñaRBotellaBHorcadaA (2011) Rapid determination of the fatty acid profile in pork dry-cured sausages by NIR spectroscopy. Food Chemistry124: 373–378.
7.
FolchJLeesMSloane-StanleyGH (1957) A simple method for the isolation and purification of total lipids from animal tissues. The Journal of Biological Chemistry226: 497–509.
8.
Garcia-GilNSantos-GarcésEMuñozIFulladosaEArnauJGouP (2012) Salting, drying and sensory quality of dry-cured hams subjected to different pre-salting treatments: skin trimming and pressing. Meat Science90: 389–392.
9.
HimawanCStarovVMStapleyAGF (2006) Thermodynamic and kinetic aspects of fat crystallization. Advances in Colloid and Interface Science122: 3–33.
10.
LlullPSimalSBeneditoJRosellóC (2002) Evaluation of textural properties of meat-based product (sobrassada) using ultrasonic techniques. Journal of Food Engineering53: 279–285.
11.
McClementsDJ (1997) Ultrasonic characterization of food and drinks: principles, methods and applications. Critical Reviews in Food Science and Nutrition37: 1–46.
12.
MendozaEGarcíaMLCasasCSelgasMD (2001) Inulin as fat substitute in low fat, dry fermented sausages. Meat Science57: 387–393.
13.
MuguerzaEAnsorenaDBloukasJGAstisaránI (2003) Effect of fat level and partial replacement of pork backfat with olive oil on the lipid oxidation and volatile compounds of Greek dry fermented sausages. Journal of Food Science68: 1531–1536.
14.
MuguerzaEFistaGAnsorenaDAstiasaranIBloukasJG (2002) Effect of fat level and partial replacement of pork backfat with olive oil on processing and quality characteristics of fermented sausages. Meat Science61: 397–404.
15.
MuguerzaEGimenoGAnsorenaAAstiasaránI (2004) New formulations for healthier dry fermented sausages: a review. Food Science and Technology15: 452–457.
16.
MuguerzaEGimenoOAnsorenaDBloukasJGAstiasaránI (2001) Effect of replacing pork backfat with pre-emulsified olive oil on lipid fraction and sensory quality of Chorizo de Pamplona – a traditional Spanish fermented sausage. Meat Science59: 251–258.
17.
NiñolesLClementeGVentanasSBeneditoJ (2007) Quality assessment of Iberian pigs through backfat ultrasound characterization and fatty acid composition. Meat Science76: 102–111.
18.
NiñolesLMuletAVentanasSBeneditoJ (2010) Ultrasonic assessment of the melting behaviour in fat from Iberian dry-cured hams. Meat Science85: 26–32.
19.
NiñolesLMuletAVentanasSBeneditoJ (2011) Ultrasonic characterisation of B. femoris from Iberian pigs of different genetics and feeding systems. Meat Science89: 174–180.
20.
NiñolesLSanjuanNVentanasSBeneditoJ (2008) Ultrasonic and sensory characterization of dry-cured ham fat from Iberian pigs with different genetics and feeding backgrounds. Meat Science80: 896–902.
21.
O'BrienRD (2009) Fats and Oils: Formulating and Processing for Applications, 3rd edn. USA: CRC Press, Taylor & Francis Group.
22.
Ortiz-SomovillaVEspaña-EspañaFGaitán-JuradoAJPérez-AparicioJDe Pedro-SanzEJ (2007) Proximate analysis of homogenized and minced mass of pork sausages by NIRS. Food Chemistry101: 1031–1040.
23.
Ortuño SánchezMF (2006) Manual práctico de aceites esenciales, aromas y perfumes. Spain: AIYANA.
24.
PapadimaSNBloukasJG (1999) Effect of fat level and storage conditions on quality characteristics of traditional Greek sausages. Meat Science51: 103–113.
25.
Pérez-PalaciosTAntequeraTDuránMLCaroARodríguezPG (2010) MRI-based analysis, lipid composition and sensory traits for studying Iberian dry-cured hams from pigs fed with different diets. Food Research International43: 248–254.
26.
PetrónMJMurielETimónMLMartínLAntequeraT (2004) Fatty acids and tryiacylglycerols profiles from different types of Iberian dry-cured hams. Meat Science68: 71–77.
27.
PoveyMJW (1997) Ultrasonic Techniques for Fluids Characterization. San Diego, CA, USA: Academic Press.
28.
PoveyMJWMcClementsDJ (1988) Ultrasonics in food engineering. Part I: introduction and experimental methods. Journal of Food Engineering8: 217–245.
29.
RubioBMartínezBSánchezMJGarcía-CachánMDRoviraJJaimeI (2007) Study of the shelf life of a dry fermented sausage “salchichón” made from raw material enriched in monounsaturated and polyunsaturated fatty acids and stored under modified atmospheres. Meat Science76: 128–137.
30.
Ruiz-CarrascalJVentanasJCavaRAndrésAIGarcíaC (2000) Texture and appearance of dry cured ham as affected by fat content and fatty acid composition. Food Research International33: 91–95.
31.
SandlerSRKaroW (1992) Source Book of Advances Organic Laboratory Preparations. San Diego: Academic Press.
32.
SantacatalinaJVGarcia-PerezJVCoronaEBeneditoJ (2011) Ultrasonic monitoring of lard crystallization during storage. Food Research International44: 146–155.
33.
Telis-RomeroJVáquiroHABonJBeneditoJ (2011) Ultrasonic assessment of fresh cheese composition. Journal of Food Engineering103: 137–146.
34.
ValenciaIAnsorenaDAstiasaránI (2006) Stability of linseed and antioxidants containing dry fermented sausages: a study of the lipid fraction during different storage conditions. Meat Science73: 269–277.
35.
VentanasSRuizJGarcíaCVentanasJ (2007) Preference and juiciness of Iberian dry-cured loin as affected by intramuscular fat content, crossbreeding and rearing system. Meat Science77: 324–330.
