The aim of this work was to study the fermentation process of honey with kefir grains through a comprehensive understanding of its rheological properties, probiotic cell viability, instrumental color parameters and kinetic aspects in a batch bioreactor and during storage. The results showed that kefir grains were well adapted to bioreactor conditions, reaching high levels of cell viability (over 106 CFU mL−1 for total yeast and bacteria), phenolic compounds content (190 GAE/100 g) and acidification after 24 h of fermentation at 30 ℃. Colorimetric analysis showed that lightness (L*) and redness (a*) remained constant, while yellowness intensities (b*) decreased during fermentation time. After 35 days of storage, honey kefir beverage maintained its chemical characteristics and microbial viability as required to be classified as a probiotic product. The Ostwald-de-Waele (R2 ≥ 0.98) and Herschel-Bulkley (R2 ≥ 0.99) models can be used to predict the behavior of honey kefir beverage. The parameters analyzed in this study should be taken into account for industrial production of this novel non-dairy beverage.
AlsayadiMMSJawfiYABelarbiMSabriFZ (2013) Antioxidant potency of water kefir. Journal of Microbiology, Biotechnology and Food Science2(6): 2444–2447.
2.
AlvesCCOResendeJVCruvinelRSVPradoMET (2008) Stability of the microstructure and carotenoids contents of the freeze-dried pequi (Caryocar brasiliense Camb.) pulp in the powdered form. Food Science and Technology28(4): 830–839.
3.
BelibagliKBDalgicAC (2007) Rheological properties of sour cherry juice and concentrate. Journal of Food Science and Technology42(6): 773–776.
4.
BoxEPHunterWGHunterJS (2005) Statistics for Experimenters: Design, Innovation and Discovery, New York, NY: Wiley.
5.
CastroAG (2003) Coord. In: Alves MMM (ed) A química e a reologia no processamento de alimentos, Lisbon, Portugal: Instituto Piaget, pp. 29–52.
6.
ChungCRojanasasitharaTMutilangiWMcClementsDJ (2016) Enhancement of colour stability of anthocyanins in model beverages by gum Arabic addition. Food Chemistry201(15): 14–22.
7.
Codex Alimentarius (2001) Revised Codex Standard for Honey. United Nations, Codex Stan 12. Rome, 1981.
8.
De Dea LindnerJPennaALBDemiateIMYamaguishiCTPradoMRMParadaJL (2013) Fermented foods and human health benefits of fermented functional foods. In: SoccolCRPandeyALarrocheC (eds) Fermentation processes engineering in the food industry, Boca Raton, FL: Taylor & Francis Inc, pp. 263–297.
9.
DiosmaGRomaninDERey-BuruscoMFLonderoAGarroteGL (2014) Yeasts from kefir grains: Isolation, identification, and probiotic characterization. World Journal of Microbiology and Biotechnology30(1): 43–53.
10.
FiordaFAPereiraGVMThomaz-SoccolVMedeirosAPRakshitSKSoccolCR (2016) Development of kefir-based probiotic beverages with DNA protection and antioxidant activities using soybean hydrolyzed extract, colostrum and honey. LWT – Food Science and Technology68(1): 690–697.
11.
FiordaFASoares JúniorMSDa SilvaFAGrosmannMVESoutoLRF (2013) Microestructure, texture and colour of gluten-free pasta made with amaranth flour, cassava starch and cassava bagasse. LWT – Food Science and Technology54(1): 132–138.
12.
Food and Agriculture Organization of the United Nations, World Health Organization [FAO/WHO] (2006) Probiotics in Food: Health and Nutritional Properties and Guidelines for Evaluation, Rome: Food and Agriculture Organization of the United Nations: World Health Organization.
13.
García-FontánMCMartínezSFrancoICarballoJ (2006) Microbiological and chemical changes during the manufacture of Kefir made from cows’ milk, using a commercial starter culture. International Dairy Journal16(1): 762–767.
14.
Güzel-SeydimZBGreeneAKBodineAB (2000) Determination of organic acids and volatile flavor substances A.C in kefir during fermentation. Journal of Food Composition Analysis13(1): 35–43.
15.
Huang Y, Cavinato AG and Rasco B. (2008). Fruit juice analysis. In: Sun DW (ed) Infrared Spectroscopy for Food Quality Analysis and Control. New York, NY: Academic Press, pp. 355–375.
16.
KayacierADoganM (2006) Rheological properties of some gums-salep mixed solution. Journal of Food Engineering72(3): 261–265.
17.
KristoEBiliaderisCGTzanetakisN (2003) Modelling of the acidification process and rheological properties of milk fermented with a yoghurt starter culture using response surface methodology. Food Chemistry83(3): 437–446.
18.
LannesSCSMedeirosMLGioielliLA (2004) Rheological properties of cupuassu and cocoa fats. Grasas y Aceites55(2): 115–121.
19.
LaureysDDe VuystL (2014) Microbial species diversity, community dynamics, and metabolite kinetics of water kefir fermentation. Applied and Environmental Microbiology80: 2564–2572.
20.
Lourens-HattinghAViljoenBC (2001) Growth and survival of a probiotic yeast in dairy products. Food Research International34(9): 791–796.
21.
MagallhãesKTDe Melo PereiraGVDiasDRSchwanRF (2010) Microbial communities and chemical changes during fermentation of sugary Brazilian kefir. World Journal of Microbiology and Biotechnology33(1): 1–10.
22.
MüllerHG (1973) Introduccion a la Reologia de los Alimentos, Zaragoza: Acribia, pp. 174.
23.
NishinoTShibahara-SoneHKikuchi-HayakawaHIshikawaF (2000) Transit of radical scavenging activity of milk products prepared by Maillard reaction and Lactobacillus casei strain Shirota fermentation through the hamster intestine. Journal of Dairy Science83: 915–922.
24.
PalR (2011) Rheology of simple and multiple emulsion. Current Opinion and Colloid and Interface Science16: 41–60.
25.
PelegrineDHSilvaSCGasparettoCA (2002) Rheological behavior of pineapple and mango pulps. LWT – Food Science and Technology35(8): 645–648.
26.
PennaALBRao-GurramSBarbosa-CánovasGV (2007) Effect of milk treatment on acidification, physicochemical characteristics, and probiotic cell counts in low fat yogurt. Milchwissenschaft62(1): 48–52.
27.
PlackettRLBurmanJP (1944) The design of optimum multifactorial experiments. Biometrika33: 305–325.
28.
PreedyVR (2014) Processing and Impact on Antioxidants in Beverages, 1st ed. Amsterdam: Academic Press, pp. 33–46.
29.
PuerariCMagalhãesKTSchwanRF (2012) New cocoa pulp-based kefir beverages: Microbiological, chemical composition and sensory analysis. Food Research International48(2): 634–640.
30.
RandazzoWCoronaOGuarcelloRFrancescaNGermanaMAErtenH (2016) Development of new non-dairy beverages from Mediterranean fruit juices fermented with water kefir microorganisms. Food Microbiology54(1): 40–51.
31.
RaoMA (1999) Rheology of Fluids and Semisolids: Principles and Applications, Gaitherburg, MD: An Publishers, Inc.
ShamsudinRDaudWRWTakrifMSHassanOIlicaliC (2009) Rheological properties of Josapine pineapple juice at different stages of maturity. International Journal of Food Science & Technology44(4): 757–762.
34.
SinghNIEipesonWE (2000) Rheological behaviour of clarified mango juice concentrates. Journal of Texture Studies31(3): 287–295.
35.
SingletonVRossiJA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture16(3): 144–158.
36.
Soccol CR, Fiorda FA, Prado MRM and Bellettini MB (2014) Bioprocesso para a produção de uma bebida fermentada a base de mel com propriedades probióticas. Instituto Nacional de Propriedade Industrial. Patente Brazilian Patent No. BR 102014021724 0.
37.
Statsoft (2007) STATISTICA 7.0 for Windows e Computer Program Manual, Tulsa: Statsoft, Inc.. CD Room.
38.
SteffeJF (1996) Rheological Methods in Food Process Engineering, (Chapter 1). 2nd ed. East Lansing, MI: Freeman Press, pp. 418.
39.
Tabilo-MunizagaGBarbosa-CánovasGV (2005) Rheology for the food industry. Journal of Food Engineering67(1): 147–156.
40.
ZaniratiSBFAbatemarcoMJrSandesSHCNicoliJRNunesACNeumannE (2015) Selection of lactic acid bacteria from Brazilian kefir grains for potential use as starter or probiotic cultures. Anaerobe32(1): 70–76.
41.
ZuritzCAMuñoz PuntesEMatheyHHPérezEHGascónARubioLA (2005) Density, viscosity and coefficient of thermal expansion of clear grape juice at different soluble solid concentrations and temperatures. Journal of Food Engineering71(2): 143–149.
