This work assesses the effect of Drypetes gossweileri essential oil on germination of Bacillus spores inoculated in orange juice and milk. We also report the capacity of the essential oil at 0.25, 0.5 and 1 µg/mL to induce sensitivity of spores to some irradiation treatments. The concentrations of essential oil were chosen after sensory analysis. The results show that the essential oil inhibited spores germination with minimal inhibitory concentrations inhibiting spore germination (MICg) of 10 µg/mL in the orange juice. In milk, the spores appeared to be less sensitive with MICg varying from 20 to 40 µg/mL. The sensory analysis revealed 2.5 µg/mL as the acceptable concentration of essential oil in orange juice. The essential oil at 1 µg/mL induced the sensitivity of spore of three Bacillus to alpha radiation of 101.4 Gy. Ultraviolet-C and microwave treatments in the presence of essential oil in general led to higher inactivation of spores up to 100% in some cases.
BanikSBandyopadhyaySGangulyS (2003) Bioeffects of microwave – A brief review. Bioresource Technology87: 155–159.
2.
BayoïJRDjouldeDRBogneKPNyegueMAVoundiOSBakaryD, et al.(2014) Activity of acetic acid on Bacillus stearothermophilus and Bacillus subtilis spores after sublethal pretreatments. International Journal of Innovative and Scientific Research10: 570–575.
3.
BelotiVBarrosMAFDe FreitasJCNeroLADe SouzaAJSantanaEHW, et al.(1999) Frequency of 2,3,5-triphenyltetrazolium chloride (TTC) non-reducing bacteria in pasteurized milk. Revista de Microbiologia30: 137–140.
4.
CelandroniFLongoITosorattiNRiannessiFGhepardiESolvettiS, et al.(2004) Effect of microwave radiation on Bacillus subtilis spores. Journal of Applied Microbiology94: 1220–1227.
5.
ChandrasekaranSRamanathanSBasakT (2013) Microwave food processing – A review. Food Research International52(1): 243–261.
6.
Dall'acquaFMarcianiSRodighieroG (1970) Inter-strand cross-linkages occurring in the photoreaction between psoralen and DNA. Federation of European Biochemical Societies Letters9(2): 121–123.
7.
DaryaeiHBalasubramaniamaVM (2013) Kinetics of Bacillus coagulans spore inactivation in tomato juice by combined pressure heat treatment. Food Control30: 168–175.
8.
Etoa F-X. (1985). Variation de la thermorésistance de la spore bactérienne (Bacillus stearothermophilus) en cours de germination. Thèse de Doctorat 3ème Cycle. Université Pierre et Marie Curie, France.
9.
European Union. (2009). List of member States’ authorizations of food and food ingredients which may be treated with ionizing radiation. Official Journal of the European Union C 283, 24 November.
10.
FriedbergECWalkerGCSiedeWWoodRDSchultzRAEllenbergerT (2006) DNA repair and mutagenesis, Washington: ASN Press.
11.
GiulianiRBevilacquaACorboMRSeveriniC (2010) Use of microwave processing to reduce the initial contamination by Alicyclobacillus acidoterrestris in a cream of asparagus and effect of the treatment on the lipid fraction. Innovative Food Science and Emerging Technologies11: 328–334.
12.
HeyndrickxM (2011) The importance of endospore-forming bacteria originating from soil for contamination of industrial food processing. Applied Environmental Soil Science2011: 1–11.
13.
HyldgaardMMygindTMeyerRL (2012) Essential oils in food preservation: Mode of action, synergies, and interactions with food matrix components. Frontiers of Microbiology3: 1–12.
14.
Ji-Hyoung H, Hyun-Joo K and Sang-Do H. (2012). Effect of combined radiation and NaOCl/ultrasonication on reduction of Bacillus cereus spores in rice. Radiation Physics and Chemistry 81: 1177–1180.
15.
JuvenBJKannerJSchvedFWeisslowiczH (1994) Factors that interact with the antibacterial action of thyme essential oil and its active constituents. Journal of Applied Bacteriology76: 626–631.
16.
KoJAKimWYParkHJ (2012) Effects of microencapsulated allylisothiocyanate (AITC) on the extension of the shelf-life of Kimchi. International Journal of Microbiology153: 92–98.
17.
MaldonadoMCAbanMPNavarroAR (2013) Chemical and essential oil effect on Alicyclobacillus acidoterrestris viability. Brazilian Journal of Microbiology44(4): 1133–1137.
18.
MarklandSMFarkasDFKnielKEHooverDG (2013) Pathogenic psychrotolerant sporeformers: An emerging challenge for low-temperature storage of minimally processed foods. Foodborne Pathogens and Diseases10: 413–419.
19.
MoellerRDoukiTCadetJStackebrandtENicholsonWLRettbergP, et al.(2007) UV-radiation-induced formation of DNA bipyrimidine photoproducts in Bacillus subtilis endospores and their repair during germination. International Microbiology10: 39–46.
20.
RajkovicAUyttendaeleMVermeulenAAndjelkovicMFitz-JamesIIn’t VeldP, et al.(2008) Heat resistance of Bacillus cereus emetic toxin, cereulide. Letter of Applied Microbiology46: 536–541.
21.
RibeiroWMNociFCroninDALyngJGMorganDJ (2009) Shelf life and sensory evaluation of orange juice after exposure to thermo sonication and pulsed electric fields. Food and Bioproducts Processing87: 102–107.
22.
Serrano-PérezJJLez-LuqueRGMercháNMSerrano-AndréSL (2008) The family of furocoumarins: Looking for the best photosensitizer for phototherapy. Journal of Photochemistry and Photobiology A: Chemistry199: 34–41.
23.
SilvaFVMGibbsPA (2010) Non proteolytic Clostridium spores in low acid distributed foods and design of pasteurization process. Trends Food Technology21: 95–105.
24.
SmithPAStewartJFyfeL (2001) The potential application of plant essential oils as natural food preservatives in soft cheese. Food Microbiology18: 463–470.
25.
TassouCDrosinosEHNychasG-JE (1995) Effects of essential oil from mint (Menthapiperita) on Salmonella enteritidis and Listeria monocytogenes in model food systems at 4 degrees and 10 degrees C. Journal of Applied Bacteriology78: 593–600.
26.
United States Food and Drug Administration. (2002). Ultraviolet radiation for the processing and treatment of food. Code of Federal Regulations, 21 (Part 179.39). Silver Spring, MD: United States Food and Drug Administration.
27.
VoundiOSNyegueMLazarIRaducanuDNdoyeFStamateM, et al.(2015) Effect of essential oils on germination and growth of some pathogenic and spoilage spore-forming bacteria. Foodborne Pathogens and Diseases12: 551–559.
28.
VoundiOSNyegueMBognePBayoïJEtoaF-X (2014) Activity of conessine at various temperatures and pH on inhibition of germination of Bacillus cereus and Bacillus stearothermophilus spores. African Journal of Microbiology Research8(4): 320–326.
29.
ZhangZJiangBLiaoXYiJHuXZhangY (2012) Inactivation of Bacillus subtilis spores by combining high-pressure thermal sterilization and ethanol. International Journal of Food Microbiology160: 99–104.
