Reactive oxygen species are implicated in the pathogenesis of cardiac hypertrophy. Haem oxygenase-1 (HO-1), the rate-limiting enzyme in haem catabolism, is induced by oxidative stress and confers protection against oxidative tissue injuries. We used Northern blotting to examine expression of HO-1 and heat shock protein 70 (HSP70) in the hypertrophic cardiac muscle of eight patients (one infant and seven children) who underwent surgery for congenital heart disease. Levels of HO-1 and HSP70 mRNA were significantly increased in all specimens, but the orders of magnitude of the increases were different, suggesting that the genes expressing HO-1 and HSP70 are regulated separately.
SuzukiTTakahashiTYamasakiAFujiwaraTHirakawaMAkagiR: Tissue-specific gene expression of heme oxygenase-1 (HO-1) and non-specific delta-aminolevulinate synthase (ALAS-N) in a rat model of septic multiple organ dysfunction syndrome. Biochem Pharmacol2000; 60: 275–283.
4.
AkagiRTakahashiTSassaS: Fundamental role of heme oxygenase in the protection against ischemic acute renal failure. Jpn J Pharmacol2002; 88: 127–132.
5.
TakahashiTAkagiRShimizuHHirakawaMSassaS: Heme oxygenase-1: a major player in the defense against oxidative tissue injury. In: Heme Oxygenase in Biology and Medicine. (AbrahamNGAlamJNathKA, eds). New York: Kluwer Academic/Plenum Publishers, 2002; pp 387–398.
6.
YachieANiidaYWadaTIgarashiNKanedaHTomaT: Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency. J Clin Invest1999; 103: 129–135.
7.
PossKDTonegawaS: Reduced stress defense in heme oxygenase 1-deficient cells. Proc Natl Acad Sci U S A1997; 94: 10925–10930.
8.
TodaNTakahashiTMizobuchiSFujiiHNakahiraKTakahashiS: Tin chloride pretreatment prevents renal injury in rats with ischemic acute renal failure. Crit Care Med2002; 30: 1512–1522.
9.
ShimizuHTakahashiTSuzukiTYamasakiAFujiwaraTOdakaY: Protective effect of heme oxygenase induction in ischemic acute renal failure. Crit Care Med2000; 28: 809–817.
10.
OdakaYTakahashiTYamasakiASuzukiTFujiwaraTYamadaT: Prevention of halothane-induced hepatotoxicity by hemin pretreatment: protective role of heme oxygenase-1 induction. Biochem Pharmacol2000; 59: 871–880.
11.
XieZKometianiPLiuJLiJShapiroJIAskariA: Intracellular reactive oxygen species mediate the linkage of Na+/K+-ATPase to hypertrophy and its marker genes in cardiac myocytes. J Biol Chem1999; 274: 19323–19328.
12.
NakamuraKFushimiKKouchiHMiharaKMiyazakiMOheT: Inhibitory effects of antioxidants on neonatal rat cardiac myocyte hypertrophy induced by tumor necrosis factor-alpha and angiotensin II. Circulation1998; 98: 794–799.
13.
TanakaKHondaMTakabatakeT: Redox regulation of MAPK pathways and cardiac hypertrophy in adult rat cardiac myocyte. J Am Coll Cardiol2001; 37: 676–685.
14.
DateMOMoritaTYamashitaNNishidaKYamaguchiOHiguchiY: The antioxidant N-2-mercaptopropionyl glycine attenuates left ventricular hypertrophy in in vivo murine pressure-overload model. J Am Coll Cardiol2002; 39: 907–912.
15.
NakamuraKIrieHFujisawaEYoshiokaHNinomiyaYSakumaI: Heat shock protein 72 expression in the right ventricle of patients undergoing congenital cardiac surgery. Acta Med Okayama2000; 54: 103–109.
16.
ChomczynskiPSacchiN: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem1987; 162: 156–159.
17.
MatsumiMTakahashiTFujiiHOhashiIKakuRNakatsukaH: Increased heme oxygenase-1 gene expression in the livers of patients with portal hypertension due to severe hepatic cirrhosis. J Int Med Res2002; 30: 282–288.
18.
TakahashiSTakahashiTMizobuchiSMatsumiMMoritaKMiyazakiM: Increased cytotoxicity of carbon tetrachloride in a human hepatoma cell line overexpressing cytochrome P450 2E1. J Int Med Res2002; 30: 400–405.
19.
YoshidaTBiroPCohenTMullerRMShibaharaS: Human heme oxygenase cDNA and induction of its mRNA by hemin. Eur J Biochem1988; 171: 457–461.
20.
WuBHuntCMorimotoR: Structure and expression of the human gene encoding major heat shock protein HSP70. Mol Cell Biol1985; 5: 330–341.
21.
TyrrellRMBasu-ModakS: Transient enhancement of heme oxygenase 1 mRNA accumulation: a marker of oxidative stress to eukaryotic cells. Methods Enzymol1994; 234: 224–235.
22.
StockerRYamamotoYMcDonaghAFGlazerANAmesBN: Bilirubin is an antioxidant of possible physiological importance. Science1987; 235: 1043–1046.
