Expression of CD34,α-Smooth Muscle Actin and Transforming Growth Factor-β1 in Squamous Intraepithelial Lesions and Squamous Cell Carcinoma of the Cervix
Free accessResearch articleFirst published online March, 2009
Expression of CD34,α-Smooth Muscle Actin and Transforming Growth Factor-β1 in Squamous Intraepithelial Lesions and Squamous Cell Carcinoma of the Cervix
This retrospective study investigated CD34, α-smooth muscle actin (α-SMA), and transforming growth factor-β1 (TGF-β1) expression in stromal cells of cervical intraepithelial neoplasias (CINs; n = 30), invasive cervical squamous cell carcinomas (SCCs; n = 38) and adjacent normal cervix. Normal cervix and CINs contained diffuse CD34-positive stromal cells but no α-SMA-positive myofibroblasts. In contrast, 34 of 38 SCCs were free of CD34-positive stromal cells and all contained α-SMA-positive stromal myofibroblasts; adjacent normal tissue contained CD34-positive stromal cells and no α-SMA-positive myofibroblasts. More intense TGF-β1 expression was observed in SCC cells than in normal cervical epithelium or CINs. This study shows that the disappearance of CD34-positive stromal cells and appearance of α-SMA-positive stromal myofibroblasts may be associated with transformation of cervical CIN to SCC. These findings support the suggestion that over-production of TGF-β1 in SCC cells is one potential mechanism mediating the transformation of stromal cells to myofibroblasts in cervical carcinogenesis.
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