We report a frequency domain optical tomography system utilizing three RF modulation frequencies, which are optimized for probing breast lesions of different size located at different depths. A real-time co-registered ultrasound scanner is used to provide on-site estimation of lesion size and location. Based on the lesion information, an optimal light modulation frequency can be selected, which may yield more accurate estimates of lesion angiogenesis and hypoxia. Phantom experiments have demonstrated that a high modulation frequency, such as 350Mhz, is preferable for probing small lesions closer to the surface while a low modulation frequency, such as 50Mhz, is desirable for imaging deeper and larger lesions. A clinical example of a large invasive carcinoma is presented to demonstrate the application of this novel technique.
References
1.
ChanceB.Near-infrared Images Using Continuous, Phase-modulated, and Pulsed Light with Quantitation of Blood and Blood Oxygenation. Ann. NY Acad. Sci.838, 29–45 (1998).
2.
TrombergB., ShahN., LanningR., CerussiA., EspinozaJ., PhamT., SvaasandL., and ButlerJ.Non-Invasive In Vivo Characterization of Breast Tumors Using Photon Migration Spectroscopy. Neoplasia2, 26–40 (2000).
3.
NtziachristosV., YodhA., SchnallM., ChanceB.Concurrent MRI and Diffuse Optical Tomography of Breast After Indocyanine Green Enhancement. PNAS97, 2767–2772 (2000).
4.
PogueB., PoplackS. P., McBrideT. O., WellsW. A., OstermanK., OsterbergU., and PaulsenK. D.Quantitative Hemoglobin Tomography with Diffuse Near-infrared Spectroscopy: Pilot Results in the Breast. Radiology218, 261–266 (2001).
5.
CulverJ. P., ChoeR., HolbokeM. J., ZubkovL., DurduranT., SlempA., NtziachristosV., ChanceB., YodhA. G.Three-dimensional Diffuse Optical Tomography in the Parallel Plane Transmission Geometry: Evaluation of a Hybrid Frequency Domain/Continuous Wave Clinical System for Breast Imaging. Med. Phys.30, 235–247 (2003).
6.
JiangH., XuY., IftimiaN., EggertJ., KloveK., BaronL., and FajardoL.Three-dimensional Optical Tomographic Imaging of Breast in a Human Subject. IEEE Trans. on Medical Imaging20, 1334–1340 (2001).
7.
TaroniP., SpinelliL., TorricelliA., PifferiA., DanesiniG. M., CubedduR. P.Multi-wavelength Time Domain Optical Mammography. Technology in Cancer Research and Treatment527–538 (2005).
8.
DehghaniH., PogueB., PoplackS. P., and PaulsenK. D.Multiwavelength Three-dimensional Near-infrared Tomography of the Breast: Initial Simulation, Phantom, and Clinical Results. Appl. Opt.42, 135–145 (2003).
9.
ZhangQ., BrukilacchioT. J., LiA., StottJ. J., ChavesT., HillmanE., WuT., ChorltonM. A., RaffertyE., MooreR. H., KopasD. B., BoasD.Co-registered Tomographic X-ray and Optical Breast Imaging: Initial Results. Journal of Bomedical Optics10, 024033 (2005).
10.
HefferE., PeraV., SchutzO., SieboldH., FantiniS.Near-infrared Imaging of the Human Breast: Complementing Hemoglobin Concentration Maps with Oxygenation Images. Journal of Biomedical Optics9, 1152–1160 (2004).
11.
GrosenickD., MoestaK. T., WabnitzH., MuckeJ., StroszczynskiC., MacdonaldR., SchlagP. M., and RinnebergH.Time-domain Optical Mammography: Initial Clinical Results on Detection and Characterization of Breast Tumors. Applied Optics42, 3170–3186 (2003).
12.
FloeryD., HelbichT. H., RiedlC. C., JaromiS., WeberM., LeodolterS., and FuchsjaegerM. H.Characterization of Benign and Malignant Breast Lesions with Computed Tomographic Laser Mammography (CTLM). Investigative Radiology40, 328–335 (2005).
13.
ChanceB., NiokaS. J., ZhangJ., ConantE. F., HwangE., BriestS., OrelS. G., Schnall, CzernieckiB. J.Breast Cancer Detection Based on Incremental Biochemical and Physiological Properties of Breast Cancers: A Six-Year, Two-Site Study. Acad Radiol23, 925–933 (2005).
14.
PiaoD. Q., JiangS., SrinivasanS., DehghaniH., and PogueB.Video-rate Near-infrared Optical Tomography using Spectrally Encoded Parallel Light Delivery. Optics Letters30, 2593–2595 (2005).
15.
GulsenG., XiongB., BirgulO., NalciogluO.Design and Implementation of a Multifrequency Near-infrared Diffuse Optical Tomography System. Journal of Biomedical Optics11, 014020, 1–10 (2006).
16.
ChenN. G., GuoP. Y., YanS. K., PiaoD. Q., and ZhuQ.Simultaneous Near Infrared Diffusive Light and Ultrasound Imaging. Appl. Opt.40, 6367–6380 (2001).
17.
ChenN. G., HuangM. M., XiaH. J., PiaoQ. Z., CroninE. B., and ZhuQ.Portable Near Infrared Diffusive Light Imager for Breast Cancer Detection. Journal of Biomedical Optics, 504–510 (2004).
18.
ZhuQ., HuangM. M., ChenN. G., ZarfosK., JagjivanB., KaneM., HegdeP., KurtzmanS. H.Ultrasound-guided Optical Tomographic Imaging of Malignant and Benign Breast Lesions. Neoplasia.5, 379–388 (2003).
19.
ZhuQ., CroninE., CurrierA., VineH. S., HuangM. M., ChenN. G., XuC.Benign Versus Malignant Breast Masses: Optical Differentiation Using US to Guide Optical Imaging Reconstruction. Radiology237, 57–66 (2005).
20.
ZhuQ., KurtzmanS. H., HegdeP., TannenbaumS., KaneM., HuangM. M., ChenN. G., JagjivanB., ZarfosK.Utilizing Optical Tomography with Ultrasound Localization to Image Heterogeneous Hemoglobin Distribution in Large Breast Cancers. Neoplasia7, 263–270 (2005).
21.
FishkinJ. B., CoquozO., AndersonE. R., BrennerM., TrombergB.Frequency-domain Photon Migration Measurements of Normal and Malignant Tissue Optical Properties in a Human Subject. Applied Optics36, 10–20 (1997).
22.
ZhuQ., ChenN. G., PiaoD. Q., GuoP. Y., and DingX. H.Design of Near Infrared Imaging Probe with the Assistance of Ultrasound Localization. Appl. Opt.40, 3288–3303 (2001).
23.
JiangH., PaulsenK., OsterbergU., PogueB., PattersonM.Optical Image Reconstruction Using Frequency-domain Data: Simulations and Experiments. J. Opt. Soc. Am. A13, 253–266 (1996).
24.
O'LearyM., BoasD., ChanceB., YodhA.Experimental Images of Heterogeneous Turbid Media by Frequency-domain Diffusing-photon Tomography. Optics Letters20, 426–428 (1995).
MatsubayashiR., MatsuoY., EdakuniG., SatohT., TokunagaO. Osamu, and KudoS. ShoBreast Masses with Peripheral Rim Enhancement on Dynamic Contrast-enhanced MR Images: Correlation of MR Findings with Histologic Features and Expression of Growth Factors. Radiology217, 841–848 (2000).
