Inhibition of Root Caries Progression by an Antibacterial Adhesive

Abstract

A dentin primer containing the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) has been shown to penetrate and kill the bacteria in artificially demineralized dentin. We hypothesized that an experimental adhesive system, which incorporates the MDPB-containing primer, would be effective in inhibiting the progression of root caries in vitro. Artificial caries lesions were prepared by either an acid-gel or a Streptococcus mutans culture technique on the roots of extracted human teeth. The progression of these lesions after the application of the experimental or proprietary adhesive system was examined. Further demineralization was completely prevented by the experimental adhesive system, while lesions managed with the proprietary materials showed limited ability to inhibit further demineralization. We conclude that the experimental adhesive system can inhibit the progression of root-surface caries in vitro, through a combination of its antimicrobial activity and sealing of the demineralized dentin.

Get full access to this article

View all access options for this article.

References

Arends J, Düschner H, Ruben JL (1997). Penetration of varnishes into demineralized root dentine in vitro. Caries Res 31:201–205.

Billings RJ, Brown LR, Kaster AG (1985). Contemporary treatment strategies for root surface dental caries. Gerodontics 1:20–27.

Bowden GHW (1990). Microbiology of root surface caries in humans. J Dent Res 69:1205–1210.

Bradshaw DJ, Marsh PD (1998). Analysis of pH-driven disruption of oral microbial communities in vitro. Caries Res 32:456–462.

Brailsford SR, Shah B, Simons D, Gilbert S, Clark D, Ines I, et al. (2001). The predominant aciduric microflora of root-caries lesions. J Dent Res 80:1828–1833.

Imazato S (2003). Antibacterial properties of resin composites and dentin bonding systems. Dent Mater 19:449–457.

Imazato S, Torii M, Tsuchitani Y, McCabe JF, Russell RR (1994). Incorporation of bacterial inhibitor into resin composite. J Dent Res 73:1437–1443.

Imazato S, Kinomoto Y, Tarumi H, Torii M, Russell RR, McCabe JF (1997). Incorporation of antibacterial monomer MDPB into dentin primer. J Dent Res 76:768–772.

Imazato S, Imai T, Russell RRB, Torii M, Ebisu S (1998a). Antibacterial activity of cured dental resin incorporating the antibacterial monomer MDPB and an adhesion-promoting monomer. J Biomed Mater Res 39:511–515.

10.

Imazato S, Ehara A, Torii M, Ebisu S (1998b). Antibacterial activity of dentine primer containing MDPB after curing. J Dent 26:267–271.

11.

Imazato S, Torii Y, Takatsuka T, Inoue K, Ebi N, Ebisu S (2001). Bactericidal effect of dentin primer containing antibacterial monomer methacryloyloxydodecylpyridinium bromide (MDPB) against bacteria in human carious dentin. J Oral Rehabil 28:314–319.

12.

Imazato S, Walls AWG, Kuramoto A, Ebisu S (2002). Penetration of an antibacterial dentine-bonding system into demineralized human root dentine in vitro. Eur J Oral Sci 110:168–174.

13.

Imazato S, Kinomoto Y, Tarumi H, Ebisu S, Tay FR (2003). Antibacterial activity and bonding characteristics of an adhesive resin containing antibacterial monomer MDPB. Dent Mater 19:313–319.

14.

Lynch E (1996). Antimicrobial management of primary root carious lesions: a review. Gerodontology 13:118–129.

15.

Miyazaki M, Onose H, Iida N, Kazama H (2003). Determination of residual double bonds in resin-dentin interface by Raman spectroscopy. Dent Mater 19:245–251.

16.

Ozaki K, Matsuo T, Nakae H, Noiri Y, Yoshiyama M, Ebisu S (1994). A quantitative comparison of selected bacteria in human carious dentine by microscopic counts. Caries Res 28:137–145.

17.

Schaeken MJ, Keltjens HM, Van Der Hoeven JS (1991). Effects of fluoride and chlorhexidine on the microflora of dental root surfaces and progression of root-surface caries. J Dent Res 70:150–153.

18.

Schüpbach P, Osterwalder V, Guggenheim B (1996). Human root caries: microbiota of a limited number of root caries lesions. Caries Res 30:52–64.

19.

Wallace MC, Retief DH, Bradley EL (1993). The 48-month increment of root caries in an urban population of older adults participating in a preventive dental program. J Public Health Dent 53:133–137.

20.

Wicht MJ, Haak R, Lummert D, Noack MJ (2003). Treatment of root caries lesions with chlorhexidine-containing varnishes and dentin sealants. Am J Dent 16:25A–30A.