Altered glial glutamate transporter expression in descending circuitry and the emergence of pain chronicity

Animals

Adult male Sprague–Dawley rats (Envigo-Harlan), ≈eight-week-old at the time of inoculation were used in all experiments. Rats were on a 12-h light/dark cycle and received food and water ad libitum. To produce inflammation and hyperalgesia, CFA (0.05 ml, Sigma) suspended in an oil:saline (1:1) emulsion was injected subcutaneously into the lateral plantar surface of one (behavioral and immunocytochemical studies) or two (Western blot studies) hind paws. The CFA injection produced an intense tissue inflammation of the hind paw characterized by erythema, edema, and hyperalgesia.^40–42 Since we were not studying the effect of adjuvant per se and vehicle injection may also produce an effect,^43,44 we used naïve animals as a control. The animals were housed in cages in which the floor was covered with soft bedding materials to minimize discomfort due to contact with a cold, hard surface. The inflamed animals groom normally and display normal locomotor activity. They maintain their weight, explore their environment, and interact with other rats. The behavioral studies involved stimulation that produced only momentary pain and the rats could escape from these stimuli at any time. The experiments were approved by the Institutional Animal Care and Use Committee of the University of Maryland School of Dentistry.

Behavioral testing

All behavioral tests were conducted under blind conditions. For thermal nocifensive responses, rats were placed in clear plastic chambers on an elevated table and allowed to acclimate for approximately 15 to 30 min. Thermal hyperalgesia was assessed by measuring the latency of paw withdrawal in response to a radiant heat source. ⁴⁵ The heat stimulus was applied from underneath the glass floor with a high-intensity projector lamp bulb (8 V, 50 W; Osram, Berlin, Germany) and focused on the plantar surface of the hind paw. The paw withdrawal latency (PWL) was determined by an electronic clock circuit. A 20-s cutoff was used to prevent tissue damage. The PWL was tested for three trials with 5-min intervals between each trial. The average of the three trials was then used for analysis.

For mechanical sensitivity, an electronic von Frey unit (EVF3, Bioseb) was used to deliver mechanical stimulation. The measurement range of the unit is 0 to 500 g with 0.1 g resolution. The hind paw skin was accessed through a dorsal approach as described previously ⁴⁶ except that an electronic von Frey probe was used. The probe was applied against the lateral edge of the hind paw with gradually increasing force. The force (g) that induced paw withdrawal was digitized by the unit and used as the threshold for mechanical nociception.

For assessing conditioned place avoidance (CPA) behavior,^47,48 animals were placed within a 40.5 × 30.5 × 16 cm Plexiglas chamber positioned on top of a mesh screen. One half of the chamber was painted white (light area) and the other half was painted black (dark area). During CPA behavior testing, animals were allowed unrestricted movement throughout the test chamber for the duration of a 30-min test period. Testing began immediately with a suprathreshold mechanical stimulus (758 mN von Frey monofilament) applied abruptly to the hind paw at 15-s intervals. The stimulus was applied to the CFA-injected paw when the animal was within the preferred dark area and to the non-injected side when the animal was within the non-preferred light area. Based on the location of the animal at each 15-s interval, the mean percentage of time spent in each side of the chamber was calculated for the entire test period. Rats normally prefer to stay in the dark area. However, if staying in the preferred dark side was associated with a painful and aversive stimulus, the rat was forced to decide to remain there or to go to the light area to avoid the painful stimulus. The tendency to move to and stay in the light area is a measure of the aversion of the stimulus. CFA induces an increase in time spent in the light area. Spending less time in the light area of the chamber would suggest reduced aversive behavior.

Paw thickness (mm) was measured with a digital caliper to assess edema after CFA.

Brainstem microinjections and targeted recombinant plasmid transfers by electroporation ⁴⁹

Rats were anesthetized with 2% to 3% isoflurane in a gas mixture of 30% O₂ balanced with 70% nitrogen and placed in a Kopf stereotaxic instrument (Mode 900, Kopf Instruments, Tujunga, CA). A midline incision was made after infiltration of lidocaine (2%) into the skin. A midline opening was made in the skull with a dental drill for inserting an injection needle into the target site. The coordinates for the RVM were as follows: 10.5 mm caudal to bregma, midline, and 9.0 mm ventral to the surface of the cerebellum. ⁵⁰ To avoid penetration of the transverse sinus, the incisor bar was set at 4.7 mm below the horizontal plane passing through the interaural line. Animals were subsequently maintained at ∼1% isoflurane. Microinjections (500 nl) were performed by delivering drug solutions slowly over a 10-min period using a Hamilton syringe with a 32-gauge needle. The sham group underwent identical procedures with the injection of the same volume of the vehicle.

For gene transfer, Suresilencing™ shRNA plasmids with green fluorescent protein (GFP) (SAB Bioscience) were used. The shRNA inserts are: GCAAGCGCTAAGAGAGTTCTT (GLT1), CTAGTAACTTGGCATCTGTAT (KBBP2), and GGAATCTCATTCGATGCATAC (scrambled control). For GLT1 overexpression, we transfected RVM with GLT1 plasmid with GFP expression (PcDNA3.1/Hrgro_GLT1 cloning site BAMH I and XhoI, Gift from Dr. J. Rothstein), and vectors without GLT1 were used as a control. Each vector (500 ng/500 nl) was firstly injected into the RVM. The injection needle was left in place for at least 15 min before being slowly withdrawn. A pair of Teflon coated silver positive and negative electrodes were placed along a line rostral and caudal to the injection site. For transfer of negatively charged plasmid into RVM neurons, seven square wave electric pulses (40 V, 50 ms, 1 Hz; model 2100; A-M Systems, Carlsborg, WA) were delivered. ⁵¹ All wound margins were covered with a local anesthetic ointment (Nupercainal; Rugby Laboratories), the wound was closed, and animals were returned to their cages after they recovered from anesthesia. Following behavioral testing, animals were anesthetized with pentobarbital and perfused with saline and 4% paraformaldehyde. Coronal sections (60 µm) of tissue sections stained with cresyl violet were examined to determine needle placement.

Western blot

The rats were anesthetized with isoflurane (3%) and quickly decapitated. A block of brainstem from post interaural line 1.3 mm to 2.5 mm was punched out and put immediately into dry ice. RVM tissues from the ventral half and middle portion of the rostral medulla ⁵⁰ were punched with a 15-gauge puncture needle. The tissues were homogenized in solubilization buffer (50 mM Tris.HCl, pH 8.0; 150 mM NaCl, 1 mM EDTA, 1% NP40, 0.5% deoxycholic acid, 0.1% sodium dodecyl sulfate (SDS), 1 mM Na₃VO₄, 1 U/ml aprotinin, 20 μg/ml leupetin, and 20 μg/ml pepstatin A). The homogenate was centrifuged at 20,200 g for 10 min at 4°C. The supernatant was removed. The protein concentration was determined using a detergent-compatible protein assay with a bovine serum albumin standard. Each sample contains proteins from one animal. The proteins (50 μg) were separated on a 4% to 20% SDS-polyacrylamide gel electrophoresis (PAGE) (Bio-Rad) and blotted to a nitrocellulose membrane (Amersham Biosciences). The blots were blocked with 5% milk in tris-buffered saline (TBS) buffer and then incubated with respective antibodies. The membrane was washed with TBS and incubated with horseradish peroxidase-linked secondary antibody. The immunoreactivity was detected using enhanced chemiluminescence (ECL, Amersham). In some experiments, the immunoreactivity was detected with near-infrared fluorescence. For the Odyssey Infrared Imaging System, 50 μg protein samples were denatured by boiling for 5 min and loaded onto 4% to 20% Bis-Tris gels (Invitrogene). After electrophoresis, proteins were transferred to nitrocellulose membranes. The membranes were blocked for 1 h with Odyssey Blocking Buffer and then incubated with primary antibodies diluted in Odyssey Blocking Buffer at 4°C overnight, followed by washing with phosphate-buffered saline (PBS) containing 0.1% Tween 20 (PBST) three times. The membranes were then incubated for 1 h with IRDye800CW-conjugated goat anti-rabbit IgG and IRDye680-conjugated goat anti-mouse IgG secondary antibodies (LI-COR Biosciences) diluted in Odyssey Blocking Buffer. The blots were further washed three times with PBST and rinsed with PBS. Proteins were visualized by scanning the membrane with 700- and 800-nm channels (Odyssey®CLx, LI-COR Biosciences). β-actin was used as a loading control.

Immunoprecipitation

Samples were incubated with anti-GLT1 antibody overnight and then with protein A/G-Sepharose beads (Santa Cruz Biotechnology). SDS sample buffer (0.05 ml) was added to elute proteins from the protein A/G beads. The eluant was separated on SDS-PAGE (7.5%) and transferred to a nitrocellulose membrane. The membranes were blocked and incubated with anti-nitrotyrosine antibody, further washed and incubated with anti-mouse IgG horseradish peroxidase (1:3,000), and ECL was performed. The membranes were then stripped and reprobed with anti-GLT1 antiserum.

Immunohistochemistry

Rats were deeply anesthetized with pentobarbital sodium (100 mg/kg, i.p.) and perfused transcardially with 4% paraformaldehyde in 0.1 M phosphate buffer at pH 7.4. The same block of caudal brainstem tissues as that for western blot was removed, post-fixed, and transferred to 25% sucrose (w/v) for cryoprotection. Free-floating tissue sections were incubated with relevant antibodies with 1% to 3% relevant normal sera, and single or double-labeling immunofluorescence was performed. Double-labeling immunofluorescence was performed with the secondary antibodies labeled with Cy2 (1:500, Jackson ImmunoResearch) or Alexa Fluor 488 (1:500, Invitrogen Molecular Probes) and Cy3 (1:500, Jackson ImmunoResearch) after incubation with respective primary antibodies. Control sections were processed with the same method except that the primary antisera are omitted or adsorbed by respective antigens.

Antibodies and drugs

The following reagents were purchased: mouse anti-rat GLT1/EAAT2 antibody (1:1,000, BD Transduction Laboratories), mouse anti-heterogeneous nuclear ribonucleoproteins (hnRNP)/KBBP2 antibody (1:2,000, Santa Cruz Biotechnology), rabbit anti-GLAST (EAAT1, 1:1,000, Alpha Diagnostic Intl), rabbit anti-EAAC1: neuronal glutamate transporter (EAAT3, 1:1,000, BD Transduction Laboratories); Rabbit anti-gamma-aminobutyric acid (GABA) Transporter 3 (GAT3, 1:1,000, EMD Millipore), rabbit anti-phospho-44/42 MAP kinase (Thr202/Thr204, 1:1,000, Cell Signaling), mouse anti-glial fibrillary acidic protein (GFAP, 1:2,000, Millipore), mouse anti-NeuN (1:1,000, Millipore), rabbit anti-GFP (1:1,000, Millipore), mouse anti-nitrotyrosine antibody (1:1,000, EMD Millipore), mouse anti-β-actin (1:3,000, Sigma-Aldrich), PD98059 (Mol wt: 267.28, Calbiochem), dihydrokainic acid (DHK, Mol wt: 215.25, Sigma-Aldrich), and morphine sulphate (Mol Wt: 758.83, Sigma-Aldrich). The drugs were dissolved in saline or 5% dimethyl sulfoxide (Sigma) and saline. The drug vehicle was used as a control. The doses of all agents were selected based on previous use in relevant studies.

Data analysis

Data are presented as mean ± S.E.M. For Western blot analysis, the images were digitized, and densitometric quantification of immunoreactive bands was carried out using UN-SCAN-IT gel (ver. 4.3, Silk Scientific Inc.). The relative protein levels were obtained by comparing the respective specific band to the β-actin control from the same membrane. The deduced ratios were further normalized to that of the naïve rats on the same membrane and illustrated as percentage of the naïve controls. Statistical comparisons were made by analysis of variance (ANOVA) followed by post hoc comparisons with Bonferroni corrections. For the animals that are subject to repeated testing, ANOVA with repeated measures is used with time as a within-animal effect. p ≤ 0.05 is considered significant for all cases.

Altered GLT1 expression in the RVM after hind paw inflammation

We first examined whether GLT1 expression altered in the RVM after CFA-induced hind paw inflammation. Total proteins from RVM tissues were isolated and separated. The GLT1 antibody recognizes one monomer band (≈ 65 kDa) and two oligomer (dimer and trimer) bands (Supplemental Figure 1).^22,52,53 Since GLT1 functions in the dimerized form,^20,38 we analyzed GLT1 dimer expression. As shown in Figure 1(a), GLT1 expression in the RVM exhibited biphasic changes after CFA. Compared to naïve animals, GLT1 was significantly increased initially at 30 min and gradually transitioned to downregulation after CFA. Downregulation of GLT1 reached significance at the two-week time point and remained at the significantly lower level throughout the eight-week observation period (p < 0.05). In naïve rats, GLT1 expression was not changed over the same period (Figure 1(b)), excluding an effect of other non-specific factors or aging on GLT1 expression.

OPEN IN VIEWER

Figure 1.

Altered glutamate transporter expression and GLT1 nitration in the RVM after hind paw inflammation. (a) Western immunoblot illustrating CFA-induced changes in dimerized GLT1 and GFAP expression. The RVM tissues were punched out at 30 min to eight weeks after CFA injection. Examples of the blot are shown on top and the relative protein levels are shown in the bottom histogram. Dashed line indicates the naïve level. The immunobands against β-actin were a loading control. (b) GLT1 expression in naïve rats over the same period as in (a). N = 4. (c) GLT1 immunostaining in the RVM. Py, pyramidal tract. (d) Double immunofluorescence labeling of GLT1 and GFAP illustrating astroglial localization of GLT1. Arrows indicate examples of GFAP and GLT1-labeled cells. The area in rectangles in top left and middle panels are enlarged below. (e) CFA-induced changes in GLAST and EAAC1 expression. *p < 0.05; **p < 0.01; ***p < 0.001 vs. naïve. N = 4 for each group. (f) CFA-induced changes in GLT-nitration. *p < 0.05 vs. naïve, N = 4. Error bars represent S.E.M. GLT1: glutamate type 1 transporter; GFAP: glial fibrillary acidic protein; CFA: complete Freund’s adjuvant.

Immunostaining with anti-GLT1 antibodies confirmed biphasic changes of GLT1 after CFA (Figure 1(c)) and colocalization of GLT1 with GFAP, an astroglial marker (Figure 1(d)). It is known that GLT1 expression does not mirror the level of GFAP. ⁵⁴ In contrast to GLT1, GFAP expression in the RVM was constantly upregulated during the eight-week observation period after inflammation (Figure 1(a)). The prolonged reactive astrocytosis indicates that downregulation of GLT1 is not due to loss or silence of local astrocytes. The other major glial EAAT, GLAST (EAAT1) showed a trend to increase at 30 min and transitioned to downregulation at two to eight weeks after CFA (Figure 1(e)). The neuronal glutamate transporter EAAC1 (EAAT3) showed upregulation at 30 min to five days after inflammation and returned to and maintained at the control level after one to two weeks (Figure 1(e)).

Reduced GLT1 activity is associated with its nitration by peroxynitrite.^55,56 Utilizing GLT1 immunoprecipitation and an anti-nitrotyrosine antibody, GLT1 nitration in the RVM showed a reduction at 30 min, no significant change at one week, and an increase at eight weeks after CFA (p < 0.05) (Figure 1(f)). Thus, the levels of GLT1 nitration showed an opposite pattern to GLT1, suggesting an initial transient increase and later decrease in GLT1 transporter activity after inflammation.

Re-evaluation of CFA-induced behavioral hyperalgesia

CFA-induced thermal hyperalgesia resolves in about two to four weeks ⁴⁰ (Figure 2(a)). We noticed that the downregulation of GLT1 occurred at a time when thermal pain hypersensitivity had started to attenuate or resolve. This seems to be against the hypothesis that reduced uptake of synaptic glutamate transmitter, as a result of GLT1 downregulation, leads to persistent neuronal hyperexcitability and hyperalgesia. We have performed experiments to address this apparent discrepancy.

OPEN IN VIEWER

Figure 2.

The time course of CFA-induced behavioral nociception. (a) Thermal hyperalgesia assessed with paw withdrawal responses to a noxious heat stimulus. (b) Mechanical hyperalgesia assessed with paw withdrawal threshold with an electronic von Frey probe. Open squares show PW threshold of a group of rats tested at longer intervals (N = 6). (a) and (b): *p < 0.05; **p < 0.01; ***p < 0.001 vs. baseline; ^##p < 0.01; ^###p < 0.001 vs. Contralateral. (c) The persistent pain hypersensitivity after CFA-induced inflammation was verified in a conditional place avoidance behavior test. Inflamed animals spend significantly more time in the light side of the chamber as compared to naïve rats, suggesting increased aversion, or negative affect, to the noxious stimulus. ***p < 0.001 vs. naïve. (d) The effects of morphine on long-lasting mechanical hyperalgesia. Morphine was injected i.p. at eight weeks after the injection of CFA (0.3 and 1 mg/kg, in 0.5 ml saline). Paw withdrawal threshold of the hind paw was significantly increased in both inflamed and non-inflamed paws at 45 to 60 min after the injection. *p < 0.05; ***p < 0.001 vs. saline; ^##p < 0.01; ^###p < 0.001 vs. 0.3 mg. N = 5–8. (e) Compared to saline, morphine (1 mg/kg) significantly reduced time spent in the light area of the testing chamber in the CPA behavior test, suggesting suppression of aversion. ***p < 0.001 vs. saline. N = 5–8. (f) Hind paw edema, as measured by the paw thickness, followed a similar course as behavioral hyperalgesia. ***p < 0.001 vs. baseline. (g) Comparison of body weight between CFA-injected and naïve rats. Naïve rats were weighed at the matching age points with CFA rats. *p < 0.05. CFA: complete Freund’s adjuvant.

Noting that CFA-induced mechanical hyperalgesia does not resolve as late as 3 weeks after inflammation, ⁴⁶ we directly compared the time courses of CFA-induced thermal and mechanical pain hypersensitivity up to 13 weeks after CFA. Consistent with the previous results, ⁴⁰ thermal hyperalgesia, indicated by shortened paw withdrawal latencies to a noxious heat stimulus, peaked at one day, started to attenuate at two days, and gradually resolved after about four weeks after CFA (Figure 2(a)). Mechanical allodynia/hyperalgesia showed a similar time course in the first three weeks as thermal hyperalgesia. However, mechanical hyperalgesia did not further attenuate from 2- to 3-week time point but persisted at a constant level throughout the 13-week observation period (Figure 2(b)). Thus, mechanical hyperalgesia exhibited initial developing (2 h to one day) and subsequent attenuating (three days to two weeks) phases and a late persistent phase (>two to four weeks) after inflammation. To exclude a possible sensitization of mechanical responses due to repeated testing, a group of rats was tested at longer intervals (N = 6). Similar long-lasting mechanical hyperalgesia was observed in rats receiving fewer tests (Figure 2(b), open squares). There was a trend of reduced PW threshold on the contralateral non-inflamed paw, although it only reached statistical significance at the one-day time point (Figure 2(b)). The late long-lasting behavioral nociception after CFA was sensitive to analgesics. At eight weeks after inflammation, morphine (0.3 and 1 mg/kg, i.p., p < 0.01) increased paw withdrawal threshold (PWT), indicating attenuation of hyperalgesia (Figure 2(d)).

The CFA-induced lasting pain hypersensitivity was also assessed with the CPA behavior test in separate sets of animals. As shown in Figure 2(c), naïve rats tended to avoid staying in the light area of the test chamber. They only spent a total of 20% ± 3% time in the light chamber. However, after receiving CFA, they spent over 60% of time in the light chamber (p < 0.001), indicating CFA-induced aversive behavior or pain affect. Different from mechanical hyperalgesia, there was no quantifiable difference in the magnitude of CPA behavior after CFA throughout the observation period. Morphine (1 mg/kg, i.p., p < 0.001) improved CPA behavior tested at eight weeks after inflammation (Figure 2(e)).

The paw edema as indicated by the paw thickness showed a time course similar to that of mechanical sensitivity with initial developing/attenuating phases and a late persistent phase (Figure 2(f)). CFA did not affect general growth assessed with body weight (Figure 2(g)). Compared to naïve rats, CFA-injected rats only showed reduced body weight at the 8-week time point during the 13-week period, suggesting that CFA did not have a general effect on the body.

Taken together, these results indicate that CFA-induced downregulation of GLT1 coincided with the emergence of the long-lasting pain hypersensitivity in the CFA model.

Altered GLT1 on pain sensitivity

We next examined whether inhibition of GLT1 in the RVM affect pain sensitivity. We first tested the effect of dihydrokainate (DHK), a GLT1 selective antagonist/glutamate uptake inhibitor. In naïve rats, intra-RVM injection (Figure 3(a)) of DHK (10 ng) produced a reduction of PWL in both hind paws (Figure 3(b)). Importantly, when injected at seven days after CFA when the GLT1 level and nitration were not significantly different from the naïve level (Figure 1(a) and (b)), DHK further reduced PWL on the inflamed paw and produced thermal hyperalgesia on the contralateral paw (Figure 3(c)).

OPEN IN VIEWER

Figure 3.

The effect of the GLT1 inhibitor on pain sensitivity. (a) Digital photomicrograph illustrating the site of microinjection in the RVM. The transverse section of the brainstem was taken at 10.30 mm posterior to the bregma. The start of penetration of the guide cannula is indicated by a block down arrow on top (middle). The tip of the microinjection needle is indicated by two small arrows dorsal to the pyramidal tract (py). 7, facial nerve; sp5, spinal trigeminal tract. (b) Intra-RVM injection of DHK reduced PWLs on both hind paws at 30 min after DHK injection in naïve rats. (c) At seven-day post-CFA, intra-RVM DHK further reduced PWL of the ipsilateral inflamed paw and induced hyperalgesia on the contralateral paw. *p < 0.05; **p < 0.01; ***p < 0.001 vs. Veh. DHK: dihydrokainic acid; CFA: complete Freund’s adjuvant.

We then focally knocked down GLT1 expression by RNAi in the RVM to mimic the tissue injury-induced reduction of GLT1 and examined its effect on nociception in normal animals. GLT1 shRNA plasmid was injected into the rat RVM and then effectively transferred into RVM cells by local electroporation. The same amount of unmatched shRNA plasmid was used as a control. Significant downregulation of GLT1 expression in the RVM was observed from one to five days after gene transfer, compared with the negative control shRNA (Figure 4(a) and (b)). Compared with the rats receiving control plasmid, GLT1 shRNA-treated animals showed significant decreases in PWLs (Figure 4(c)) and mechanical response threshold (Figure 4(d)) on both hind paws at one to seven days after gene transfer, indicating behavioral hyperalgesia. The pain hypersensitivity returned to the baseline level at 10 days after gene transfer. These results indicate that GLT1 activity in descending circuitry is involved in the modulation of nociceptive sensitivity.

OPEN IN VIEWER

Figure 4.

The effect of GLT1 knock down on nociception. (a) Tissue sections from the RVM were processed for GLT1 immunoreactivity from a rat receiving negative control shRNA (top) and a rat at three days after transfer of shRNA against GLT1 mRNA (bottom). Compared to the rat receiving negative control gene transfer, the GLT1 immunoreactivity was decreased in the RVM after the transfer of shRNA against GLT1 mRNA. (b) The GLT1 protein levels in the RVM were downregulated from one to five days and recovered at seven days after transfer of shRNA for GLT1 to the naïve rats. NC shRNA. *p < 0.05 vs. naïve. N = 4. (c) Both left and right PWLs were significantly decreased at one to seven days after the transfer of shRNA against GLT1 mRNA, compared to rats receiving negative control-shRNA (*p < 0.05 vs. Ctrl-shRNA). (d) The intra-RVM transfer of GLT1 shRNA decreased PW threshold of both paws. (c) and (d) Arrows indicate the gene transfection by electroporation. *p < 0.05; **p < 0.01; ***p < 0.001 vs. Ctrl-shRNA. GLT1: glutamate type 1 transporter; NC: negative control.

The effect of GLT1 overexpression on hyperalgesia

We next tested whether induced-GLT1 overexpression could reverse persistent phase hyperalgesia. At four weeks after the injection of CFA, GFP-GLT1 plasmid was transfected into the RVM through microinjection followed by electroporation. GFP-GLT1 expression was predominantly in GFAP-labeled astrocytes (Figure 5a (a–d)) but not in neurons (not shown). While thermal hyperalgesia was small in magnitude at this point, induced GLT1 expression led to an increase in PWLs in both hind paws after gene transfer (Figure 5(b), left). GLT1 overexpression also led to an increased mechanical response threshold on both hind paws (Figure 5(b), right). Notably, thermal and mechanical hyperalgesia on the inflamed paw were reversed (Figure 5(b)). These results provide behavioral evidence that downregulation of GLT1 expression in the RVM contributes to decreased descending pain inhibition or increased descending facilitation that led to the development of persistent pain.

OPEN IN VIEWER

Figure 5.

The effect of GLT1 overexpression on CFA-induced hyperalgesia. (A) GLT1-GFP immunostaining in the RVM. (a) Low power image of GLT1-GFP immunostaining. The area in the rectangle is enlarged in (b) to (d). (b) GLT1-GFP staining in high power. (c) GFAP immunostaining. (d) Double immunofluorescence labeling of GLT1-GFP and GFAP illustrating astroglial expression of transfected GLT1-GFP. (B) GLT1-GFP plasmid was transfected into the RVM at four weeks after CFA and its effect on behavioral hyperalgesia was assessed. GLT1 overexpression led to an increase in PWLs (Left) and PW threshold (Right) in both hind paws at five and seven days after gene transfer (Left). Note reversal of hyperalgesia on the inflamed paw. ***p < 0.001 vs. ctrl; ⁺p < 0.05; ⁺⁺⁺p < 0.001 vs. four-week post-CFA (Day 0). GLT1: glutamate type 1 transporter; GFAP: glial fibrillary acidic protein; GFP: green fluorescent protein.

Altered KBBP expression after hind paw inflammation

KBBP is a transcription factor that regulates GLT1 expression by binding to an essential element of the GLT1 promoter. ²⁵ We reasoned that KBBP expression would be similarly modulated by inflammation. We examined expression of KBBP in the RVM after hind paw inflammation. Western blot showed that KBBP expression was increased at 30 min to five days and returned to the baseline level at one week after injection of CFA (Figure 6(a)). Significant downregulation of KBBP was observed at two to eight weeks after inflammation (p < 0.05). Immunostaining of KBBP also showed an initial increase and later decrease of KBBP expression in the RVM (Figure 6(b)).

OPEN IN VIEWER

Figure 6.

Altered KBBP expression in the RVM after hind paw inflammation and the effect of KBBP knock down on GLT1 and nociception. (a) Western immunoblot illustrating CFA-induced changes in KBBP expression. The RVM tissues were punched out at 30 min to eight weeks after CFA injection. *p < 0.05 vs. naïve. N = 4 for each time point. Error bars represent S.E.M. (b) KBBP immunostaining in the RVM was increased at 30 min and decreased at two weeks after CFA injection. (c) The KBBP protein levels in the RVM were downregulated at three to five days and recovered at 14 d after transfer of KBBP-shRNA. NC shRNA. *p < 0.05 vs. naïve. N = 4. KBBP (d) and GLT1 (e) protein levels in the RVM were upregulated at 30 min after CFA. The upregulation of both KBBP and GLT1 was abolished after the KBBP-shRNA treatment. (f) The PW thresholds of both left and right hind paws were significantly decreased at 1 to 10 days after the transfer of KBBP-shRNA, compared to rats receiving negative control-shRNA. *p < 0.05; ***p < 0.001 vs. Ctrl-shRNA. CFA: complete Freund’s adjuvant; KBBP: kappa B-motif binding phosphoprotein; NC: negative control.

Similar upregulation of KBBP and GLT1 at the early 30-min time point prompted us to directly test whether KBBP contributed to CFA-induced GLT1. We injected shRNA against KBBP or negative control plasmid (1 μg) into the RVM, followed by electroporation. The treatment with KBBP shRNA reduced KBBP in the RVM at three to five days after transfection (Figure 6(c)). The control plasmid did not have an effect on KBBP expression. At three days after KBBP shRNA injection, CFA was injected into the hind paw. Both KBBP and GLT1 levels were unchanged at 30 min after CFA in KBBP shRNA-treated rats, while the control rats showed upregulation of KBBP and GLT1 (Figure 6(d) and (e)). Finally, the intra-RVM transfer of shRNA against KBBP mRNA produced pain hypersensitivity in naïve rats. PWT of both left and right hind paws was significantly decreased after the transfer of KBBP shRNA compared to rats receiving control plasmid (Figure 6(f)). These results suggest that KBBP regulates GLT1 expression after peripheral tissue injury and that KBBP-GLT1 activity contributes to descending pain modulation.

Initial upregulation of GLT1 contributes to delayed descending facilitation after inflammation

What is the functional significance of initial increase in GLT1 expression and activity after peripheral tissue injury? The upregulation of GLT1 and decreased GLT1 nitration occurred as early as 30 min after the injection of CFA (Figure 1(a) and (b)), which is consistent with neuronal input-dependent GLT1 activation.^25,57 KBBP was also upregulated at 30 min after CFA (Figures 6 (a) and 7(a)). To examine a role of injury-related afferent input in altered GLT1 expression, a local anesthetic lidocaine (2%, 0.3 ml) was injected subcutaneously into the rat hind paw prior to the injection of CFA. ⁵⁸ Ten minutes after the infiltration of lidocaine, CFA was injected into the same site. Compared to saline-treated rats, pretreatment with lidocaine prevented upregulation of GLT1 and KBBP (Figure 7(a)), suggesting that inflammation-induced GLT1/KBBP upregulation was dependent on input from the injured site. CFA induces rapid activation/phosphorylation of the extracellular signal-regulated kinase (ERK), mitogen-activated protein kinase (MAPK) in the RVM (Figure 7(b)). ⁵⁹ pERK appeared in RVM neurons, but not astroglial cells, after inflammation (Figure 7(c)). At 30 min after the injection of CFA, phosphor-ERK (P44/42) exhibited significant upregulation in the RVM (not shown). Intra-RVM pretreatment (10 min before CFA) with PD98059 (50 ng), a MAPK kinases inhibitor, blocked CFA-induced upregulation of pERK as well as upregulation of GLT1 at 30 min after CFA (Figure 7(d)), further confirming a role of neuronal activity in altered GLT1.

OPEN IN VIEWER

Figure 7.

Significance of early upregulation of GLT1. (a) The effect of preemptive local anesthesia on inflammation-induced increase in GLT1. Lidocaine (Lid, 0.3 ml, 2%) was injected into the plantar surface of the hind paw 10 min prior to CFA injection into the same site. Saline (Sal, 0.3 ml, 0.9%) was injected as a control for lidocaine. The upregulation of both GLT1 and KBBP was prevented by local anesthetic block. *p < 0.05 vs. naïve and lidocaine, N = 4. (b) CFA-induced activation of ERK MAPK in the RVM. Examples of immunofluorescence staining illustrating inflammation-induced increase in pERK in the RVM. CFA was injected into one hind paw and the rat was perfused at 30 min after CFA. (c) Double immunofluorescence staining shows that CFA-induced pERK colocalized with NeuN, a neuronal marker, but not with GFAP, an astroglial marker. Arrows indicate examples of double-labeled neurons. (d) Intra-RVM injection of PD98059 (PD, 50 ng), a MAPK kinases inhibitor, attenuated CFA-induced upregulation of pERK and GLT1. *p < 0.05 vs. naïve or Veh, N = 4. Intra-RVM injection of DHK (10 ng), a selective GLT1 inhibitor, produced a further reduction of PWL (e) and PW threshold (f) in the inflamed paw at 20 min after CFA. ***p < 0.001 vs. Veh. CFA: complete Freund’s adjuvant; GFAP: glial fibrillary acidic protein; DHK: dihydrokainic acid.

We then studied the effect of early pharmacological block of GLT1 on the development of pain hypersensitivity. Dihydrokainate (10 ng) was injected into the RVM at 10 min prior to the injection of CFA. At 20 min after CFA, rats receiving DHK showed significantly shorter PWLs and lower PW threshold (p < 0.001) (Figure 7(e) and (f)), suggesting earlier development of pain hypersensitivity. These results suggest that GLT1 expression is regulated by neuronal input. The increased GLT1 activity immediately after inflammation may serve to enhance glutamate uptake, which counteracts increased synaptic activity and dampens the development of hyperalgesia.