Sodium butyrate enhances intestinal integrity,inhibits mast cell activation,inflammatory mediator production and JNK signaling pathway in weaned pigs

Abstract

The present study aimed to investigate the effects of sodium butyrate on the intestinal barrier and mast cell activation, as well as inflammatory mediator production, and determine whether mitogen-activated protein kinase signaling pathways are involved in these processes. A total of 72 piglets, weaned at 28 ± 1 d age, were allotted to two dietary treatments (control vs. 450 mg/kg sodium butyrate) for 2 wk. The results showed that supplemental sodium butyrate increased daily gain, improved intestinal morphology, as indicated by greater villus height and villus height:crypt depth ratio, and intestinal barrier function reflected by increased transepithelial electrical resistance and decreased paracellular flux of dextran (4 kDa). Moreover, sodium butyrate reduced the percentage of degranulated mast cells and its inflammatory mediator content (histamine, tryptase, TNF-α and IL-6) in the jejunum mucosa. Sodium butyrate also decreased the expression of mast cell-specific tryptase, TNF-α and IL-6 mRNA. Sodium butyrate significantly decreased the phosphorylated ratio of JNK whereas not affecting the phosphorylated ratios of ERK and p38. The results indicated that the protective effects of sodium butyrate on intestinal integrity were closely related to inhibition of mast cell activation and inflammatory mediator production, and that the JNK signaling pathway was likely involved in this process.

Keywords

Sodium butyrate intestinal barrier mast cell inflammatory mediators mitogen-activated protein kinases weaned pigs

Introduction

Weaning is the most significant event in the life of pigs as they are abruptly forced to adapt to nutritional, immunological and psychological disruptions.¹ Stresses associated with early weaning usually result in growth retardation, post-weaning diarrhea and impaired intestinal barrier of piglets.^2–4 It is well known that sodium butyrate is involved in promoting growth, preventing diarrhea and restoring mucosal barrier integrity in piglets.^5,6 Generally speaking, the role of sodium butyrate in intestinal integrity is primarily owing to its ability to provide energy for intestinal epithelial cells.⁷ Recently, several studies have shown that sodium butyrate exerts anti-inflammatory effects in vitro.^8–10 However, whether the beneficial role of sodium butyrate in intestinal integrity of weaned pigs is related to alleviation of intestinal inflammation remains unknown. Moreover, the underlying mechanism also needs to be further investigated.

Intestinal mucosal mast cells reside in the lamina propria underneath the epithelium and play a key role in intestinal inflammation.^11,12 Mast cells contain large amounts of preformed compounds commonly referred to as mast cell inflammatory mediators, such as protease, histamine and cytokines.^13,14 Once the mast cells are activated, these mediators are released throughout gastrointestinal tracts and cause epithelial barrier dysfunction.^15–17 A few recent studies have reported that sodium butyrate inhibits mast cell activation and production of its mediator in vitro.^18,19 It would be of interest to investigate whether sodium butyrate influences mast cell activation in vivo. However, no data are available regarding the effect of sodium butyrate on mast cell activation in weaned pigs.

MAPK pathways transduce signals from a diverse array of extracellular stimuli.^20,21 Three principal members—ERK, p38 MAPK and c-Jun NH2–terminal kinase (JNK)—comprise this superfamily.²² Interestingly, Masuda et al.²³ and Zhang et al.¹⁹ reported that in murine bone marrow-derived mast cells, MAPK signaling pathways are involved in the production of mast cell inflammatory mediators; therefore, it is imperative that the effect of sodium butyrate on MAPK activation in weaned pigs is investigated.

Accordingly, we hypothesized that sodium butyrate would improve intestinal barrier function by influencing mast cell activation and inflammatory mediator production through modulation of MAPK signaling pathways. This study was conducted to assess the impact of sodium butyrate on intestinal barrier function and to determine whether MAPK signaling pathways are involved in the protective role of sodium butyrate on intestinal integrity.

Materials and methods

Animals and experiment design

The experiment was approved by the Animal Care and Use Committee of Zhejiang University. In a commercial farm, a total of 72 weaned piglets (Duroc × Landrace × Yorkshire), with an average initial mass of 8.5 kg weaned at 28 ± 1 d, were randomly assigned to two groups for 2 wk. Each group had six pens of six piglets. Based on previous studies, weaned piglets were fed a diet containing 0 or 450 mg/kg sodium butyrate from coated sodium butyrate. The coated sodium butyrate (containing 30% sodium butyrate) was produced and supplied by Shanghai Sinomenon Feed Co. Ltd. (Shanghai, China). Diets were formulated according to the National Research Council (1998) requirements (Table 1). Piglets were given ad libitum access to feed and water. Average daily gain (ADG), average daily feed intake and feed to gain ratio were calculated.

Table 1.

Ingredient and composition of diets on an as-fed basis.

Ingredients (g/kg)
Maize	343
Extruded corn	200
Soybean meal	105
Extruded full-fat soybean	100
Fish meal	30
Spray-dried plasma protein	30
Dried whey	80
Soybean oil	18
Dicalcium phosphate	10
Limestone	5
Sodium chloride	1
l-Lysine HCl	4.2
d,l-Methionine	1.8
Sucrose	25
Glc	25
Vitamin-mineral premix^a	20.5
Analysed composition	Total
Digestible energy^b (MJ/kg)	14.9
Crude protein	209.8
Lysine	14.7
Methionine	4.4
Calcium	8.1
Total phosphorus	6.8

Provided the following per kg of diet: vitamin A, 8000 IU; vitamin D, 2000 IU; vitamin E, 30 IU; vitamin K3, 1.5 mg; vitamin B1, 1.6 mg; vitaminB6, 1.5 mg; vitamin B12, 12µg; niacin, 20 mg; d-pantothenic acid, 15 mg; Zn, 80 mg; Fe, 100 mg; Cu, 20 mg; Mn, 25 mg; I, 0.3 mg; Se, 0.2 mg. ^bDigestible energy was calculated from data provided by Feed Database in China (2011).

Sample collection

After the feeding trial (d 14 post-weaning), six piglets from each treatment (one pig from every pen) were euthanized with a dose of sodium pentobarbital (200 mg/kg of body mass) according to Chen et al.²⁴ Specimens (1 cm) of the proximal jejunum were fixed in 10% formalin for morphology measurements. Adjacent specimens were prepared for Ussing chamber studies. The mucosa samples from remaining jejunum were collected, rapidly frozen in liquid nitrogen and stored at −80℃ until analysis.

Intestinal morphology

Segments for morphological study were embedded in paraffin, sectioned and stained with hematoxylin and eosin. Villus height and crypt depth were determined with an image processing and analysis system (Leica Imaging Systems, Cambridge, UK).

Ussing chamber experiments

Transepithelial electrical resistance (TER) and fluorescein isothiocyanate dextran 4 kDa (FD4) were measured in an Ussing chamber system. Segments of the jejunum were stripped from the seromuscular layer in oxygenated Ringer’s solution and then mounted in an EasyMount Ussing chamber system (model VCC MC6; Physiologic Instruments, San Diego, CA, USA) as described previously.⁴ In brief, the clamps were connected to Acquire and Analyse software (Physiologic Instruments) for automatic data collection. After a 15-min equilibration period on Ussing chambers, TER was recorded at 15-min intervals over a 1-h period. FD4 (Sigma-Aldrich, St. Louis, MO, USA) was added to the mucosal side at the final concentration of 0.4 mg/ml. The concentration of FD4 was measured by a fluorescence microplate reader (FLx800; BioTek Instruments, Winooski, VT, USA).

Mast cell counting

Sections of jejunum were prepared and then stained with toluidine blue. Sections were viewed at × 20 objective and data were presented as percentage of degranulated mast cells. The degranulated mast cells included mast cells that released > 50% of intracellular granules around the cell.²⁵ Mast cell counts were conducted on five different fields per slide and six slides per treatment. All cell counts were performed by at least two reviewers who were blinded to experimental treatments.

Mast cell inflammatory mediator analysis by ELISA

Jejunum mucosa was homogenized in PBS and the supernatant was collected. Samples were then diluted 1:10 in PBS and assayed for histamine, tryptase, TNF-α, IL-6 and IFN-γ using a commercial porcine ELISA assay (R&D Systems, Minneapolis, MN, USA).²⁶

mRNA expression analysis by real-time PCR

mRNA expression of mast cell-specific tryptase (MCT7), TNF-α, IL-6 and IFN-γ from jejunal mucosa was determined by quantitative real-time PCR, as described by Liu et al.²⁷ The primers used are given in Table 2. Briefly, total RNA was extracted from jejunal mucosa using TRIzol reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s guidelines. The purity and concentration of all RNA samples were measured using a Nano Drop spectrophotometer (ND-2000; NanoDrop Technologies, Wilmington, DE, USA). Reverse transcription using the PrimeScripte RT reagent kit (TaKaRa Biotechnology, Dalian, China) was carried out following the manufacturer’s instructions. Quantitative analysis of PCR was carried out on a StepOne Plus real-time PCR system (Applied Biosystems, Foster City, CA, USA) using SYBRGreen Master mix (Promega, Madison, WI, USA), according to the manufacturer’s instructions. Gene-specific amplification was determined by melting curve analysis and agarose gel electrophoresis. The 2^–ΔΔCt method was used to analyze the relative expression (fold changes), calculated relative to the values from the control group. The change (Δ) in Ct values in each group was compared with the Ct value of GAPDH (ΔCt). Subsequently, ΔΔCt was computed for each target gene from the treatment groups by subtracting the averaged_ΔCt for the control group. The final fold differences were computed as 2^–ΔΔCt for each target gene. The results showed that GAPDH exhibited no difference between two groups.

Table 2.

Primer sequences used for real-time PCR.

Primer name	Forward (5′-3′)	Reverse (5′-3′)
MCT7	CTGAGATGCCTCGACCAATAC	TCCGTTGACCTCGTCATAGTA
TNF-α	CATCGCCGTCTCCTACCA	CCCAGATTCAGCAAAGTCCA
IL-6	ATCAGGAGACCTGCTTGATG	TGGTGGCTTTGTCTGGATTC
IFN-γ	GAGCCAAATTGTCTCCTTCTAC	CGAAGTCATTCAGTTTCCCAG

Protein expression analysis by Western blot

The method for Western blot analysis was the same as the procedures outlined by Hu et al.⁴ In brief, after electrophoresis, the proteins were transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA, USA). The membranes were incubated with primary Ab at 4℃ for 12 h, then with the secondary Ab for 1 h at room temperature (25–27℃). The primary Abs [p38, phospho-p38, JNK, phospho-JNK (p-JNK), ERK, phospho-ERK (p-ERK)] and the secondary Ab (HRP-conjugated anti-rabbit Ab) were all purchased from Cell Signaling Technology (Danvers, MA, USA). Western blot was done with an enhanced chemiluminescence detection kit (Amersham, Arlington Heights, IL, USA), photographed by a ChemiScope 3400 (Clinx Science Instruments, Shanghai, China) and analyzed using Quantity One software. The values were calculated as the ratios of the phosphorylation levels and the total levels of MAPKs (JNK, p38, ERK).

Statistical analysis

Data were analyzed using SPSS 9.0 statistical package (IBM, Armonk, NY, USA). Results are expressed as mean ± SD. Differences between means were tested using Student’s t-test. Differences were considered significant at P < 0.05.

Results

Growth performance

The effects of sodium butyrate on growth performance of weaned piglets are shown in Table 3. Dietary supplementation with sodium butyrate significantly improved ADG compared with the control (P < 0.05).

Table 3.

Effects of sodium butyrate on growth performance of weaned pigs.

Items	Control	Sodium butyrate
Average daily gain	243.08 ± 8.87	257.13 ± 8.36^a
Average daily feed intake	306.71 ± 13.34	316.91 ± 25.73
Feed to gain ratio	1.26 ± 0.09	1.23 ± 0.10

Data are mean ± SD (n = 6).

Differences were considered significant at P < 0.05.

Intestinal morphology and barrier function

Intestinal morphology and barrier function are shown in Table 4. The weaned piglets fed with sodium butyrate had significantly higher (P < 0.05) villus height and villus height:crypt depth ratio at the jejunal mucosa compared with the control pigs. The TER and mucosal-to-serosal flux of FD4 in the Ussing chamber were used to assess the effects of sodium butyrate on the intestinal barrier function of pigs. The TER values were significantly increased (P < 0.05) and the FD4 fluxes were significantly decreased (P < 0.05) in the jejunum of weaned pigs supplemented with sodium butyrate compared with the control pigs.

Table 4.

Effects of sodium butyrate on intestinal morphology and barrier function in jejunum of weaned pigs.

Items	Control	Sodium butyrate
Villus height (µm)	394.02 ± 25.90	450.38 ± 23.34^a
Crypt depth (µm)	240.97 ± 21.04	219.42 ± 20.71
Villus heigh/crypt depth	1.64 ± 0.08	2.07 ± 0.25^a
TER (Ω.cm²)	51.52 ± 5.57	61.68 ± 6.37^a
FD4 flux (µg/cm²/h)	2.53 ± 0.24	2.07 ± 0.35^a

Data are mean ± SD (n = 6).

Differences were considered significant at P < 0.05.

Mast cell degranulation

Figure 1 shows the effects of sodium butyrate on mast cell degranulation in intestinal mucosa of piglets. In comparison with the control, dietary supplementation with sodium butyrate significantly reduced the percentage of degranulated mast cells (P < 0.05). However, the total mast cell numbers in jejunum mucosa showed no (P > 0.05) difference between the two groups.

Figure 1.

Effects of sodium butyrate on mast cell degranulation in jejunum mucosa of weaned pigs. Values are means ± SD (n = 6). *Differences were considered significant at P < 0.05.

Figure 2.

Effects of sodium butyrate on MAPK signal pathways in the jejunal mucosa of weaned pigs. The three MAPKs are JNK, p38 and ERK. The bands are representative blots from one of six pigs. The values are calculated as the ratios of their phosphorylation levels (p-JNK, p-p38, p-ERK) and the total levels of MAPKs. *Differences were considered significant at P < 0.05.

Mast cell inflammatory mediators content

Table 5 shows the effects of sodium butyrate on mast cell inflammatory mediator contents in intestinal mucosa of piglets. Compared with the control, dietary supplementation with sodium butyrate significantly reduced the content of histamine, tryptase, TNF-α and IL-6 in the jejunum mucosa (P < 0.05).

Table 5.

Effects of sodium butyrate on mast cell inflammatory mediators content in jejunum mucosa of weaned pigs.

Items	Control	Sodium butyrate
Histamine (ng/mg protein)	18.12 ± 3.79	11.17 ± 3.19^a
Tryptase (pg/mg protein)	35.83 ± 7.08	21.83 ± 5.49^a
TNF-α (pg/mg protein)	56.99 ± 10.58	31.55 ± 8.18^a
IL-6 (pg/mg protein)	22.53 ± 6.58	15.22 ± 3.22^a
IFN-γ (pg/mg protein)	53.69 ± 11.19	44.17 ± 8.66

Data are mean ± SD (n = 6).

Differences were considered significant at P < 0.05.

Intestinal mRNA expression of mast cell inflammatory mediators

The effects of sodium butyrate on mRNA relative expressions of inflammatory mediators in intestinal mucosa of piglets are shown in Table 6. Compared with the control, dietary supplementation with sodium butyrate significantly decreased the mRNA levels of MCT7, TNF-α and IL-6 in jejunal mucosa (P < 0.05).

Table 6.

Effect of sodium butyrate on relative mRNA expression of mast cell inflammatory mediators in jejunal mucosa of weaned pigs.^a

Items	Control	Sodium butyrate
MCT7	1.00 ± 0.32	0.49 ± 0.17^b
TNF-α	1.00 ± 0.41	0.36 ± 0.08^b
IL-6	1.00 ± 0.36	0.46 ± 0.15^b
IFN-γ	1.00 ± 0.25	0.71 ± 0.20

The 2-ΔΔCt method was used to analyze the relative expression (fold changes), calculated relative to the control group. Values are mean ± SD (n = 6).

Differences were considered significant at P < 0.05.

MAPK signaling pathways

Figure 1 shows the effects of sodium butyrate on the three MAPK signaling pathways (JNK, p38, ERK). In comparison with the control, supplemental sodium butyrate significantly decreased the phosphorylated ratio of JNK (p-JNK/JNK) (P< 0.05), whereas it did not significantly affect the phosphorylated ratios of ERK and p38 (p-ERK/ERK and p-p38/p38) (P > 0.05).

Discussion

Growth retardation is a common problem in weaned piglets. The present result, that dietary addition of sodium butyrate increased daily gain in weaned pigs, is consistent with previous research.²⁸ Moreover, sodium butyrate increased villus height and villus height:crypt depth ratio, which is consistent with the result of Lu et al.²⁹ The gastrointestinal tract is not only fundamental to the uptake of nutrients, but it also acts as a physical barrier. Disruption of the intestinal barrier facilitates luminal antigens to penetrate sub-epithelial tissues, resulting in a mucosal and systemic inflammatory response.^3,30 Therefore, we evaluated the effect of sodium butyrate on the intestinal barrier of weaned piglets, using the Ussing chamber technique. A decreased TER and increased FD4 flux reflect increased paracellular permeability and impaired intestinal barrier. The present study showed that pigs fed with sodium butyrate showed higher TER and lower FD4 flux in jejunum, which indicated an improvement in intestinal barrier function. Similarly, using the lactulose to mannitol differential absorption test, Huang et al.³¹ reported that sodium butyrate (1g/kg body mass) decreased the intestinal permeability of weaned piglets. The present study demonstrates that supplemental sodium butyrate improves growth performance, ameliorates weaning-associated intestinal injury and enhances intestinal barrier function.

Weaning-associated intestinal inflammation has negative effects on intestinal integrity and epithelial function in piglets.^4,32 It has been reported that mast cell activation and inflammatory mediator release play a major role in the intestinal barrier dysfunction during the post-weaning period.¹ Mast cells are considered to be an important cell type and play central roles in mediating intestinal inflammation.³³ Upon activation, preformed mediators stored in granules are released immediately, resulting in increase of intestinal permeability.^34,35 So far, only a few studies in vitro have reported that butyrate inhibits mast cell degranulation.^18,36 This study, for the first time, demonstrated that dietary supplementation with sodium butyrate decreased the percentage of degranulated mast cell in weaned pigs. In addition, sodium butyrate reduced the content of mast cell inflammatory mediators in mucosa of weaned pigs, including histamine, tryptase, TNF-α and IL-6. These results are supported by previous observations in murine mast cell line CPII clone 12, where butyrate inhibited both degranulation and TNF-α release.³⁶ However, in mouse bone marrow-derived mast cells, Zhang et al.¹⁹ found that butyrate suppresses FcɛRI-dependent cytokine release but had no effect on degranulation. The discrepancies might be related to the difference in cell models. The present study shows that dietary supplementation with sodium butyrate inhibited mast cell activation and release of its inflammatory mediators, thereby maintaining intestinal barrier function.

Several studies have shown that pro-inflammatory cytokines have negative effects on intestinal integrity and epithelial function.^27,37 Controlling the release of intestinal pro-inflammatory cytokines may have potential benefits in alleviating gut disorders.³⁸ Previous studies reported that butyrate inhibited the inflammatory cytokines mRNA levels in mast cell in vitro.^18,19 Therefore, we further investigated the effect of sodium butyrate on the relative mRNA expression of mast cell inflammatory mediators in the jejunal mucosa of weaned pigs. The results showed that dietary addition of sodium butyrate decreased the mRNA levels of mast cell-specific tryptase (MCT7), TNF-α and IL-6 in jejunal mucosa. It could be suggested that sodium butyrate improves intestinal integrity partially by inhibiting the mRNA expression of mast cell mediators in weaned pigs.

It has been reported that the MAPK signaling pathways regulate cytokine production in activated mast cells.^23,39 To our knowledge, only a few studies in cell models in vitro have investigated the influence of sodium butyrate on MAPK signaling pathways.^19,36 Diakos et al.³⁶ reported in murine mast cell line CPII clone 12 that butyrate treatment inhibited phosphorylation of JNK but not of p38 or ERK after dinitrophenyl-albumin challenge. In contrast to previous results, Zhang et al.¹⁹ reported that sodium butyrate simultaneously inhibited the three MAPK signaling pathways in murine bone marrow-derived mast cells after trinitrophenol BSA stimulation. However, there has been no study regarding the effects of sodium butyrate on MAPK signaling pathways in vivo. In the present study, for the first time, we found that supplemental sodium butyrate significantly decreased the relative protein levels for phosphorylated JNK but did not affect phosphorylated ERK and p38 of jejunum mucosa in weaned pigs. Taken together, it is likely that the role of sodium butyrate in improving intestinal barrier function of weaned pigs is related to inhibition of JNK signaling pathway. However, the exact role of this signaling pathway remains to be elucidated. In a follow-up study, the specific inhibitors of JNK signaling pathway may be used to investigate whether inhibition of JNK can affect the beneficial role of sodium butyrate on mast cell degranulation and the intestinal barrier of weaned piglets.

In summary, dietary supplementation with sodium butyrate ameliorated weaning-associated growth retardation and intestinal injury, and inhibited mast cell activation and production of inflammatory mediators. The JNK signaling pathway is involved in the beneficial role of sodium butyrate on mast cell degranulation and intestinal barrier of weaned piglets.

Footnotes

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This research was supported by National Key R & D Program (2016YFD0501210), Zhejiang Province Key R & D Project (2015C02022), Zhejiang Province Major Science and Technology Project (2015C03006), Special Fund for Agro-scientific Research in the Public Interest (201403047), Dabeinong Funds for Discipline Development and Talent Training in Zhejiang University.

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