Vitamin D has been introduced as one of the main regulators of spermatogenesis. Here, for the first time, we evaluated the expression of vitamin D receptor (VDR) and 1α-hydroxylase in all organs of male mice reproductive tract by immunohistochemistry and Western blotting. Epithelial cells of epididymis, seminal vesicle, coagulating gland, ductus deferens, preputial gland, and prostate were the prominent cell types that concomitantly expressed VDR and 1α-hydroxylase. Nearly all cell types in testis expressed both proteins. Interestingly, VDR intensity in epididymis epithelial cells was reduced toward cauda, in which only strong staining of stereocilia was observed. Although been positive in caput epididymis, sperms lost their VDR expression in cauda region. In all organs, sperms failed to express 1α-hydroxylase. Specific bands of the VDR and 1α-hydroxylase were determined in all tissues, except testis in which novel unprecedented isoforms of 1α-hydroxylase were observed. Our findings could provide further convincing evidence of pivotal role of this hormone in male reproductive biology.
FoxJGBartholdSWDavissonMTNewcomerCEQuimbyFWSmithAL. The Mouse in Biomedical Research, Normative Biology, Husbandry, and Models. 2nd ed. Burlington, MA: Elsevier, Inc; 2007.
2.
KwiecinskiGGPetrieGIDeLucaHF. Vitamin D is necessary for reproductive functions of the male rat. J Nutr. 1989;119(5):741–744.
3.
SchleicherGPrivetteTHStumpfWE. Distribution of soltriol [1,25(OH)2-vitamin D3] binding sites in male sex organs of the mouse: an autoradiographic study. J Histochem Cytochem. 1989;37(7):1083–1086.
4.
UhlandAMKwiecinskiGGDeLucaHF. Normalization of serum calcium restores fertility in vitamin D-deficient male rats. J Nutr. 1992;122(6):1338–1344.
5.
AudetILaforestJPMartineauGPMatteJJ. Effect of vitamin supplements on some aspects of performance, vitamin status, and semen quality in boars. J Anim Sci. 2004;82(2):626–633.
6.
HiraiTTsujimuraAUedaTEffect of 1,25-dihydroxyvitamin d on testicular morphology and gene expression in experimental cryptorchid mouse: testis specific cDNA microarray analysis and potential implication in male infertility. J Urol. 2009;181(3):1487–1492.
7.
JonesGStrugnellSADeLucaHF. Current understanding of the molecular actions of vitamin D. Physiol Rev. 1998;78(4):1193–1231.
8.
ChenTLHauschkaPVCabralesSFeldmanD. The effects of 1,25-dihydroxyvitamin D3 and dexamethasone on rat osteoblast-like primary cell cultures: receptor occupancy and functional expression patterns for three different bioresponses. Endocrinology. 1986;118(1):250–259.
9.
HalloranBPDeLucaHF. Appearance of the intestinal cytosolic receptor for 1,25-dihydroxyvitamin D3 during neonatal development in the rat. J Biol Chem. 1981;256(14):7338–7342.
10.
ZarnaniAHShahbaziMSalek-MoghaddamAVitamin D3 receptor is expressed in the endometrium of cycling mice throughout the estrous cycle. Fertil Steril. 2010;93(8):2738–2743.
11.
ShahbaziMJeddi-TehraniMZareieMExpression profiling of vitamin D receptor in placenta, decidua and ovary of pregnant mice. Placenta. 2011;32(9):657–664.
12.
TavakoliMJeddi-TehraniMSalek-MoghaddamAEffects of 1,25(OH)2 vitamin D3 on cytokine production by endometrial cells of women with recurrent spontaneous abortion. Fertil Steril. 2011;96(3):751–757.
13.
Perez-LopezFR. Vitamin D: the secosteroid hormone and human reproduction. Gynecol Endocrinol. 2007;23(1):13–24.
14.
EvansKNBulmerJNKilbyMDHewisonM. Vitamin D and placental-decidual function. J Soc Gynecol Investig. 2004;11(5):263–271.
15.
HalloranBPDeLucaHF. Effect of vitamin D deficiency on fertility and reproductive capacity in the female rat. J Nutr. 1980;110(8):1573–1580.
16.
KwiecinksiGGPetrieGI, DeLuca HF. 1,25-Dihydroxyvitamin D3 restores fertility of vitamin D-deficient female rats. Am J Physiol. 1989;256(4 pt 1):E483–E487.
17.
LeeDMTajarAPyeSRAssociation of hypogonadism with vitamin D status: the European Male Ageing Study. Eur J Endocrinol. 2012;166(1):77–85.
18.
YoshidaMKawanoNYoshidaK. Control of sperm motility and fertility: diverse factors and common mechanisms. Cell Mol Life Sci. 2008;65(21):3446–3457.
19.
Jeddi-TehraniMAbbasiNDokouhakiPIndoleamine 2,3-dioxygenase is expressed in the endometrium of cycling mice throughout the oestrous cycle. J Reprod Immunol. 2009;80(1-2):41–48.
20.
SonnenbergJLuineVNKreyLC, Christakos S. 1,25-Dihydroxyvitamin D3 treatment results in increased choline acetyltransferase activity in specific brain nuclei. Endocrinology. 1986;118(4):1433–1439.
21.
HardyMPGaoHBDongQStress hormone and male reproductive function. Cell Tissue Res. 2005;322(1):147–153.
22.
HodginsMB, Murad S. 1, 25-Dihydroxycholecalciferol stimulates conversion of androstenedione into oestrone by human skin fibroblasts in culture. J Endocrinol. 1986;110(1):R1–R4.
23.
KinutaKTanakaHMoriwakeTVitamin D is an important factor in estrogen biosynthesis of both female and male gonads. Endocrinology. 2000;141(4):1317–1324.
24.
JohnsonJAGrandeJPRochePCKumarR. Immunohistochemical detection and distribution of the 1,25-dihydroxyvitamin D3 receptor in rat reproductive tissues. Histochem Cell Biol. 1996;105(1):7–15.
25.
Blomberg JensenMNielsenJEJorgensenAVitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract. Hum Reprod. 2010;25(5):1303–1311.
26.
AudranMGrossMKumarR. The physiology of the vitamin D endocrine system. Semin Nephrol. 1986;6(1):4–20.
27.
JohnsonJAKumarR. Renal and intestinal calcium transport: roles of vitamin D and vitamin D-dependent calcium binding proteins. Semin Nephrol. 1994;14(2):119–128.
28.
JohnsonJAKumarR. Vitamin D and renal calcium transport. Curr Opin Nephrol Hypertens. 1994;3(4):424–429.
29.
Blomberg JensenMJorgensenANielsenJEExpression of the vitamin D metabolizing enzyme CYP24A1 at the annulus of human spermatozoa may serve as a novel marker of semen quality. Int J Androl. 2012;35(4):499–510.
30.
KumarRSchaeferJGrandeJPRochePC. Immunolocalization of calcitriol receptor, 24-hydroxylase cytochrome P-450, and calbindin D28k in human kidney. Am J Physiol. 1994;266(3 pt 2):F477–F485.
31.
MendozaFJPerez-MarinCCGarcia-MarinLLocalization, distribution, and function of the calcium-sensing receptor in sperm. J Androl. 2012;33(1):96–104.
32.
RenD.Calcium signaling in sperm: help from prostasomes. Sci Signal. 2011;4(173):pe27.
33.
QuillTASugdenSARossiKLHyperactivated sperm motility driven by CatSper2 is required for fertilization. Proc Natl Acad Sci U S A. 2003;100(25):14869–14874.
34.
Blomberg JensenMBjerrumPJJessenTEVitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa. Hum Reprod. 2011;26(6):1307–1317.
35.
KidroniGHar-NirRMenezelJVitamin D3 metabolites in rat epididymis: high 24,25-dihydroxy vitamin D3 levels in the cauda region. Biochem Biophys Res Commun. 1983;113(3):982–989.
36.
KalsotraACooperTA. Functional consequences of developmentally regulated alternative splicing. Nat Rev Genet. 2011;12(10):715–729.
