Is There a Link Between Expression Levels of Histone Deacetylase/Acetyltransferase in Mouse Sperm and Subsequent Blastocyst Development?

Abstract

Histone acetylation has been known to be significant in spermatogenesis. Histone acetylation is regulated by the act of histone deacetylases (HDACs) and histone acetyltransferases (HATs). We investigated the link between expression levels of HDACs and HATs in mouse sperm and subsequent blastocyst formation rate. In the univariate analysis, expression levels of HDAC1 and HAT were generally not associated with the blastocyst formation rate. When divided by the mature oocyte number category, a significant positive association was observed between the expression levels of HDAC1 and the blastocyst-forming rate in the highest (> 75th) percentile group (a group with ≥34 mature oocytes). In conclusion, expression of sperm HDAC1 could be considered as a possible predictor of embryo development in mice with high ovarian response.

Keywords

histone acetylation histone deacetylase sperm mouse blastocyst

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References

Campos

Reinberg

. Histones: annotating chromatin. Annu Rev Genet. 2009;43:559–599.

Turner

. Histone acetylation and an epigenetic code. Bioessays. 2000;22(9):836–845.

Davie

. Covalent modifications of histones: expression from chromatin templates. Curr Opin Genet Dev. 1998;8(2):173–178.

Meistrich

Trostle-Weige

Lin

Bhatnagar

Allis

. Highly acetylated H4 is associated with histone displacement in rat spermatids. Mol Reprod Dev. 1992;31(3):170–181.

Hazzouri

Pivot-Pajot

Faure

. Regulated hyperacetylation of core histones during mouse spermatogenesis: involvement of histone deacetylases. Eur J Cell Biol. 2000;79(12):950–960.

Kim

Jee

Lee

Suh

Kim

. Histone acetylation level and histone acetyltransferase/deacetylase activity in ejaculated sperm from normozoospermic men. Yonsei Med J. 2014;55(5):1333–1340.

Steger

Cavalcanti

Schuppe

. Prognostic markers for competent human spermatozoa: fertilizing capacity and contribution to the embryo. Int J Androl. 2011;34(6 pt 1):513–527.

Zhang

Xiao

Wang

. Effects of histone deacetylase inhibitors on the early development of bovine androgenetic embryos. Cell Reprogram. 2014;16(1):54–64.

Oliveira

Saraiva

de Souza

Tetzner

de Lima

Garcia

. Effects of histone hyperacetylation on the preimplantation development of male and female bovine embryos. Reprod Fertil Dev. 2010;22(6):1041–1048.

10.

Simon

Murphy

Shamsi

. Paternal influence of sperm DNA integrity on early embryonic development. Hum Reprod. 2014;29(11):2402–2412.

11.

McReynolds

Dzieciatkowska

Stevens

Hansen

Schoolcraft

Katz-Jaffe

. Toward the identification of a subset of unexplained infertility: a sperm proteomic approach. Fertil Steril. 2014;102(3):692–699.

12.

Mayer

Niveleau

Walter

Fundele

Haaf

. Demethylation of the zygotic paternal genome. Nature. 2000;403(6769):501–502.

13.

Lalancette

Miller

Krawetz

. Paternal contributions: new functional insights for spermatozoal RNA. J Cell Biochem. 2008;104(5):1570–1579.

14.

Jin

Huang

Pan

. Effect of sperm DNA fragmentation on IVF/ICSI clinical outcomes is diversified in women with different ovarian reserve. Abstract presented at 2014 Annual meeting of American Society of Reproductive Medicine.

15.

Kistler

Henriksen

Mali

Parvinen

. Sequential expression of nucleoproteins during rat spermiogenesis. Exp Cell Res. 1996;225(2):374–381.

16.

Sonnack

Failing

Bergmann

Steger

. Expression of hyperacetylated histone H4 during normal and impaired human spermatogenesis. Andrologia. 2002;34(6):384–390.

17.

Grozinger

Schreiber

. Deacetylase enzymes: biological functions and the use of small-molecule inhibitors. Chem Biol. 2002;9(1):3–16.

18.

Garrido

Meseguer

Alvarez

Simón

Pellicer

Remohí

. Relationship among standard semen parameters, glutathione peroxidase/glutathione reductase activity, and mRNA expression and reduced glutathione content in ejaculated spermatozoa from fertile and infertile men. Fertil Steril. 2004;82(suppl 3):1059–1066.