The leaves of Cleome arabica L. (Capparaceae), which contain a number of glucosylated and rhamnosylated flavonols, possess anti-inflammatory activity and are used for the treatment of abdominal and rheumatic pains. In this study, we examine the effects of C. arabica leaf extracts (CALE) treatment with naringinase on selected properties of polymorphonuclear leukocytes (PMNs). Chemotaxis of these cells was investigated in the presence of either CALE or CALE treated with naringinase. Results showed that CALE reduced the directed movement of PMNs induced by fMet-Leu-Phe in a dose-dependent manner. A previous treatment of CALE with naringinase enhanced this effect. We conclude that deglycosylation of flavonoids in CALE by naringinase improves the beneficial effect of this plant extract on PMNs. The ability of C. arabica leaf extract treated with naringinase to reduce chemotaxis in human neutrophils may be an important therapeutic factor in the treatment of chronic diseases.
MiddletonEJR, KandaswamiC, TheoharidesTC. (2000) The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacological Reviews, 52, 673–751.
2.
IsmailIS, ItoH, SelloumL, YoshidaT. (2005) Flavonoids from Cleome arabica leaves and twigs. Natural Medicine, 59, 53.
3.
BouricheH, SelloumL, TigrineC, BoudoukhaC. (2003) Effect of Cleome arabica leaf extract on rat paw oedema and human neutrophil migration. Pharmaceutical Biology, 41, 10–15.
4.
BouricheH, MilesEA, SelloumL, CalderPC. (2005) Effect of Cleome arabica leaf extract, rutin and quercetin on soybean lipoxygenase activity and on generation of inflammatory eicosanoids by human neutrophils. Prostaglandines, Leukotrienes and Essential Fatty Acids, 72, 195–201.
5.
SchillerJ, BenardS, ReichlS, ArnholdJ, ArnoldK. (2000) Cartilage degradation by stimulated human neutrophils: reactive oxygen species decrease markedly the activity of proteolytic enzymes. Chemistry & Biology, 7, 557–568.
6.
HiiCS, MarinLA., HallidayD, RobertonDM, MurrayAW, FerranteA. (2001) Regulation of human neutrophil-mediated cartilage proteoglycan degradation by phosphatidylinositol-3-kinase. Immunology, 102, 59–66.
7.
ChenYC, ShenS C, LinHY. (2003) Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids. Biochemical Pharmacology, 66, 1139–1150.
8.
SelloumL, DjeliliH, SebihiL, ArnholdJ. (2004) Scavenger effect of flavonols on HOCl-induced luminol chemiluminescence. Luminescence, 19, 199–204.
9.
ShenSC, LeeWR, LinHY, HuangHC, KoCH, YangLL, ChenYC. (2002) In vitro and in vivo inhibitory activities of rutin, wogonin, and quercetin on lipopolysaccharide-induced nitric oxide and prostaglandin E2 production. European Journal of Pharmacology, 446, 187–194.
10.
LinHY, ShenSC, ChenYC. (2005) Anti-inflammatory effect of heme oxygenase 1: glycosylation and nitric oxide inhibition in macrophages. Journal of Cell Physiology, 202, 579–590.
11.
MichonF, PozsgayV, BrissonJR, JenningsHJ. (1989) Substrate specificity of naringinase, an alpha-L-rhamnosidase from Penicillium decumbens. Carbohydrate Research, 194, 321–324.
12.
VlahosCJ, MatterWF, HuiKY, BrownRF. (1994) A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY 294002). The Journal of Biological Chemistry, 269, 5241–5248.
13.
Gamet-PayrastreL, ManentiS, GratacapMP, TulliezJ, ChapH, PayrastreB. (1999). Flavonoids and the inhibition of PKC and PI 3-kinase. General Pharmacology, 32, 279–286.
14.
PayrastreB, MissyK, GiuriatoS, BodinS, PlantavideM, GratacapMP. (2001) Phosphoinositides: key players in cell signaling, in time and space. Cell Signaling, 13, 377–387.
15.
ZielińskaM, KostrzewaA, IgnatowiczE, BudzianowskiJ. (2001) The flavonoids, quercetin and isorhamnetin 3-O-acylglucosides diminish neutrophil oxidative metabolism and lipid peroxidation. Acta Biochimica Polonica, 48, 183–189.
16.
TriboloS, LodiF, ConnorC, SuriS, WilsonVG, TaylorMA, NeedsPW, KroonPA, HughesDA. (2008) Comparative effects of quercetin and its predominant human metabolites on adhesion molecule expression in activated human vascular endothelial cells. Atherosclerosis, 197, 50–56.
17.
PuriM, BanerjeeUC. (2000) Production, purification, and characterization of the debittering enzyme naringinase. Biotechnology Advances, 18, 207–217.
18.
OvaskainenML, TörrönenR, KoponenJM, SinkkoH, HellströmJ, ReinivuoH, MattilaP. (2008) Dietary intake and major food sources of polyphenols in Finnish adults. The Journal of Nutrition, 138, 562–566.
19.
KomuraS, NagataN, OhishiN, YagiK. (1995). Protective effect of catecholestrogen on lipid hydroperoxide-induced injury to cultured bovine aortic endothelial cells and on lipid peroxidation of low-density lipoprotein. Journal of Clinical Biochemistry and Nutrition, 19, 71–77.
20.
FalkW, GoodwinRHJr, LeonardEJ. (1980) A 48-well microchemotaxis assembly for rapid and accurate measurement of leukocyte migration. Journal of Immunological Methods, 33, 239–247
