Antioxidant and Antibacterial Constituents from Two Sumatran Ferns,Trichomanes javanicum and Oleandra pistillaris

Mangiferin (1), vitexin (2) and 4-O-β-D-glucopyranosylcaffeic acid (3), by means of classical extraction, fractionation and chromatographic separation, have been isolated for the first time from aerial parts (stems and leaves) of Trichomanes javanicum Blume (Hymenophyllaceae) as well as 3,4-dihydroxybenzoic acid (4) and gallic acid (5) from the rhizome of Oleandra pistillaris (Sw.) C. Chr. (Oleandraceae). These compounds were characterized by spectroscopic methods and then compared to those of data reported in the literature. Compound (1) and (4) showed significant antioxidant activity (IC₅₀ value was 3.89 and 3.83 μM respectively) twice stronger than ascorbic acid as standard (IC₅₀ value 7.86 μM). Compound (4) and (5) significantly inhibited the growth of Salmonella thypimurium and S. thypi while compounds (1), (2) and (3) showed weak inhibition toward Staphylococcus aureus, Bacillus subtilis and Vibrio cholerae. All compounds were not active against Enterococcus faecalis and Streptococcus mutans.