Fatty Acid Esters and Acyclic Monoterpenoid from Justicia insularis Leaf Fractions Attenuated Malaria Pathogenesis Through Docking with Plasmodium falciparum Serine Hydroxymethyl Transferase and Plasmodium falciparum Erythrocyte Membrane Protein 1 Proteins

Abstract

Background

Justicia insularis (Acanthaceae) T. Anderson is ethnopharmacologically used in Nigeria for the treatment of diseases including malaria. Therefore, this study was designed to investigate in vivo antiplasmodial effect of J. insularis leaf in Plasmodium berghei-infected mice, characterize its constituents, and carryout in silico studies of its compounds.

Methods

Standard protocols were followed in the processing of the plant leaves, extraction, fractionation, isolation, and characterization, evaluation of in vivo antiplasmodial assay, retrieval of Plasmodium falciparum serine hydroxymethyl transferase (PfSHMT) and Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP-1) proteins, absorption, distribution, metabolism, excretion, and toxicity (ADMET), and docking studies. Gas chromatography-mass spectrometry (GC-MS) was employed to isolate and characterize the compounds; SWISSADME and ADMET lab 2 enhanced the evaluation of pharmacokinetic properties, PyRx for docking analysis; Biovia discovery studio for 2D visualization, and PyMol software for 3D visualization of the ligand-protein interactions.

Results

The dichloromethane (61.59%) and ethyl acetate (73.15%) fractions had the best therapeutic indices and compared favorably with chloroquine (81.58%) in the curative antiplasmodial assay. The GC-MS analysis revealed 20 already reported antiplasmodial compounds with hexanoic acid 1,1-dimethylethyl ester, octadecanoic acid docosyl ester, and trans-β-ocimene as the lead compounds based on their binding affinities, permeation of the blood-brain-barrier, non-inhibition of metabolizing enzymes, ease of excretion, non-carcinogenicity, as well as non-violation of Lipinski's criteria.

Conclusion

Octadecanoic acid docosyl ester and hexanoic acid 1,1-dimethylethyl ester bonded the tetrahydrofolate-binding sites of PfSHMT, caused inhibition of DNA synthesis, and apoptosis, whereas trans-β-ocimene inhibited PfEMP-1, reversed the attachment of parasitized red blood cells to micro-vascular endothelium as their mechanism of action for parasitemia clearance. Moreover, these lead compounds reported for the first time in the dichloromethane and EtoAc fractions of this plant are responsible for the remarkable antiplasmodial activity observed in this study.

Keywords

Malaria docking PfSHMT PfEMP-1 and PfHT-1 proteins

Introduction

Malaria is a potentially fatal disease caused by Plasmodium falciparum abbreviated P. falciparum.¹ In 2019, the estimated global malaria prevalence was 229 million cases with mortality rate of 409,000 persons, predominantly, young children in sub-Saharan Africa.² Nigeria had the highest number of global malaria prevalence (25% of global malaria prevalence) and accounted for the highest number of mortality (24% of global malaria mortality).^3,4 More so, a study revealed resistant strains of P. falciparum to common antimalarial chemotherapies,⁵ necessitating the development of novel antimalarial medications. So, diverse protein targets and pathways have been studied for the unraveling of novel medicines; however, others are still under clinical trials.⁶ Therefore, the screening of many chemical libraries against protein targets is necessary in the investigation of antimalarial targets from novel pathways unrelated to drug resistance.⁷

Moreover, P. falciparum known to enhance serious malaria pathogenesis⁸ could invade red blood cell (RBC) and liver cells of people; however, it is believed that P. falciparum erythrocyte membrane protein 1 (PfEMP-1) binds to the endothelial cell receptors CD-36, thrombospondin, and intercellular adhesion molecule 1, and exists a receptor related to malaria that mediates attachment of parasitized red blood cells (pRBCs) to micro-vascular endothelium. The specific binding point on PfEMP-1 and corresponding receptors on the host cells could potentially serve as targets for creating substances aimed at reversing the binding of pRBCs, thereby, stopping the blockage of micro-vessels and offering a promising approach for treating malaria.^8,9

Also, P. falciparum serine hydroxymethyl transferase (PfSHMT), a pyridoxal-5′-phosphate-dependent enzyme is known to catalyze the reversible conversion of serine and tetrahydrofolate (THF) through the α-elimination and replacement mechanism in order to yield glycine and 5,10-methylenetetrahydrofolate (CH₂-THF).¹⁰ It is a part of the deoxythymidylate (dTMP) synthesis cycle where dTMP (a nucleotide precursor for DNA synthesis) is biosynthesized, and the oxidized folate compounds thus generated are recycled by a synchronizing cooperation of PfSHMT and the other two enzymes in the cycle: dihydrofolate reductase (DHFR) and thymidylate synthase (TS).¹⁰ At present, drugs targeting TS and DHFR in malaria parasites and cancer cells have been compromised due to target mutation¹¹; therefore, alternative drugs that have new enzyme targets are urgently needed. PfSHMT has gained increasing attention as a new potential antimalarial and anticancer drug target.¹¹ Although several inhibitors of cytosolic PfSHMT have been reported,¹² attempts to search for new effective compounds have continued to reduce drug resistance challenges. The effective compounds are proposed to compete with THF and l-serine substrates of PfSHMT in competitive and non-competitive inhibition in order to bind the THF-binding sites of PfSHMT; thus, paving the way to develop new antifolate drugs.^12,13

It is worthy of note that the most effective antimalarial medications have been derived from natural sources throughout the history of malaria chemotherapy;⁷ conversely, Asanga et al¹⁴ corroborated that the tropical forests in Nigeria are vested with many medicinal plants; so, robust researches and implementation of research findings, the isolation and characterization of bioactive compounds in these plants, as well as the integration of orthodox and traditional medicine could drastically boost health care delivery in Nigeria. One of these medicinal plants with reported ethnopharmacological relevance as well as its natural products with reported pharmacological activities is Justicia insularis abbreviated J. insularis.

J. insularis T. Anderson (Acanthaceae family) is a vegetable used for both nutritional and medicinal purposes, such as digestive, weaning agent, malaria remedy, and laxative.¹⁵ The leaf extract of the plant has been reported to enhance ovarian folliculogenesis and fertility in female rats,¹⁶ antioxidant,¹⁷ and antianemic¹⁸ activities. Compounds such as clerodane diterpenoids (16(α/β)-hydroxy-cleroda-3,13 (14)Z-dien-15,16-olide and 2,16-oxo-cleroda-3,13(14)E-dien-15-oic acid) have been isolated and characterized from the plant's leaf extract.¹⁹

In addition, despite the management of malaria with traditional medicine and chemotherapies, the validation of the mode of action of the lead compounds is a serious concern. So, the discovery of therapeutics has been a serious concern on how ligands are docked with their receptors⁸; therefore, the initial phase of drug discovery and development involves lead identification. During this process, chemical compounds are challenged to interact with their protein targets to alter their functions. In order to be effective against some target proteins like PfSHMT and PfEMP-1; lead compounds should ideally exhibit some degree of precision and potency. Hence, this study was geared towards the validation of the antiplasmodial potentials of J. Insularis leaves, isolation and characterization of its bioactive compounds using gas chromatography-mass spectrometry (GC-MS), as well as the in silico prediction of its ligands against PfSHMT and PfEMP-1 proteins.

Results and Discussion

Results

The Antiplasmodial Activities of the Extract and Fractions of J. insularis Leaves

The leaf extract and its fractions exerted dose-dependent reductions in parasitaemia of the treated mice in the suppressive antiplasmodial assay. From Table 1, the parasitemia reduction was only statistically significant (P < 0.01) at the highest dose when compared to the control; however, the chemosuppression of the parasites and mean survival time (MST) for EtoAc fraction (64.21% and 27.00 days) was higher than dichloromethane (DCM) fraction (51.04% and 22.33 days) but favorably comparable to chloroquine (72.65% and 29.50 days). Also, from Table 2, the parasitemia reductions in the repository antiplasmodial assay were statistically significant (P < 0.01-0.001) relative to the control at higher doses of the extract (300 and 450 mg/kg) body weight of mice. Here, the chemosuppression of the parasites and MST for pyrimethamine (81.82% and 29.00 days) was better than the extract (450 mg/kg) (38.48% and 19.00 days) and EtoAc fraction (26.56% and 18.33 days), respectively. Moreover, Table 3 revealed the result for the schizonticidal antiplasmodial assay evidenced with progressive dose-dependent reduction of parasitaemia in all the extract/fraction-treated groups relative to control as well as statistical significance (P < 0.001) relative to the negative control. The chemosuppression of the parasites and MST for EtoAc fraction (73.15% and 28.66 days) was higher than DCM fraction (61.59% and 25.00 days) and favorably comparable to chloroquine (81.58% and 29.83 days).

Table 1.

Suppressive Antiplasmodial Activities of the Extract and Fractions of J. insularis Leaf (n = 5).

Treatment groups	Dose (mg/kg)	Parasitaemia	Chemosuppression (%)	MST (days)
Negative control	–	31.66 ± 1.87	–	7.83 ± 0.30
Extract	150	22.0 ± 1.29	30.51	10.10 ± 0.70*
	300	21.5 ± 1.08	32.09	12.00 ± 0.33**
	450	18.33 ± 1.54**	42.10	19.03 ± 1.32**
n-hex fraction	300	22.83 ± 0.54	27.89	9.00 ± 0.42
DCM fraction	300	15.50 ± 0.61**	51.04	22.33 ± 1.11***
EtoAc fraction	300	11.33 ± 1.49**	64.21	27.00 ± 1.39***
n-BuOH fraction	300	20.33 ± 5.66*	35.78	14.0 ± 0.57*
Chloroquine	5	8.66 ± 0.84***	72.65	29.50 ± 0.34***

Values are expressed as mean ± SEM. Significance relative to control. *P < 0.05; **P < 0.01; ***P < 0.001.

DCM, dichloromethane; MST, mean survival time; SEM, standard error of mean.

Table 2.

Prophylactic Antiplasmodial Activities of the Extract and Fractions of J. insularis Leaf (n = 5).

Treatment groups	Dose (mg/kg)	Parasitaemia	Chemosuppression (%)	MST (days)
Negative control	–	23.83 ± 1.42	–	7.83 ± 0.30
Extract	150	20.83 ± 1.22	12.59	10.83 ± 0.60
	300	17.66 ± 1.05**	25.89	16.33 ± 0.60*
	450	14.66 ± 0.95***	38.48	19.00 ± 0.51*
n-hex fraction	300	21.16 ± 0.87	11.20	8.83 ± 0.30
DCM fraction	300	19.0 ± 1.29	20.26	15.00 ± 0.42
EtoAc fraction	300	17.5 ± 1.02*	26.56	18.33 ± 0.42***
n-BuOH fraction	300	21.0 ± 1.23	11.87	9.03 ± 0.42
Pyrimethamine	1.2	4.33 ± 0.66***	81.82	29.00 ± 1.22***

Values are expressed as mean ± SEM. Significant relative to control. *P < 0.05; **P < 0.01; ***P < 0.001.

DCM, dichloromethane; MST, mean survival time; SEM, standard error of mean.

Table 3.

Schizonticidal Antiplasmodial Activities of the Extract and Fractions of J. insularis Leaf (n = 5).

Treatment groups	Dose (mg/kg)	MST (Days)	Parasitaemia (%)	Chemosuppression (%)
Negative control	-	7.66 ± 0.33	31.66 ± 1.87	–
Extract	150	18.00 ± 0.57	21.0 ± 0.57***	33.67
	300	21.33 ± 0.49***	17.83 ± 1.57***	43.68
	450	22.33 ± 0.88c	14.50 ± 1.60***	54.20
n-hex fraction	300	19.16 ± 0.47**	19.50 ± 1.17***	38.40
DCM fraction	300	25.00 ± 0.57***	12.16 ± 1.30***	61.59
EtoAc fraction	300	28.66 ± 0.80***	8.50 ± 0.99***	73.15
n-BuOH fraction	300	17.33 ± 0.42*	22.33 ± 1.20***	29.46
Chloroquine	5	29.83 ± 0.16***	2.83 ± 0.99***	81.58

Values are expressed as mean ± SEM. Significant relative to control. *P < 0.05; **P < 0.01; ***P < 0.001.

DCM, dichloromethane, MST, mean survival time; SEM, standard error of mean.

The Effect of the Extract/Fractions of J. insularis Leaves on the Rectal Temperatures of Infected Mice

The administration of the extract and fractions of J. insularis leaves as well as chloroquine to P. berghei-infected mice in curative antiplasmodial test did not cause any significant difference (P > 0.05) in the rectal temperature (Table 4) of the treated mice when compared with the control.

Table 4.

Effect of the Extract and Fractions of J. insularis Leaves (n = 5) on Rectal Temperature of Mice Infected with P. Berghei During Established Infection.

Treatment	Dose (mg/kg)	Rectal temperature (˚C)
Treatment	Dose (mg/kg)	D₀	D₃	D₅	D₇
Negative control	–	34.86 ± 0.46	35.22 ± 0.02	35.22 ± 0.02	35.75 ± 0.06
Extract	150	35.47 ± 0.07	35.52 ± 0.04	35.53 ± 0.02	35.42 ± 0.07
	300	35.47 ± 0.07	35.43 ± 0.02	35.33 ± 0.02	35.30 ± 0.04
	450	35.50 ± 0.04	35.32 ± 0.02	35.32 ± 0.02	35.23 ± 0.02
n-hex fraction	300	35.47 ± 0.04	35.40 ± 0.02	35.30 ± 0.02	35.50 ± 0.04
DCM fraction	300	35.52 ± 0.04	35.30 ± 0.06	35.40 ± 0.04	35.33 ± 0.06
EtoAc fraction	300	35.52 ± 0.02	35.45 ± 0.04	35.33 ± 0.06	35.30 ± 0.06
n-BuOH fraction	300	35.00 ± 0.20	35.30 ± 0.10	35.30 ± 0.10	35.31 ± 0.04
Chloroquine	5	35.18 ± 0.27	35.37 ± 0.07	35.37 ± 0.07	35.22 ± 0.08

Values are expressed as mean ± SEM.

DCM, dichloromethane; SEM, standard error of mean.

GC-MS Analysis

GC-MS analysis (Table 5) of DCM fraction revealed some pharmacologically active compounds such as glyceraldehyde; hexanoic acid; 1,1-dimethylethyl ester; hexanoic acid, butyl ester; hexanoic acid, 2,4-dimethyl-, methyl ester; E-2-tetradecen-1-ol, oxirane, tetradecyl-; trans-β-ocimene; α-pinene among others. Also, the GC-MS analysis (Table 6) of EtoAc fraction indicated that it contains unsaturated fatty acids such as hexanoic acid; pentanoic acid, 3-methyl-; hexanoic acid, 1,1-dimethylethyl ester; hexadec-9-enoic acid; 7-tert-butyldimethylsilyloxy-, methyl ester; heneicosanoic acid, methyl ester; octa-2,4,6-triene; 1,3,6-heptatriene, 5-methyl-, (E)-; phytol, acetate; octadecanoic acid, 2-hydroxy-1,3-propanediyl ester; octadecanoic acid, docosyl ester; and others.

Table 5.

GCMS Analysis of DCM Fraction of J. insularis Leaf.

S/No	Retention time (RT)	Names of compounds	Chemical formula	Molecular masses
1.	5.129	Glyceraldehyde	C₃H₆O₃	90.031
2.	5.874	Hexanoic acid, 1,1-dimethylethyl ester	C₁₀H₂₀O₂	172.146
3.	5.944	Hexanoic acid, butyl ester	C₁₀H₂₀O₂	172.146
4.	7.137	Hexanoic acid, 2,4-dimethyl-, methyl ester, (2DL,4L)-	C₉H₁₈O₂	158.130
5.	11.916	E-2-Tetradecen-1-ol	C₁₄H₂₈O;	212.214
6.	12.055	Oxirane, tetradecyl-	C₁₆H₃₂O	240.245
7.	15.239	trans-β-Ocimene	C₁₀H₁₆	136.125
8.	15.949	α-Pinene	C₁₀H₁₆	136.125
9.	18.044	Tetracosan-1-ol trimethylsilyl ether	C₂₇H₅₈OSi	426.425
10.	24.440	Tetracosane, 9-octyl-	C₃₂H₆₆	450.516

DCM, dichloromethane.

Table 6.

GCMS Analysis of EtoAc Fraction of J. insularis Leaf.

S/No	Retention time (RT)	Names of compounds	Chemical formula	Molecular masses
1.	6.453	Hexanoic acid	C₆H₁₂O₂	116.083
2.	6.545	Pentanoic acid, 3-methyl-	C₆H₁₂O₂	116.083
3.	6.614	Hexanoic acid, 1,1-dimethylethyl ester	C₁₀H₂₀O₂	172.146
4.	6.825	Hexadec-9-enoic acid, 7-tert-butyldimethylsilyloxy-, methyl ester	C₂₃H₄₆O₃Si	398.321
5.	6.888	Heneicosanoic acid, methyl ester	C₂₂H₄₄O₂	340.334
6.	6.934	Octa-2,4,6-triene	C8H₁₂	108.093
7.	6.986	1,3,6-Heptatriene, 5-methyl-, (E)-	C₈H₁₂	108.093
8.	7.197	Phytol, acetate	C₂₂H₄₂O₂	338.318
9.	7.392	Octadecanoic acid, 2-hydroxy-1,3-propanediyl ester	C₃₉H₇₆O₅	624.569
10.	7.666	Octadecanoic acid, docosyl ester	C₄₀H₈₀O₂	592.615

Molecular Docking Analysis

The result (Table 7, Figures 1 and 2) of the docking analysis revealed the interactions of 1BJ4 with selected ligands (A1, A2, A3, A4, A5, and A6) and standard drug (CQ). Ligand A3 binding energy (−6.1 kcal/mol) was lower than that of CQ (−4.8 kcal/mol); however, that of ligands A1 (−4.7 kcal/mol) and A5 (−4.3 kcal/mol) were higher but compared favorably with the standard drug. Further analysis on the binding pocket of the protein revealed that ligand A3 formed an alkyl, pi-alkyl, and 3 hydrogen bonds with the amino acid residues at the active site of the protein. Also, the functional groups in ligand A1 were able to form 2 hydrogen bonds via Thr163 and Phe125 as well as pi-sigma bond with Ala162 at the active site of IBJ4. Consequently, ligand A5 generated both alkyl and pi-alkyl interactions with Ala162, Leu149, and Phe125 as the amino acid residues at the active site of IBJ4 at various bond distances whereas CQ interaction with IBJ4 revealed that Thr163 formed a conventional hydrogen bond while both Leu149 and Ala162 formed alkyl and pi-alkyl interactions with the functional groups in chloroquine, respectively.

Figure 1.

2D and 3D visualization of protein-ligand interaction of 1BJ4 with CQ, A1, A2, and A3.

Figure 2.

2D and 3D visualization of protein-ligand interaction of 1BJ4 with A4, A5, and A6.

Table 7.

Molecular Docking Analysis of Selected Compounds from the Fraction of J. insularis Against 1BJ4 and 3C64 Proteins.

Interactions	Binding affinities (kcal/mol)	No. of conventional hydrogen bonds
1BJ4 +CQ	−4.8	1
1BJ4 + A1	−4.7	2
1BJ4+ A2	−3.8	Nil
1BJ4 + A3	−6.1	3
1BJ4 + A4	−4.3	Nil
1BJ4 + A5	−4.4	Nil
1BJ4 + A6	−4.3	2
3C64 + CQ	−5.4	Nil
3C64 + A1	−4.4	1
3C64 + A2	−4.5	Nil
3C64 + A3	−4.5	Nil
3C64 + A4	−4.6	Nil
3C64 + A5	−4.8	Nil
3C64 + A6	−3.1	1

Keys: CQ = Chloroquine; A1= Hexanoic acid, 1,1-dimethylethyl ester; A2= Octa-2,4,6-triene; A3= Octadecanoic acid, docosyl ester; A4= Phytol, acetate; A5= Trans-β-Ocimene; A6= Glyceraldehyde

According to the results (Table 7, Figures 3 and 4) of the docking analysis, the interactions of 3C64 with chloroquine (CQ) and selected ligands (A1, A2, A3, A4, A5, and A6) occurred. The binding energy of CQ (−5.4 kcal/mol) was lower than those of the selected ligands; however, the binding energies of ligands A5 (−4.8 kcal/mol), A4 (−4.6 kcal/mol), A3 (−4.5 kcal/mol), and A2 (−4.5 kcal/mol) compared favorably with that of CQ. Further analysis of these ligands’ interactions at the active site of 3C64 revealed that CQ formed some carbon-hydrogen bonds via Asp21 and Lys24; Tyr55 and Ile20 revealed alkyl and pi-alkyl interactions with the functional groups in CQ, whereas Trp25 formed a pi-pi stacked interaction with the aromatic ring in CQ. Also, ligand A5 through Trp25 formed a pi-sigma bond with the protein whereas the presence of Lys24 at the protein's active site enhanced the formation of alkyl and pi-alkyl interactions with the functional groups in CQ. The interaction of 3C64 with ligands A2, A3, and A4 via their various amino acid residues at the protein's active site revealed majorly alkyl and pi-alkyl interactions with these ligands.

Figure 3.

2D and 3D visualization of protein-ligand interaction of 3C64 with CQ, A1, A2, and A3.

Figure 4.

2D and 3D visualization of protein-ligand interaction of 3C64 with A4, A5, and A6.

ADMET Studies on Selected Ligands and Chloroquine

The absorption, distribution, metabolism, excretion, and toxicity (ADMET) results (Table 8) revealed that the Caco-2 permeability values for ligands A1, A2, A4, A5, and A6 were −4.162, −4.726, −4.568, −4.557 and −5.123, respectively. More so, the gastrointestinal tract (GI) absorption indices for ligands A1 and A3 were high whereas ligands A1 and A2 showed maximal plasma protein binding (PPB) in percentages as 87.559% and 10.061%. Also, ligands A1, A3, and A5 revealed they could cross the blood-brain-barrier (BBB); however, of all the ligands, it was only A3 and A4 that showed they could inhibit CYP2C9. Moreover, ligands A1, A2, A4, and A5 revealed excellent clearance levels as 11.519, 9.583, 5.081, and 7.761, respectively. Although ligands A1 and A2 revealed they could induce minimal carcinogenicity, ligands A4, A5, and A6 are non-carcinogens. In addition, all the ligands with exception of ligands A3 obeyed Lipinski rule of five with at most one violation.

Table 8.

ADMET Properties of Selected Bioactive Compound.

Compounds	A1	A2	A3	A4	A5	A6
Physiochemical properties
Formula	C₁₀H₂₀O₂	C₈H₁₂	C₄₀O₂	C₂₂H₄₂O₂	C₁₀H₁₆	C₃H₆O₃
Molecular weight (g/mol)	172.26	108.18	512.43	338.57	136.23	90.08
Num. heavy atoms	12	8	42	24	10	6
TPSA (Å²)	9.23	0.00	9.23	9.23	0.00	40.46
Absorption
Caco-2 Permeability	-4.162	-4.726	-	-4.568	-4.557	-5.123
MDCK Permeability	2.1e-05	0.0002	-	1.2e-05	2.8e-05	0.0051
GI absorption	High	Low	High	Low	Low	Low
Pgp-substrate	No	No	Yes	Yes	No	No
Distribution
PPB	87.559%	96.450%	-	98.161%	97.488%	10.061%
VD	1.406	2.876	-	1.619	2.830	0.784
BBB Permeant	Yes	No	Yes	No	Yes	No
Metabolism
CYP1A2 inhibitor	No	No	No	No	No	No
CYP2C19 inhibitor	No	No	No	No	No	No
CYP2C9 inhibitor	No	No	Yes	Yes	No	No
CYP2D6 inhibitor	No	No	No	No	No	No
CYP3A4 inhibitor	No	No	No	No	No	Yes
Excretion
CL	11.519	9.583	-	5.081	7.761	3.223
T_1/2	0.760	0.547	-	0.063	0.391	0.796
Toxicity
Carcinogencity	+	+	Nil	–	–	–
Drug likeness
Lipinski	Yes; 0 violation	Yes; 1 violation: MLOGP > 4.15	No; 2 violations: MW > 500, MLOGP > 4.15	Yes; 1 violation: MLOGP > 4.15	Yes; 1 violation: MLOGP > 4.15	Yes; 0 violation
Ghose	Yes	No; 2 violations: MW < 160, MR < 40	No; 2 violations: MW > 480, MR > 130	No; 1 violation: WLOGP > 5.6	No; 1 violation: MW < 160	No; 4 violations: MW < 160, WLOGP < -0.4, MR < 40, #atoms < 20
Bioavailability score	0.55	0.55	0.17	0.55	0.55	0.55

GI, gastrointestinal tract; BBB, blood-brain-barrier; PPB, plasma protein binding; ADMET, absorption, distribution, metabolism, excretion, and toxicity.

Discussion

Malaria pathogenesis normally reveals hyperparasitaemia, hyperthermia, and hyperpyrexia²⁰; however, these symptoms if not averted with chemotherapies and medicinal plants could trigger deleterious complications with possibilities of mortality; so, J. insularis leaves are ethnopharmacologically used for the purpose of attenuating the above symptoms in patients. Therefore, this study was designed to validate the antimalarial potentials of this plant in order to provide scientific basis for its usage as an antimalarial plant. The study revealed that the extract and fractions significantly reduced parasitaemia in dose-dependent manner in all the antiplasmodial assay models with EtoAc and DCM fractions exhibiting the best chemosuppressive and schizonticidal activities; this result was in tandem with previous reports^8,21 on other medicinal plants having antiplasmodial activities and acting through the suppression of parasite growth as well as suicidal attack on its schizonts. It is worthy of note that the antiplasmodial activities of the following compounds have been reported hexadecanoic acid methyl ester; 9,12-octadecadienoic acid methyl ester (linoleic acid); 9,12,15-octadecatrienoic acid methyl ester (linolenic acid); and 9-octadecenoic acid^20,22,23; phytol;^24,25 and monoterpenes such as limonene and others (trans-β-ocimene and α-pinene)²⁶; therefore, these compounds profiled from GCMS analysis could have contributed to the observed antiplasmodial activity of J. insularis leaves through a particular mechanism of action. Also, the plant's ability to prolong the MST of the mice suggested their abilities in parasitaemia clearance, thereby, aiding the mitigation of malaria pathogenesis and its associated symptoms in mice. This result was consistent with the report¹⁴ on prolonged MST for mice treated with Nauclea latifolia roots.

Also, fever is one of the cardinal symptoms of malaria; however, P. berghei infection in mice is reported to be associated with hypothermia rather than pyrexia.²⁷ The result on rectal temperature of the infected mice in this study revealed insignificant difference between the mean temperature values of both the treated and negative control mice, suggesting that they were hypothermic. This hypothermia may have resulted from the various physiological effect of P. berghei in mice, leading to body heat loss, and death of mice.²⁸ More so, carbohydrate, lipid, and protein metabolisms in mice are negatively affected by P. berghei invasion²⁹; therefore, the decreased metabolic rate in these mice correlated their decreased body temperature³⁰; hence, the resultant hypothermia.

Moreover, molecular docking as a computational tool is designed to accentuate the best binding orientation that compounds interact with their target proteins; it reveals the biological efficacy as well as the binding affinities of compounds, thereby, enhancing the prediction of their potential therapeutic effect.³¹ The lowest binding affinities observed for octadecanoic acid docosyl ester and hexanoic acid, 1,1-dimethylethyl ester interaction with PfSHMT as well as trans-β-ocimene, phytol acetate, and octadecanoic acid docosyl ester interaction with PfEMP-1 that were better than chloroquine proved that these compounds’ antiplasmodial activities were through the inhibition of PfSHMT that led to the mitigation of DNA synthesis in the parasites as well as the inhibition of PfEMP-1, a receptor that mediates the attachment of pRBCs to micro-vascular endothelium, thereby, resulting in the parasitemia clearance. It is worthy of note that some researchers⁸ posited that lead compounds had lower docking scores; therefore, the lower score function during docking simulations is an advantage for the prediction of both strength and type of signals produced during the structure-based design of drugs. Conversely, the lower binding affinity in these compounds could be attributed to the presence of carbonyl group (C = O) that participated in the hydrogen bonding, Vander Waal forces, and electrostatic interactions, thus, facilitating the compounds interaction with these proteins. Consequently, the interaction of trans-β-ocimene (a monoterpenoid) with PfEMP-1 revealed a pi-sigma bond; however, Li et al³² reported that pi-sigma interaction contributed to specificity and stability of receptor-ligand interaction as well as influenced their binding affinities during docking simulations. Therefore, hexanoic acid 1,1-dimethylethyl ester and octadecanoic acid docosyl ester with the least binding energies had the best interaction and antiplasmodial activities against PfSHMT, implying that they competed with THF and l-serine substrates of PfSHMT in competitive and non-competitive inhibition in order to bind the THF-binding sites of PfSHMT, hence, paving the way for the development of new antifolate drugs for malaria treatment. Furthermore, trans-β-ocimene, phytol acetate, octadecanoic acid docosyl ester, and octa-2,4,6-triene revealed the lowest binding energies and similar antiplasmodial activity through the inhibition of PfEMP-1, being a malarial receptor that mediates the adherence of pRBCs to micro-vascular endothelium, therefore resulting in the reversal of pRBC adherence and consequent occlusion of micro-vessels leading to the death of the parasites.⁸

The evaluation of the ADMET properties of the ligands as part of in silico studies provided understanding of the compounds’ pharmacokinetics and physicochemical properties in the human system. The Caco-2 cells are often used by researchers to imitate the human intestinal wall because they act similarly as the human intestine.³² Here, the Caco-2 permeability values for all the compounds were within acceptable limits. Also, the GI absorption being very critical in drug discovery process because of its role in the bioavailability of drugs connote the process by which drugs are absorbed into the blood stream through the GI³³; therefore, hexanoic acid 1,1-dimethylethyl ester and octadecanoic acid docosyl ester that revealed high absorption in the GIT suggested they could enhance high bioavailability. Conversely, PPB as an index of drug distribution refers to the rate at which substances bind to the proteins within the blood plasma.³⁴ So, drug-plasma interaction influences several factors such as metabolism, excretion, and bioavailability; therefore, hexanoic acid 1,1-dimethylethyl ester and octa-2,4,6-triene revealed maximum PPB indices from the analysis. More so, BBB permeant as the ability of a substance to cross the BBB and enter into the central nervous system has provided the basis for drug distribution and evaluation. Therefore, artemisinin-based compounds and quinine based derivative are known to cross the BBB, necessitating their usage in the treatment of cerebral malaria³⁵; so, hexanoic acid 1,1-dimethylethyl ester, octadecanoic acid docosyl ester, and trans-β-ocimene are prospective agents for cerebral malaria treatment because they revealed their abilities in crossing the BBB. Moreover, drug metabolism is mostly mediated by a class of enzymes that belong to the cytochrome P450 family; they enhance biotransformation, detoxification, and excretion of drug metabolites in the system. However, compounds that inhibit this group of enzymes could possibly enhance toxicity in the system; however, all the compounds with the exception of octadecanoic acid docosyl ester and phytol acetate revealed that they were not inhibitors of monooxygenases and that their metabolites could not induce toxicity. Furthermore, renal clearance as drug excretion index is a pharmacokinetic parameter that is critical in the evaluation of the ease of drug metabolites elimination from the system; it also give a clue on the steady state concentration of a substance in the plasma; therefore, all the compounds with the exception of octadecanoic acid docosyl ester and glyceraldehyde revealed excellent clearance level and the possibility of their metabolites easily excreted through the kidney to avert toxicity. In addition, carcinogenicity as a toxicological parameter is used to reveal compounds that could induce carcinogenesis and mutagenesis. Here, phytol acetate, trans-β-ocimene, and glyceraldehyde revealed their non-carcinogenic potentials. Nevertheless, Lipinski's rule as a drug-likeness criteria is a set of rule used for the assessment of the resemblance of a potential drug to known drugs based on their structural and physiochemical properties³⁶; so, the rule has helped in the profiling of compounds with undesirable properties. All the compounds with the exception of octadecanoic acid docosyl ester violated at most one Lipinski's rule of five; therefore, they are drug-like molecules. Conclusively, ADMET studies is a paramount tool in drug discovery as it has enhanced the understanding of the pharmacokinetics, physicochemical, and drug-like properties of the studied compounds.

Conclusion

The EtoAc and DCM fractions of J. insularis leaf that had the best antiplasmodial therapeutic indices in P. berghei-infected mice were subsequently isolated and characterized with GC-MS to give 20 bioactive compounds with already reported antiplasmodial activities. However, the docking of these compounds with PfSHMT and PfEMP-1 generated their binding energies, bond distances, hydrogen bonds, and amino acid residues while the ADMET profiling revealed the pharmacokinetics, physicochemical, and drug-like properties of the compounds. Moreover, octadecanoic acid docosyl ester, hexanoic acid 1,1-dimethylethyl ester, and trans-β-ocimene were the lead compounds in terms of their binding energies, permeation of BBB, non-inhibition of metabolizing enzymes, ease of absorption, distribution, excretion, and non-violation of Lipinski's rules as well as favorable comparison with chloroquine in terms of their binding energies and ligand-protein interactions. Therefore, octadecanoic acid docosyl ester and hexanoic acid 1,1-dimethylethyl ester are proposed to mitigate malaria pathogenesis by competitively binding to the THF-binding sites of PfSHMT, causing the inhibition of DNA synthesis, and parasite apoptosis whereas trans-β-ocimene mediated its antiplasmodial activity through the inhibition of PfEMP-1, a receptor that mediates the attachment of pRBCs to micro-vascular endothelium, thereby, resulting in parasitaemia clearance.

Materials and Methods

Collection and Identification of Plant Material

The leaves of J. insularis were collected in January 2022 from the medicinal plants farm, University of Uyo, Uyo, Akwa Ibom State, Nigeria. The plant was identified and authenticated by Dr Uduak Eshiet, a taxonomist in the Department of Botany and Ecological Studies, University of Uyo, Nigeria. A voucher specimen (FPH 83b) of the plant was deposited in the Department of Pharmacognosy and Natural Medicine herbarium, University of Uyo, Nigeria.

Extraction

The leaves were washed with distilled water and shade dried at temperature (26 ^OC) for 2 weeks. The dried leaves (1728 g) were cut into smaller pieces, pulverized to powder, and divided into two parts. One part (497 g) was macerated in 4 L of ethanol (80%) for 72 h, while the remaining part (1231 g) was successively macerated for 72 h in 7.3 L of n-hexane (n-hex), 6.8 L of DCM, 4.1 L of ethyl acetate (EtoAc), and 3.2 L of butanol (BuOH), respectively. The filtrate of each extract and fraction was concentrated and evaporated to dryness in vacuo (40^O C) by using a rotary evaporator. The various yields were calculated as follows: 99.5 g of extract (20.01%), 196.9 g of n-hex (16.0%), 45.5 g of DCM (23.12%), 49.1 g of EtoAc (24.91%), and 70.7 g of n-BuOH (35.9%); then, the extract and fractions were stored in a refrigerator (−4 ^OC), until used for the study.³⁷

Microorganisms (Parasites)

Chloroquine-sensitive strain of Plasmodium berghei (abbreviated P. berghei) ANKA strain was obtained from the National Institute of Medical Research, Yaba, Lagos, Nigeria, and maintained by sub-passage of blood from infected to healthy mouse once every 7-8 days.

Parasite Inoculation

Each mouse used in the experiment was inoculated intraperitoneally with 0.2 mL of infected blood containing about 1 × 10⁷ P. berghei parasitized erythrocytes collected from an infected mice with 20-30% parasitaemia. The inoculum consisted of 5 × 10⁷ P. berghei-infected erythrocytes per milliliter prepared by determining both the percentage parasitemia and the erythrocytes count of the donor mouse and diluting the blood with isotonic saline in proportions indicated by both determinations.²⁰ Parasitemia was monitored by standard methods: thin blood smears were made on glass slides, fixed using methanol, stained using Giemsa stain, and parasitemia was counted using a microscope and calculated as a percentage of infected RBCs relative to the total number of cells in a microscopic field at ×100 magnification according to the formula³⁸ as given below:

\begin{aligned} P a r a s i t e m i a (%) = T o t a l n u m b e r o f p a r a s i t i s e d R B C s \times 100 \\ T o t a l n u m b e r o f R B C s \end{aligned}

Experimental Animals

Swiss Albino Wistar mice (18-25 g) of both sexes used in the study were obtained from the University of Uyo's animal house. They were kept in standard plastic cages in a well-ventilated room and acclimatized for 10 days before the experiments. The mice were fed on standard pelleted diet and water ad libitum. The care and use of animals were conducted in accordance with the National Institute of Health Guide for the Care and Use of laboratory Animals (NIH Publication, 1996). Approval (approval number: UUY/0420) for the study was obtained from the University of Uyo's Animal Ethics Committee.

Drug Administration

The extract, fractions, chloroquine, and pyrimethamine used in the study were obtained from a commercial pharmacy in Uyo, Nigeria, and administered orally with the aid of a stainless metallic feeding cannula.

Determination of Median Lethal Dose (LD₅₀)

The determination of median lethal dose (LD₅₀) of the extract was carried out in mice using oral (p.o) route by modified method of Lorke.³⁹ The mice in groups of three each were administered different doses of the extract (100-5000 mg/kg). They were observed for the manifestation of physical signs of toxicity such as writhing, decreased motor activity, decreased body/ limb tone, decreased mobility, and death. The mortality in each group within 24 h was recorded. The LD₅₀ value was calculated as geometrical means of the maximum dose producing 0% (a) and the minimum dose producing 100% mortality (b); therefore, LD₅₀ =√ab and was estimated to be = 5000 mg/kg body weight of mice.

Antiplasmodial Assay

The Evaluation of Suppressive Antiplasmodial Activities of the Drug, Extract, and Fractions of J. insularis Leaves (4-day test)

This test was used to evaluate the chemosuppressive activity of the extract, fractions, and chloroquine against early P. berghei infection in mice. This was done as described by Asanga et al⁴⁰ and Okokon et al.⁴¹ Forty-five mice were randomly divided into nine groups of five (5) mice each. On the first day (D₀), the 45 mice were inoculated with the parasite and randomly divided into the various groups and were given extract, fractions, chloroquine, and distilled water. The mice in groups (1-3) were given extract (150, 300, and 450 mg/kg) body weight of mice respectively; groups (4-7) were given n-hex, DCM, EtoAc, and n-BuOH fractions, respectively, at 300 mg/kg body weight of mice; group 8 mice (positive control) were given chloroquine at 5 mg/kg body weight of mice; then, group 9 mice (negative control) were given distilled water at 10 ml/kg body weight of mice. All the groups of mice were treated for 4 consecutive days (D₀-D₃) between 8am and 9am; on the fifth day (D₄), thin films were made from their tail blood. The films were stained with Giemsa stain to reveal parasitized erythrocytes out of 500 in a random field of the microscope. The rectal temperature of each mouse was taken daily for 5 days to monitor changes in the body temperature of the mice. The average chemosuppression of parasitemia was calculated according to the formula⁴² as follows: (average % parasitemia positive control – average % parasitemia negative control)/ (average % parasitemia negative control). The MST of the mice in each treatment group was determined over a period of 29 days (D₀-D₂₈), as follows:

(N o . o f d a y s s u r v i v e d) / (t o t a l N o . o f d a y s (29) x 100

The Evaluation of Prophylactic Antiplasmodial Activities of the Drug, Extract, and Fractions of J. insularis Leaves

This was evaluated using the method.^42,43 The mice were randomly divided into nine groups of five mice per group. Groups (1-3) were given extract (150, 300, and 450 mg/kg) body weight of mice, respectively; groups (4-7) were given n-hex, DCM, EtoAc, and n-BuOH, respectively, at 300 mg/kg body weight of mice. Mice in group 8 (positive control) were given pyrimethamine at 1.2 mg/kg body weight of mice, whereas the mice in group 9 (negative control) were given distilled water (10 ml/kg) body weight of mice. The administration of the drugs, extract, and fractions continued for 3 consecutive days (D₀-D₂); on the fourth day (D₃), the mice were inoculated with P. berghei. The parasitemia level was assessed through blood smears 72 h later and the MST of the mice was calculated over a period of 29 days.

The Evaluation of the Curative Antiplasmodial Activities of the Drug, Extract, and Fractions of J. insularis Leaves

This test was used to evaluate the schizonticidal activity of the extract, fractions, and chloroquine in established P. berghei-induced infection. This was conducted according to the methods described by Asanga et al⁴² and Okokon et al.⁴³ Forty-five (45) mice were intraperitoneally inoculated with P. berghei on the first day (D₀); 72 hours later (D₃), the mice were divided into nine groups of five mice per group. Groups (1-3) were given different doses of the extract (150, 300, and 450 mg/kg) body weight of mice, respectively. Mice in groups (4-7) were given n-hex, DCM, EtoAc, and n-BuOH fractions, respectively, at the dosage of 300 mg/kg body weight of mice. Mice in group 8 (positive control) were treated with chloroquine at 5 mg/kg body weight of mice, whereas those in group 9 (negative control) were given distilled water (10 ml/kg body weight of mice). The extract, fractions, and chloroquine were administered once daily for 5 days. Giemsa stained thin smears were prepared from tail blood samples collected on each day of treatment to monitor the parasitemia level. The rectal temperature of the mice was taken on days 0, 3, 5, and 7, whereas the MST of the mice in each group was determined over a period of 29 days (D₀-D₂₈).

Gas Chromatography-mass Spectrometry Analysis of the Fractions

GC-MS data of the fractions (DCM and ethyl acetate) (10 mg each) being the best based on their antiplasmodial therapeutic indices were recorded on an Agilent 7890 A gas chromatograph connected with an Agilent MS model 5975C MSD detector (Agilent Technologies, USA). A HP5-MS column 5% phenyl-methylpolysiloxane, 30 m × 0.25 mm × 0.25 µm was used with a helium gas flow under a pressure of 10 psi. The injector temperature was set at 280 °C. The oven temperature started from 150 °C for 3 min (min), increased to 300 °C at 10 °C/min, and held for 5 min at 300 °C. The mass spectrometer was operated using the electron ionization mode at 70 eV. The fractions were either directly injected in n-hexane or after derivatizing to form TMSi derivatives with N,O-Bis(trimethylsilyl)trifluoroacetamide as described previously.²⁰ The National Institute of Standards and Technology (NIST) database and ChemStation data system were used to compare the retention indices and mass spectrum fragmentation patterns of the isolates to identify the compounds inside them. The compounds’ molecular masses, formula, names, and retention times were all recorded.

The Assessment of the Physicochemical and Pharmacokinetics Properties of the Bioactive Compounds from J. insularis Leaves

Prediction of Target and ADMET Properties of the Ligands

This analysis was carried out using two online tools: swissADME (http://www.swissadme.ch/) and ADMET lab 2.0 (https://admetmesh.scbdd.com/)⁸; the ADMET properties of the ligands employed in this study were predicted. The different ADMET properties of the ligands and the chloroquine were predicted by using their different canonical strings or Simplified Molecular-Input Line-Entry System strings that were retrieved from the PubChem web platform (https://pubchem.ncbi.nlm.nih.gov/pccompound) in their 3D conformation. All the relevant parameters including Lipinski's rule of five and Ghose parameters were recorded. Using the SWISSADME Target Prediction tool, the targets of the different ligands were determined.⁴⁴

Target Proteins Properties and Molecular Docking of the Bioactive Compounds

In this study, the three-dimensional structures of the target proteins: recombinant serine hydroxylmethyltransferase and MC179 portion of the cysteine-rich inter-domain region of PfEmp-1 with PDB ID, 1BJ4 and 3C64, respectively, were obtained from protein data bank (PDB). The ligands and chloroquine were gotten from Pubchem (https://pubchem.ncbi.nlm.nih.gov), whereas the target proteins were retrieved and downloaded in PDB format from the Research Collaboratory for Structural Bioinformatics (RCSB) (www.RCSBPDB.org) and docked blindly.⁸ The downloaded proteins were prepared using Biovia Discovery Studio version 21.1.020298⁸; the protein preparation steps involved the removal of water molecules and native ligand groups present, as well as the addition of polar hydrogen atoms. After the proteins preparation, molecular docking analysis was carried out using PyRx.⁴⁵ The results of this analysis were evaluated based on the compound with highest negative binding energy. Also, the 2D visualization of the docking experiment was carried out by using the Biovia discovery studio as it provided an understanding of the specific amino acid residues and their types of interaction between the ligands and the target proteins. Moreover, the PyMol software⁴⁶ was employed in providing the 3D visualization of the ligand-receptor interactions, and these visualizations provided better understanding of the docking processes.

Statistical Analysis

Data collected were analyzed using one-way analysis of variance followed by Turkey's multiple comparison post-test (Graphpad prism software Inc. La Jolla, CA, USA). Values were expressed as mean ± standard error of mean (SEM) and significance relative to control were considered at P˂0.001 and P˂0.05.

Footnotes

Acknowledgements

The authors are grateful to Mr Nsikan Malachy and other staff Animal House of Pharmacology and Toxicology Department, University of Uyo for providing technical assistance as well as the editor and reviewers for the review processes.

Authors’ Contributions

Conceptualization and methodology: Enyiekere VJ, Okokon JE, and Asanga EE. Software and statistical analysis: Ekeleme CM and Asanga EE. Resources: Asanga EE, Enyiekere VJ, and Okokon JE. Writing, review, and editing: Asanga EE, Enyiekere VJ, Okokon JE, Anagboso MO, and Ise UP. Supervision: Okokon JE.

All the authors read the manuscript and the authors’ list and consented to the publication.

Availability of Data and Materials

Datasets generated in this study are available from the corresponding author on request.

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The authors received no financial support for the research, authorship, and/or publication of this article.

Ethical Approval

The NIH guidelines for the care, handling, and use of experimental animals (NIH Publication, 1996) and compliance with ARRIVE guidelines were judiciously followed. Moreover, the Animal Ethics Committee of the University of Uyo, Akwa Ibom State, Nigeria (approval number: UUY/0420) approved the experimental protocol.

Informed Consent

All the authors contributed their quota to the research, they have thoroughly read, and have approved the manuscript to be published.

ORCID iDs

Veronica James Enyiekere

Chinedum Martins Ekeleme

Edet Effiong Asanga

References

Shegokar

Pathak

. Malarial drug delivery systems. Adv Treatment of Infect Dis. 2023;20:75.

Bihonegn

Giday

Yimer

Animut

Sisay

. Antimalarial activity of hydromethanolic extract and its solvent fractions of Vernonia amygdalina leaves in mice infected with Plasmodium berghei. SAGE Open Med. 2019;2:7.

World Health Organization. World malaria report Switzerland; 2019

World Health Organization. Severe falciparum malaria. Transact Royal Soc Trop Med Hyg. 2020;94:1-90.

Egwu

Pério

Augereau

Tsamesidis

Benoit-Vical

Reybier

. Resistance to artemisinin in falciparum malaria parasites: a redox-mediated phenomenon. Free Radical Biol Med. 2022;179:317-327.

Muñoz-Durango

Maciuk

Harfouche

, et al. Detection, characterization, and screening of heme-binding molecules by mass spectrometry for malaria drug discovery. Anal Chem. 2012;84(7):3324-3329.

Kweku

Kwofie

Tetten-Tsifoanya

, et al. Targeting the Plasmodium falciparum’s thymidylate monophosphate kinase for the identification of novel antimalarial natural compounds. Front Cell Infect Microbiol. 2022;24:12.

Asanga

Ekpo

Edeke

, et al. Betulinic and ursolic acids from Nauclea latifolia roots mediate their antimalarial activities through docking with PfEMP-1 and PfPKG proteins. BMC Complement Med Ther. 2024a;24:79.

Mac-Pherson

Warrell

White

Looareesuwan

Warrell

. PfEMP-1 as malaria target protein. Am J Pathol. 2015;119:385-401.

10.

Maenpuen

Sopitthummakhun

Yuthavong

Chaiyen

Leartsakulpanich

. Characterization of Plasmodium falciparum serine hydroxymethyltransferase: a potential antimalarial target. Mol Biochem Parasitol. 2009;168(1):63-73.

11.

Chitnumsub

Ittarat

Jaruwat

, et al. The structure of Plasmodium falciparum serine hydroxymethyltransferase reveals a novel redox switch that regulates its activities. Acta Crystallogr D Biol Crystallogr. 2014;70(6):1517-1527.

12.

Maenpuen

Mee-udorn

Pinthong

, et al. Mangiferin is a new potential antimalarial and anticancer drug for targeting serine hydroxymethyltransferase. Arch Biochem Biophys. 2023;745:83.

13.

Schwertz

Witschel

Rottmann

, et al. Potent inhibitors of plasmodial serine hydroxymethyltransferase (SHMT) featuring a spirocyclic scaffold. Chem Med Chem. 2018;13(9):931-943.

14.

Asanga

Ebong

Igile

Eseyin

Essien

Thomas

Isolation of pentacyclic triterpenoid from the antiplasmodial bioactive fraction of Nauclea latifolia (Sm) roots. Trop J Pharm Res. 2022;21(3):611-618.

15.

Telefo

Moundipa

Tchouanguep

FM.

Inductive effect of the leaf mixture extract of Aloe buettneri, Justicia insularis, Dicliptera verticillata and Hibiscus macranthus on in vitro production of estradiol.. J Ethnopharmacol. 2004;91:225-230.

16.

Telefo

Tagne

Koona

Yemele

Tchouanguep

. Effect of the aqueous extract of Justicia insularis T. Anders (Acanthaceae) on ovarian folliculogenesis and fertility of female rats. Afr J Tradit Complement Altern Med. 2012;9:197-203.

17.

Adeyemi

Babatunde

Chemical composition and anti-oxidant capacity of the leaf extract of Justicia insularis

. Intern J Phys Sci. 2014;9:454-458.

18.

Wood

Yasmin-Karim

Moreau

, et al. Characterization of isolated extracts from Justicia plant leaves used as remedy for anemia. Molecules. 2020;25:534.

19.

Fadayomi

Johnson-Ajinwo

Pires

, et al. Clerodane diterpenoids from an edible plant Justicia insularis: discovery, cytotoxicity, and apoptosis induction in human ovarian cancer cells. Molecules. 2021;26:5933.

20.

Asanga

Okoroiwu

Umoh

, et al. Antiplasmodial profiling of Mangifera indica’s herbal formulation and its ability to ameliorate hematological derangements in Plasmodium berghei-infected mice. Nat Product Comm. 2023a;18(12):1-10.

21.

Asanga

Joseph

Okoroiwu

, et al. The antimalarial profiling of Mangifera indica’s herbal formulation attenuated biochemical alterations and improved hepatocytes’ histoarchitecture in Plasmodium berghei-infected mice. J Parasitic Dis. 2024b;48(2):1-153.

22.

Melariri

Campbell

Etusim

Smith

. Antiplasmodial properties and bioassay-guided fractionation of ethyl acetate extracts from Carica papaya leaves. J Parasitol Res. 2011;45:12.

23.

Kumaratilake

Robinson

Ferrante

Poulos

Antimalarial properties of n-3 and n-6 polyunsaturated fatty acids: in vitro effects on Plasmodium falciparum and in vivo effects on P. berghei. J Clin Investig. 1992;89:961-967.

24.

da Silva

EMM

Alves

Mendonca

LVH

, et al. Chemical constituents and preliminary antimalarial activity of Humiria balsamifera. Pharma Biol. 2004;42:94-99.

25.

Grace

Lategan

Graziose

Smith

Raskin

Lala

. Antiplasmodial activity of ethnobotanical plant Cassia fistula. Nat Prod Comm. 2012;7:1263-1266.

26.

Hatzakis

Opesenica

Solaja

Stratakis

Synthesis of novel polar derivatives of the antimalarial endoperoxides. ARKIVOC Arch Organic Chem. 2007;8:124-125.

27.

Belay

Gurmu

Wubneh

ZB.

Antimalarial activity of stem bark of Periploca linearifolia during early and established Plasmodium infection in mice. Evidence-Based Compl Altern Med. 2018;41(6):93-97.

28.

Jennings

Elia

. Effect of E. coli endotoxin on temperature, oxygen consumption and brown adipose tissue thermogenesis in rats and mice. Biosci Rep. 1987;7(6):517-523.

29.

Siddiqui

Trager

. Free amino-acids of blood plasma and erythrocytes of normal ducks and ducks infected with malarial parasite, Plasmodium lophurae. Nature. 1967;214(5092):1046-1047.

30.

Briand

Peter

Acute malaria: effects of P. berghei on the metabolic rate of mice. The Ohio J Sci. 1977;43:77-189.

31.

Asanga

Joseph

Umoh

, et al. New perspectives on the therapeutic potentials of bioactive compounds from Curcuma longa: targeting COX-1 & 2, PDE-4B, and antioxidant enzymes to counteract oxidative stress and inflammation. Nat Prod Commun. 2024;19(4):1-21.

32.

Juan

, et al. Hexa-fluoroisopropanol based silk fibroin coatings on AZ31 biometals with enhanced adhesion, corrosion resistance and biocompatibility. Prog Org Coat. 2023;184:107881.

33.

Azman

Sabri

Anjani

Mustaffa

Hamid

. Intestinal absorption study: challenges and absorption enhancement strategies in improving oral drug delivery. Pharmaceut. 2022;159(8):975.

34.

Smith

Kerns

. The effect of plasma protein binding on in vivo efficacy: misconception in drug discovery. Nature Reviews Drug Discov. 2010;9(12):929-939.

35.

Osigbemhe

Louis

Khan

, et al. Antibacterial potential of 2-(-(2-hydroxyphenyl)-methylidene)-amino) nicotinic acid: experimental, DFT studies, and molecular docking approach. Appl Biochem Biotech. 2022;194(12):5680-5701.

36.

Lipinski

. Lead and drug-like compounds: the rule-of-five revolution. Drug Discovery Today Technologies. 2004;1(4):337-341.

37.

Uche

Guo

Okokon

, et al. In vivo efficacy and metabolism of the antimalarial cycleanine and improved in vitro antiplasmodial activity of semisynthetic analoques. Antimicrobial Agents Chemother. 2021;65(20):e01995-20.

38.

Peters

Robinson

. The chemotherapy of rodent malaria studies on pyronaridine and other Mannich base antimalarials. Ann Trop Med Parasitol. 1992;86:455-465.

39.

Lorke

. A new approach to practical acute toxicity testing. Archives Toxicol. 1983;54:275-286.

40.

Asanga

Igile

Iwara

Ebong

Eseyin

OA.

Hematological indices of Plasmodium berghei-infected mice treated with ethanol extract and fractions of Nauclea latifolia roots. Int J Curr Microbiol Appl Sci. 2017;6(12):2546-2556.

41.

Okokon

Mobley

Edem

, et al. In vitro and in vivo antimalarial activity and chemical profiling of sugarcane leaves. Sci Rep. 2022;12(1):10250.

42.

Asanga

Okoroiwu

Edet

, et al. Antimalarial activities of Mangifera indica aqueous extract in Plasmodium berghei’s apicoplast. Trop J Pharm Res. 2023b;22(5):1007-1015.

43.

Okokon

Augustine

Mohanakrishnan

. Antimalarial, antiplasmodial, and analgesic activities of root extract of Alchornea laxiflora. Pharmaceutical Biol. 2017;55(1):1022-1031.

44.

Daina

Michielin

Zoete

. Swiss Target prediction: updated data and new features for efficient prediction of protein targets of small molecules. Nucleic Acids Res. 2019;47(1):57-64.

45.

Trott

Olson

. Autodock vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading. J Comput Chem. 2009;31(2):455-461.

46.

Lill

, Danielson ML computer-aided drug design platform using PyMOL. J Comput Aided Mol Des. 2011;25(1):13-19.

Fatty Acid Esters and Acyclic Monoterpenoid from Justicia insularis Leaf Fractions Attenuated Malaria Pathogenesis Through Docking with Plasmodium falciparum Serine Hydroxymethyl Transferase and Plasmodium falciparum Erythrocyte Membrane Protein 1 Proteins

Abstract

Background

Methods

Results

Conclusion

Keywords

Introduction

Results and Discussion

Results

The Antiplasmodial Activities of the Extract and Fractions of J. insularis Leaves

The Effect of the Extract/Fractions of J. insularis Leaves on the Rectal Temperatures of Infected Mice

GC-MS Analysis

Molecular Docking Analysis

ADMET Studies on Selected Ligands and Chloroquine

Discussion

Conclusion

Materials and Methods

Collection and Identification of Plant Material

Extraction

Microorganisms (Parasites)

Parasite Inoculation

Experimental Animals

Drug Administration

Determination of Median Lethal Dose (LD50)

Antiplasmodial Assay

The Evaluation of Suppressive Antiplasmodial Activities of the Drug, Extract, and Fractions of J. insularis Leaves (4-day test)

The Evaluation of Prophylactic Antiplasmodial Activities of the Drug, Extract, and Fractions of J. insularis Leaves

The Evaluation of the Curative Antiplasmodial Activities of the Drug, Extract, and Fractions of J. insularis Leaves

Gas Chromatography-mass Spectrometry Analysis of the Fractions

The Assessment of the Physicochemical and Pharmacokinetics Properties of the Bioactive Compounds from J. insularis Leaves

Prediction of Target and ADMET Properties of the Ligands

Target Proteins Properties and Molecular Docking of the Bioactive Compounds

Statistical Analysis

Footnotes

Acknowledgements

Authors’ Contributions

Availability of Data and Materials

Declaration of Conflicting Interests

Funding

Ethical Approval

Informed Consent

ORCID iDs

References

Determination of Median Lethal Dose (LD₅₀)