Abstract
After fixation in the presence of filipin, the membrane of the goblet cell granules of the distal colonic mucosa (guinea pig) displays, in freeze-fracture replicas, a rhombic pattern composed of intersecting "lines" of unknown nature. The filipin-cholesterol complexes of the granule membrane are ordered according to the same geometry. This arrangement--but not the "lines"--has been found for the filipin-cholesterol complexes of other membranes of the same goblet cells as well as of other colonic cells. These observations indicate that in some biological membranes cholesterol has an ordered distribution, comparable to that suggested by investigations on artificial membranes.